FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260540
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC211818.129653795962232No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC125844.829968526905658No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC109474.201658094726338No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG92083.534198203730713No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG90043.455899286098104No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG64152.462193904966608No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG62102.3835111691103092No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA51511.9770476702233821No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA42831.643893452061104No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC39161.5030321639671451No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG35941.3794426959392032No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG32361.2420357718584478No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC32031.2293697704767022No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT29801.143778306593997No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC29671.1387886696860365No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC29141.118446303830506No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT27361.0501266600138175No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC25240.9687571965916941No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG25130.9645351961311123No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC24900.9557073769862593No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG24760.9503339218546097No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA24080.92423428264374No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG21300.8175328164581254No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC20030.7687879020495894No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT19050.7311737161280417No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC17920.6878022568511553No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA17430.6689951638903815No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG16370.6283104321793199No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG15110.5799493359944731No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG13970.5361940584938973No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG13480.5173869655331235No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG12980.49819605434866043No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG12960.4974284179012819No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG12340.47363168803254785No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG11970.45943041375604515No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA11110.4264220465187687No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT10810.4149074998080909No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG10710.41106931757119824No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC9870.3788285867813004No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA8680.33315421816227836No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA8640.3316189452675213No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA8220.31549857987257235No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG7580.29093421355645965No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA7370.28287403085898516No Hit
TGTAAAACGACGGCTAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC7120.27327857526675364No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC7000.2686727565824825No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG6690.25677439164811544No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC6680.25639057342442617No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT6310.24218929914792353No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG6080.23336148000307055No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA6050.23221002533200277No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG5040.19344438473938744No Hit
TGTAAAACGACGGCCAGTTCAGGGAGGCGGGGCTCGTTCCACAGGGACAC4790.18384892914715592No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG4700.18039456513395258No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT4600.17655638289705997No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA4490.17233438243647808No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC4350.16696092730482842No Hit
TGTAAAACGACGGCCAGTTCAGGGAGGCGGGGCTCGTGCCACAGGGACAC4030.15467874414677207No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC3940.15122438013356873No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT3870.14853765256774393No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACACCCTGGAGGAGATGAAC3410.13088201427803792No Hit
TGTAAAACGACGGCTAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC3380.12973055960697014No Hit
TGTAAAACGACGGCTAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG3240.12435710447532047No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3140.12051892223842788No Hit
TGNAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC3050.11706455822522455No Hit
TGNAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCTGGGACAGCTGCC3030.11629692177784602No Hit
TGTAAAACGACGGCTAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC3000.11514546710677824No Hit
TGTAAAACGACGGCTAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG2930.11245873954095341No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACACCCTGGAGGAGACGAAC2870.11015583019881785No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGGCTGGAGTGC2840.10900437552775007No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACGCCTTC2840.10900437552775007No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGACTGCGGGGGTACCG2800.10746910263299302No Hit
TGNAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCCGCGGGGGTACCG2790.10708528440930376No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG2770.10631764796192524No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC2770.10631764796192524No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC2650.10171182927765411No Hit
TGNAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC2610.10017655638289706No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC2610.10017655638289706No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)