FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176097
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA34861.9795907937102848No Hit
CAGGAAANAGCTATGANNACACATACTCCCTTGCTCCAGTCCCTCTCAAC25991.4758911281850344No Hit
CAGGAAANAGCTATGANNACACATACTCCCTTTCTCCAGTCCCTCTCAAC25251.4338688336541794No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC23521.33562752346718No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA21451.2180786725497879No Hit
CAGGAAANAGCTATGANNACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA20171.1453914603883086No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG19201.0903081824221879No Hit
CAGGAAANAGCTATGANNGCAAAACACCTCAACACAACTGAAATCCAAGG17520.9949062164602464No Hit
CAGGAAANAGCTATGACCACACATACTCCCTTTCTCCAGTCCCTCTCAAC16160.9176760535386748No Hit
CAGGAAANAGCTATGACCACACATACTCCCTTGCTCCAGTCCCTCTCAAC16060.9119973650885592No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC15440.8767894966978427No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC14450.8205704810416986No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA13710.7785481865108435No Hit
CAGGAAANAGCTATGANNGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC13470.764919334230566No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG13450.763783596540543No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG13140.7461796623451847No Hit
CAGGAAANAGCTATGACCACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA12460.7075645808843989No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA12390.7035894989693181No Hit
CAGGAAANAGCTATGACCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC11430.6490740898482087No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCAGTCCCTCTCAAC11000.6246557295127118No Hit
CAGGAAANAGCTATGACCGCAAAACACCTCAACACAACTGAAATCCAAGG10840.6155698279925268No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG10200.5792262219117873No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTTCTCCAGTCCCTCTCAAC9790.5559435992663134No Hit
CAGGAAANAGCTATGACCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC9790.5559435992663134No Hit
CAGGAAANAGCTATGANCACACATACTCCCTTTCTCCAGTCCCTCTCAAC9760.5542399927312788No Hit
CAGGAAANAGCTATGANCACACATACTCCCTTGCTCCAGTCCCTCTCAAC9740.5531042550412557No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC9630.5468576977461286No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT9130.5184642554955508No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTGGCCGTC8900.505403272060285No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG8880.5042675343702618No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA8140.4622452398394067No Hit
CAGGAAANAGCTATGANCACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA7720.43839474834892134No Hit
CAGGAAANAGCTATGANCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG7660.43498753527885203No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA7610.4321481910537942No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACAACTGAAATCCAAGG7430.4219265518435862No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC7410.4207908141535631No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCACTGGCCGTCGTT7330.41624786339347064No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG7240.41113704378836663No Hit
CAGGAAACAGCTATGACCGCAAAACACCTCAACACAACTGAAATCCAAGG6750.3833114703828004No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCAGTCGGTACT6730.38217573269277727No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG6730.38217573269277727No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA6670.37876851962270797No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGGAACACACAACTGGC6500.3691147492575115No Hit
CAGGAAANAGCTATGANCGCAAAACACCTCAACACAACTGAAATCCAAGG6480.36797901156748836No Hit
CAGGAAANAGCTATGANCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC6440.3657075361874422No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6270.3560537658222457No Hit
CAGGAAANAGCTATGANNAGGAAGAAGCCAAGGCCCAGACTGGCCGTCGT6260.35548589697723415No Hit
CAGGAAACAGCTATGACCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC5920.3361783562468412No Hit
CAGGAAANAGCTATGANCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC5840.3316354054867488No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGGAACACACAACTGGC5720.32482097934661014No Hit
CAGGAAANAGCTATGACCAGGAAGAAGCCAAGGCCCAGACTGGCCGTCGT4880.2771199963656394No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCACTGGCCGTCGTTTTACACT4820.2737127832955701No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT4810.2731449144505585No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG4790.2720091767605354No Hit
CAGGAAANAGCTATGANNTGGAGGGAGAAGGGGAAAACTGGCCGTCGTTT4740.2691698325354776No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4700.26689835715543136No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA4620.26235540639533894No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCACTGGCCGTCGTT4600.2612196687053158No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA4470.2538373737201656No Hit
