FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134996
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG18251.3518919079083824No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA16041.1881833535808468No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC11100.8222465850840025No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG10400.7703931968354618No Hit
CAGGAAANAGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT9580.7096506563157426No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT9150.6777978606773534No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA8840.6548342173101425No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG7500.5555720169486503No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA7230.5355714243384989No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG7200.5333491362707043No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC6630.4911256629826069No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC6440.4770511718865745No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG6390.47334735844025005No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT6280.4651989688583365No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC6260.4637174434798068No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT6210.4600136300334825No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC6210.4600136300334825No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT6180.4577913419656879No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG6100.45186524045156895No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA5830.43186464784141754No Hit
CAGGAAANAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC5740.42519778363803373No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG5530.40964176716347156No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA5450.4037156656493525No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG5350.3963080387567039No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG5250.3889004118640552No Hit
CAGGAAANAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT5180.38371507303920116No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC4860.3600106669827254No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT4840.3585291416041957No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC4800.3555660908471362No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC4570.3385285489940443No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4540.33630626092624966No Hit
CAGGAAANAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG4150.3074165160449198No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC4070.30149041453080094No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG4060.300749651841536No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT4010.2970458383952117No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT4010.2970458383952117No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG3990.29556431301668196No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3830.2837121099884441No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3680.2726006696494711No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA3660.27111914427094136No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTTGGGAAAGACTG3530.2614892293104981No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT3450.25556312779637913No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT3440.25482236510711426No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC3410.2526000770393197No Hit
CAGGAAANAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT3410.2526000770393197No Hit
CAGGAAANAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT3410.2526000770393197No Hit
CAGGAAANAGCTATGANCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC3400.2518593143500548No Hit
CAGGAAANAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC3390.25111855166078995No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC3350.24815550090373045No Hit
CAGGAAANAGCTATGANCCCCGTGACAGCTCCTGCAATTACAAAATGGCT3340.2474147382144656No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3310.24519245014667101No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT3220.23852558594328724No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT3180.23556253518622772No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA3160.23408100980769803No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC3150.23334024711843313No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3100.22963643367210881No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGCAGAGGGTTGGGAAAGACTG3070.2274141456043142No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC3010.22296956946872498No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2990.2214880440901953No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC2880.2133396545082817No Hit
CAGGAAANAGCTATGANNGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG2870.21259889181901687No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2850.21111736644048715No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2820.20889507837269253No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2760.20445050223710332No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC2720.20148745148004388No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG2700.2000059261015141No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2680.1985244007229844No Hit
CAGGAAANAGCTATGANCACAAACGCAAATGGCTTTTAGCATTTCACAGG2660.19704287534445467No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2630.19482058727666005No Hit
CAGGAAANAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2610.19333906189813033No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC2580.1911167738303357No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2550.18889448576254111No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC2540.18815372307327624No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2520.18667219769474652No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC2520.18667219769474652No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCGCATTGTAGTCAA2450.18148685886989244No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2440.1807460961806276No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2410.17852380811283297No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2410.17852380811283297No Hit
CAGGAAANAGCTATGANCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2410.17852380811283297No Hit
CAGGAAANAGCTATGANNCCACCCTCATGCCTAACCCCTATTTTCCAAAG2400.1777830454235681No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2400.1777830454235681No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2380.17630152004503838No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2380.17630152004503838No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2360.17481999466650863No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2310.1711161812201843No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC2300.17037541853091945No Hit
CAGGAAANAGCTATGANCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2300.17037541853091945No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA2280.1688938931523897No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT2260.16741236777385995No Hit
CAGGAAANAGCTATGANCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT2250.1666716050845951No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2230.16519007970606536No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2150.15926397819194643No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2140.15852321550268156No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2130.15778245281341668No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2100.1555601647456221No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2070.1533378766778275No Hit
CAGGAAANAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG2060.15259711398856263No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2000.14815253785297341No Hit
CAGGAAANAGCTATGANNCTTGCTTCTGCGGACACTGACTGGCCGTCGTT2000.14815253785297341No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2000.14815253785297341No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGTAGAGTGTTGGGAAAGACTG1970.14593024978517882No Hit
CAGGAAANAGCTATGANNACAGGGGCCTCTTAAAACTGCATGTGTTCAGC1940.1437079617173842No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1930.14296719902811936No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1920.14222643633885448No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT1900.14074491096032477No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC1860.13778186020326527No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTTTCAGAGATGGT1820.1348188094462058No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1780.13185575868914634No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA1770.13111499599988147No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1740.12889270793208688No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1730.12815194524282203No Hit
CAGGAAANAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1720.12741118255355713No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1690.12518889448576254No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1690.12518889448576254No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1690.12518889448576254No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1680.12444813179649768No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1680.12444813179649768No Hit
CAGGAAANAGCTATGANCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1670.1237073691072328No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1670.1237073691072328No Hit
CAGGAAANAGCTATGANCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1660.12296660641796794No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1570.11629974221458413No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGCAGAGGGTTGGGAAAGACTG1570.11629974221458413No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1560.11555897952531928No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1540.11407745414678952No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1530.11333669145752467No Hit
CAGGAAANAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG1530.11333669145752467No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1520.1125959287682598No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1520.1125959287682598No Hit
CAGGAAANAGCTATGANCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1520.1125959287682598No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1500.11111440338973007No Hit
CAGGAAANAGCTATGANCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1500.11111440338973007No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG1490.1103736407004652No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1490.1103736407004652No Hit
CAGGAAANAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1490.1103736407004652No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC1480.10963287801120034No Hit
CAGGAAANAGCTATGANCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1480.10963287801120034No Hit
CAGGAAANAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT1480.10963287801120034No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1470.10889211532193546No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1460.1081513526326706No Hit
CAGGAAANAGCTATGANCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1430.105929064564876No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1410.10444753918634626No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC1390.10296601380781652No Hit
CAGGAAANAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG1390.10296601380781652No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1380.10222525111855166No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1380.10222525111855166No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT1360.10074372574002194No Hit
CAGGAAANAGCTATGANCGCAGCAAGAGACCCAGACCCACCCGACTGGCC1350.10000296305075705No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)