FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161783
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA39352.432270386876248No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT37172.297521989331388No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC33912.0960175049294425No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA32141.9866116959136622No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG29651.8327018289931576No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT29081.7974694498185844No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA28881.7851072115117161No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT25931.6027641964854158No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC25741.591020070093891No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC24631.5224096474907747No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG24401.5081930734378766No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA24001.483468596824141No Hit
TGTAAAACGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG22671.4012597120834698No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT22671.4012597120834698No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG22271.376535235469734No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC20851.2887633434909724No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA20331.2566215238931162No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC19971.234369494940754No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC19921.231278935364037No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT19731.2195348089725124No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC17661.0915856424964305No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT17631.0897313067504002No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG17041.05326270374514No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC16711.0328650105388082No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG15760.9741443785811859No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT15610.9648726998510351No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT14840.9172780823695939No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT14830.9166599704542505No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT14710.9092426274701297No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC14640.9049158440627261No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT14230.879573255533647No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC13550.8375416452902962No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC12500.7726398941792401No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG11930.7374075150046667No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC11730.725045276697799No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA11660.7207184932903952No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT11620.7182460456290216No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC11610.7176279337136782No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC11320.6997026881687198No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC11290.6978483524226897No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA10380.6416001681264409No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT10190.6298560417349166No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA10130.6261473702428562No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG9970.6162575795973618No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA9480.5859700957455357No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG9040.5587731714704264No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC8820.5451747093328718No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT8710.5383754782640945No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTCTAGCCTGGGCA8500.5253951280418833No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCATGTAAAACGACG8500.5253951280418833No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC8470.523540792295853No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA8130.5025249871741777No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG8080.4994344275974608No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG8050.4975800918514306No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC7560.4672926079996044No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT7540.4660563841689176No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC7090.4382413479784649No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT6930.42835155733297076No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG6890.42587910967159714No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG6850.4234066620102236No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC6530.40362708071923503No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCTTGTGACAATGA6480.4005365211425181No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG6080.37581204452878236No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC6020.37210337303672203No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA5890.3640679181372579No Hit
TGTAAAACGACGGCCAGTCGCAGAAGCAAGGGGCTGAAAAGATCTGAAGG5590.3455245606769562No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCACTCACT5270.32574497938596764No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT5260.3251268674706242No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG5240.3238906436399374No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA5160.3189457483171903No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG5140.3177095244865035No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGCTGTGTATTACT5050.31214651724841297No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG4980.30781973384100925No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC4920.3041110623489489No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA4760.29422127170345463No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGCGTAGAACACT4210.2602251163595681No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT4160.2571345567828511No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGTT4120.2546621091214776No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG3710.22931952059239846No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA3510.21695728228553063No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG3480.21510294653950043No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGGGGGTCATAGCTGT3330.20583126780934954No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG3140.19408714141782513No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA3130.19346902950248174No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCCGCAGTGGAGGAT3120.19285091758713832No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGAAATCAGGCTG3000.18543357460301763No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGGGCTGAAAAGAT2980.18419735077233082No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTTTAGCCTGGGCA2970.18357923885698746No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTTCTGGGTC2960.18296112694164407No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACGTGGTT2930.18110679119561388No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCATGTAAAACGACG2850.17616189587286674No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT2840.17554378395752335No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCTGCAGTGGAGGAT2770.17121700055011962No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCAGACCCAAACT2770.17121700055011962No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAAAGTGACAGGTAG2680.16565399331202907No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT2620.1619453218199687No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG2560.15823665032790837No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC2560.15823665032790837No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCTGGGAGAGT2360.14587441202104054No Hit
TGTAAAACGACGGCCAGTGGGGACAAGGGTGGCTTTGTAAATAGCAGCAA2350.14525630010569715No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATGCAGTTTTAAG2300.14216574052898018No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA2210.13660273329088965No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCTCTTTCTCTAG2190.13536650946020287No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT2000.12362238306867841No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGACACGGGGGATG1950.12053182349196145No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGAAATCAGGCT1850.11435070433852754No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGACCCTTCCAGGA1830.11311448050784076No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTCCTGCTCA1750.1081695851850936No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA1710.10569713752372005No Hit
TGTAAAACGACGGCCAGTGGAGCTGGAGACACAGCTGGGTCATAGCTGTT1640.1013703541163163No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC1630.10075224220097291No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)