CAGGAAANAGCTATGANNCCTGACCCCATCTCACCAGAGCACCGACTGGC4420.2509980294951078No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4290.24361573450995758No Hit
CAGGAAANAGCTATGACCCTTGGTGGAGCCACTGGCCGTCGTTTTACACT4280.24304786566494602No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCAGTCGGTACT4240.2407763902848998No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4070.23112261991970334No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACAACTGAAATCCAAGG4010.227715406849634No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGCAGACGGAAATGGACTG3930.22317245608954156No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGGAACACACAACTGGC3880.22033311186448376No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT3830.21749376763942602No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG3810.2163580299494029No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3740.21238294803432198No Hit
CAGGAAANAGCTATGANCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG3700.21011147265427577No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3630.20613639073919485No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT3510.19932196459905618No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGGAACACACAACTGGC3230.18342163693873265No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3230.18342163693873265No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3190.1811501615586864No Hit
CAGGAAACAGCTATGACCAGGAAGAAGCCAAGGCCCAGACTGGCCGTCGT3120.17717507964360552No Hit
CAGGAAANAGCTATGACCTGGAGGGAGAAGGGGAAAACTGGCCGTCGTTT3110.17660721079859396No Hit
CAGGAAANAGCTATGANNTCCTGCCCCACCCTGATACAGTGACTGGCCGT3090.17547147310857084No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT3050.17319999772852462No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCACTGGCCGTCGTT3020.17149639119348994No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCAGTCGGTACT3010.17092852234847838No Hit
CAGGAAACAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA3000.17036065350346682No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACAACTGAAATCCAAGG3000.17036065350346682No Hit
CAGGAAANAGCTATGANCTTTTTGTGCCCTTCTGCCCACTGGCCGTCGTT2980.16922491581344373No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2910.16524983389836284No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACAACTGAAATCCAAGG2880.16354622736332816No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCACTGGCCGTCGTTTTACACT2850.1618426208282935No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2810.15957114544824727No Hit
CAGGAAANAGCTATGACCTGCTGGGAGAGGGGGCAGACGGAAATGGACTG2770.15729967006820106No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2740.15559606353316638No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2720.15446032584314326No Hit
CAGGAAANAGCTATGANCAGGAAGAAGCCAAGGCCCAGACTGGCCGTCGT2680.15218885046309705No Hit
CAGGAAANAGCTATGACCCCTGACCCCATCTCACCAGAGCACCGACTGGC2680.15218885046309705No Hit
CAGGAAANAGCTATGANCACTCCTCCTGGCCTTCCTCTCCAGTCGGTACT2630.14934950623803925No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2570.14594229316796992No Hit
CAGGAAACAGCTATGANCACACATACTCCCTTGCTCCAGTCCCTCTCAAC2490.1413993424078775No Hit
CAGGAAANAGCTATGANCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2420.1374242604927966No Hit
CAGGAAANAGCTATGANCCTTGGTGGAGCCACTGGCCGTCGTTTTACACT2370.1345849162677388No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTGCTGGTACTGGC2330.13231344088769256No Hit
CAGGAAACAGCTATGANCACACATACTCCCTTTCTCCAGTCCCTCTCAAC2290.13004196550764635No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT2260.12833835897261167No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2240.12720262128258858No Hit
CAGGAAANAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT2160.12265967052249613No Hit
CAGGAAANAGCTATGACCTCCTGCCCCACCCTGATACAGTGACTGGCCGT2110.11982032629743834No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT2090.11868458860741524No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2080.11811671976240368No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2070.11754885091739213No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGGAAGGAACTGGC2040.11584524438235747No Hit
NAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2020.11470950669233435No Hit
CAGGAAANAGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1970.11187016246727655No Hit
CAGGAAANAGCTATGANNTCAACACAACTGAAATCCAAGGTACTGGCCGT1970.11187016246727655No Hit
CAGGAAACAGCTATGANCTTCATACAGAGATACAAAGGCAACTGGCCGTC1920.10903081824221877No Hit
CAGGAAACAGCTATGANCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG1910.10846294939720721No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1840.10448786748212632No Hit
CAGGAAANAGCTATGANNTCCCACCACTGACTCCAGGGTACTGGCCGTCG1830.10391999863711478No Hit
CAGGAAACAGCTATGACCTGGAGGGAGAAGGGGAAAACTGGCCGTCGTTT1830.10391999863711478No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1820.10335212979210322No Hit
CAGGAAANAGCTATGANCTGGAGGGAGAAGGGGAAAACTGGCCGTCGTTT1820.10335212979210322No Hit
CAGGAAANAGNTATGANNACACATACTCCCTTTCTCCAGTCCCTCTCAAC1790.10164852325706855No Hit
NAGGAAANAGCTATGANNACACATACTCCCTTGCTCCAGTCCCTCTCAAC1780.10108065441205699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)