FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64267
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA9671.5046602455381455No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC9351.4548679726764902No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7711.199682574260507No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5260.8184604851634587No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC5120.7966763657864846No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4910.7640001867210232No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4550.7079838797516611No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT4430.6893117774285403No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4430.6893117774285403No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT4330.673751692159273No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3870.6021752999206436No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG3730.5803911805436694No Hit
NAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3690.5741671464359624No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA3610.5617190782205487No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA3400.5290428991550873No Hit
NAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3320.5165948309396736No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT3150.4901426859819192No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2950.4590225154433847No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2780.4325703704856303No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT2690.41856629374328974No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC2610.4061182255278759No Hit
NAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2590.40300620847402246No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2560.3983381828932423No Hit
CAGGAAANAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2530.3936701573124621No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2510.3905581402586086No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2420.3765540635162681No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2420.3765540635162681No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA2380.37033002940856113No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT2320.3609939782470008No Hit
NAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2200.3423218759238801No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT2200.3423218759238801No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2160.33609784181617314No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2090.3252057821276861No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC2080.32364977360075936No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2080.32364977360075936No Hit
CAGGAAANAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA2060.32053775654690586No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2040.3174257394930524No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1980.30808968833149203No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT1920.2987536371699317No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC1910.297197628643005No Hit
CAGGAAACAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC1890.29408561158915153No Hit
NAGGAAANAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1820.2831935519006644No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT1820.2831935519006644No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1810.2816375433737377No Hit
NAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1800.280081534846811No Hit
CAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1790.27852552631988425No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC1770.2754135092660308No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1750.2723014922121773No Hit
NAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT1720.26763346663139714No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1710.2660774581044704No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA1700.26452144957754364No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1690.262965441050617No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1680.26140943252369025No Hit
NAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1660.2582974154698368No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1650.25674140694291003No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1640.2551853984159833No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1600.2489613643082764No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG1600.2489613643082764No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1590.24740535578134967No Hit
NAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1580.24584934725442295No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1550.24118132167364278No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1500.23340127903900915No Hit
NAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1480.23028926198515567No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1470.22873325345822895No Hit
NAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1470.22873325345822895No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1460.2271772449313022No Hit
NAGGAAANAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA1460.2271772449313022No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1440.22406522787744876No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1440.22406522787744876No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC1420.22095321082359534No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1420.22095321082359534No Hit
CAGGAAANAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1410.2193972022966686No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1390.21628518524281515No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC1380.21472917671588843No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1380.21472917671588843No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1370.21317316818896168No Hit
CAGGAAANAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1370.21317316818896168No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA1350.21006115113510823No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1340.20850514260818148No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA1330.20694913408125476No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1320.20539312555432804No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG1290.20072509997354784No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1280.19916909144662115No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA1260.19605707439276768No Hit
CAGGAAANAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA1250.19450106586584096No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1240.19294505733891423No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1230.19138904881198748No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT1230.19138904881198748No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1210.18827703175813404No Hit
CAGGAAANAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC1200.1867210232312073No Hit
CAGGAAANAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC1200.1867210232312073No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1190.18516501470428057No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCCCCGGGAGCTG1190.18516501470428057No Hit
NAGGAAANAGNTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1170.18205299765042712No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1150.17894098059657368No Hit
NAGGAAANAGNTATGANCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1140.17738497206964693No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1130.1758289635427202No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1120.17427295501579348No Hit
NAGGAAANAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1100.17116093796194004No Hit
NAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1090.16960492943501332No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1090.16960492943501332No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCACAGGTCCCACCC1060.16493690385423312No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1050.16338089532730637No Hit
CAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1050.16338089532730637No Hit
CAGGAAANAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1050.16338089532730637No Hit
CAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1030.16026887827345293No Hit
CAGGAAANAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1020.1587128697465262No Hit
NAGGAAANAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1000.15560085269267276No Hit
CAGGAAANAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA1000.15560085269267276No Hit
CAGGAAANAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT1000.15560085269267276No Hit
NAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG990.15404484416574601No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG990.15404484416574601No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC980.15248883563881932No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC970.15093282711189257No Hit
NAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG970.15093282711189257No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT970.15093282711189257No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC960.14937681858496585No Hit
CAGGAAANAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC950.14782081005803913No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA950.14782081005803913No Hit
CAGGAAANAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT940.14626480153111238No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT930.14470879300418565No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC930.14470879300418565No Hit
NAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC930.14470879300418565No Hit
CAGGAAANAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG930.14470879300418565No Hit
CAGGAAANAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC920.14315278447725893No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC880.13692875036955202No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG870.1353727418426253No Hit
CAGGAAANAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG870.1353727418426253No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT860.13381673331569857No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG850.13226072478877182No Hit
CAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT850.13226072478877182No Hit
CAGGAAANAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA850.13226072478877182No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT830.1291487077349184No Hit
CAGGAAANAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA830.1291487077349184No Hit
CAGGAAANAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG820.12759269920799166No Hit
NAGGAAANAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT810.12603669068106493No Hit
NAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC810.12603669068106493No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCTGCCAGGAGCAGG810.12603669068106493No Hit
CAGGAAANAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA810.12603669068106493No Hit
NAGGAAANAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC800.1244806821541382No Hit
NAGGAAANAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG790.12292467362721148No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC790.12292467362721148No Hit
NAGGAAANAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA790.12292467362721148No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT790.12292467362721148No Hit
CAGGAAANAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG780.12136866510028474No Hit
CAGGAAANAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG780.12136866510028474No Hit
NAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC770.11981265657335803No Hit
NAGGAAANAGNTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT760.1182566480464313No Hit
NAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG760.1182566480464313No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG760.1182566480464313No Hit
CAGGAAANAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG750.11670063951950457No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT750.11670063951950457No Hit
NAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC750.11670063951950457No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG740.11514463099257784No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT730.1135886224656511No Hit
NAGGAAANAGNTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC730.1135886224656511No Hit
NAGGAAANAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC720.11203261393872438No Hit
CAGGAAANAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG720.11203261393872438No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA710.11047660541179767No Hit
NAGGAAANAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT710.11047660541179767No Hit
CAGGAAANAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC710.11047660541179767No Hit
NAGGAAANAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC700.10892059688487093No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA690.10736458835794421No Hit
NAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG690.10736458835794421No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT670.10425257130409074No Hit
NAGGAAANAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA660.10269656277716402No Hit
CAGGAAANAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT660.10269656277716402No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGATGCTGCCTCCT650.10114055425023728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)