FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160932
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG29021.803246091516914No Hit
TGTAAAANGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC27691.720602490492879No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG26981.6764844779161385No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC24701.5348097333035071No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC17991.1178634454303682No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG16971.0544826386299804No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG16821.0451619317475704No Hit
TGTAAAANGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA16811.044540551288743No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT16261.01036462605324No Hit
TGTAAAANGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG15900.987994929535456No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC15890.9873735490766287No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC15870.986130788158974No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG15420.958168667511744No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT15210.9451196778763702No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC15200.9444982974175429No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT15190.9438769169587155No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC14740.9159147963114856No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA14240.884845773370119No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA14170.8804961101583276No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG14020.8711754032759178No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT13880.8624760768523352No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC13830.8593691745581986No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG13630.8469415653816519No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA12970.805930455099048No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA12090.751248974722243No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA11990.7450351701339697No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC11780.7319861804985957No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG11700.727015136827977No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC11620.7220440931573584No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC11610.7214227126985311No Hit
TGTAAAANGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA11480.7133447667337758No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG11430.7102378644396391No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC11270.7002957770984017No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC10610.659284666815798No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC10360.6437501553451147No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC10220.6350508289215321No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC10040.6238659806626401No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG10020.6226232197449855No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC9880.6139238933214028No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA9710.6033604255213382No Hit
TGTAAAANGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA9560.5940397186389282No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC9470.5884472945094823No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG9420.5853403922153456No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG9410.5847190117565183No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC9320.5791265876270723No Hit
TGTAAAANGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG9250.5747769244152809No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG9160.569184500285835No Hit
TGTAAAANGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT9030.5611065543210797No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG8950.5561355106504611No Hit
TGTAAAANGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT8940.5555141301916336No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG8900.5530286083563244No Hit
TGTAAAACGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA8840.5493003256033605No Hit
TGTAAAANGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC8800.5468148037680511No Hit
TGTAAAANGACGGCCAGTAGGAGGGCGGAGGAGGAAAGCAGGTTTGCTCT8590.5337658141326772No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT8070.501454030273656No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG8040.49958988889717393No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC7970.4952402256853826No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC7940.4933760843089006No Hit
TGTAAAANGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC7870.4890264210971093No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC7730.4803270946735267No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG7620.4734919096264261No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT7610.47287052916759875No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC7570.47038500733228944No Hit
TGTAAAACGACGGCCAGTAGGAGGGCGGAGGAGGAAAGCAGGTTTGCTCT7410.46044291999105214No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC6900.4287525165908582No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG6820.4237814729202396No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG6700.4163249074143116No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG6580.4088683419083836No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT6480.4026545373201104No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT6400.39768349364949174No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG6100.3790420798846718No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG6060.3765565580493625No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG5860.3641289488728158No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT5820.3616434270375065No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG5820.3616434270375065No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG5740.3566723833668879No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG5720.3554296224492332No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG5710.3548082419904059No Hit
TGTAAAANGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT5660.3517013396962692No Hit
TGTAAAANGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA5610.3485944374021326No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5610.3485944374021326No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT5490.34113787189620465No Hit
TGTAAAANGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG5450.3386523500608953No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG5430.33740958914324065No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT5420.3367882086844133No Hit
TGTAAAANGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG5330.33119578455496734No Hit
TGTAAAANGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA5290.328710262719658No Hit
TGTAAAANGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT5270.32746750180200335No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC5270.32746750180200335No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG4990.3100688489548381No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG4960.3082047075783561No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG4950.30758332711952874No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG4850.30136952253125543No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA4840.3007481420724281No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG4830.3001267616136008No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG4830.3001267616136008No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT4690.2914274351900181No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG4680.2908060547311908No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT4660.2895632938135361No Hit
TGTAAAANGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC4660.2895632938135361No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4580.2845922501429175No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA4430.2752715432605075No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG4310.2678149777545796No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC4260.26470807546044295No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT4240.26346531454278826No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT4120.2560087490368603No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3850.23923147664852235No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC3700.2299107697661124No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGGGGACCTGCTGGAG3560.22121144334252976No Hit
TGTAAAANGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG3350.2081624537071558No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGGGGACCTGCTGGAG3350.2081624537071558No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC3270.20319141003653715No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT3100.19262794223647253No Hit
TGTAAAANGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA2960.1839286158128899No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2830.1758506698481346No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG2600.161558919295106No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA2320.14416026644794075No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA2300.1429175055302861No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2300.1429175055302861No Hit
TGTAAAANGACGGCCAGTGCCAATCCTGAGGCCATCAAAGTGACAGGTAG2230.13856784231849475No Hit
TGTAAAANGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC2130.13235403773022147No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG2040.12676161360077548No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG2020.12551885268312082No Hit
TGTAAAANGACGGCCAGTGACTGTAGGTTGGGCTCCCCATGTGCTGTGCC1980.1230333308478115No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC1970.12241195038898416No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT1970.12241195038898416No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAAAGTGACAGGTAG1970.12241195038898416No Hit
TGTAAAANGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT1910.11868366763602019No Hit
TGTAAAANGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1910.11868366763602019No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA1880.1168195262595382No Hit
TGTAAAANGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1860.11557676534188353No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1840.11433400442422888No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA1770.10998434121243755No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1700.10563467800064623No Hit
TGTAAAANGACGGCCAGTTGGAGTGTCTAGGTTTGAAAGCTGCTGGTCAT1690.1050132975418189No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1670.10377053662416423No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCATGTGCTGTGCC1670.10377053662416423No Hit
TGTAAAANGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1630.1012850147888549No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)