FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260540
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC65582.517079910954172No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT60652.3278575266753667No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC57782.217701696476549No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA56742.1777846012128657No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA48651.8672756582482537No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC45161.7333230981807015No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT38231.467337069164044No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT27391.0512781146848853No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC24780.9511015583019882No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT23920.9180931910647119No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA22510.863974821524526No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC21560.8275120902740463No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG19960.7661011744837645No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA19170.7357795348123128No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT19000.7292546250095955No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG18100.694710984877562No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA17220.6609349811929071No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC16880.6478851615874722No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT16860.6471175251400937No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG14660.5626775159284563No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG13930.5346587855991403No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC12400.4759345973746834No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG12170.4671067782298304No Hit
CAGGAAANAGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG12140.4659553235587626No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG11400.4375527750057573No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC11070.42488677362401167No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG9650.3703845858601367No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC9280.356183311583634No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT8960.34390112842557763No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT8780.33699240039917094No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8680.33315421816227836No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT8310.3189529438857757No Hit
CAGGAAANNGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT8270.31741767099101864No Hit
CAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC7630.29285330467490595No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT7450.28594457664849926No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7370.28287403085898516No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7360.2824902126352959No Hit
NAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC7310.2805711215168496No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG6960.2671374836877255No Hit
NAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC6800.2609963921086973No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6760.25946111921394027No Hit
NAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC6750.259077300990251No Hit
NAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA6650.2552391187533584No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA6600.25332002763491207No Hit
CAGGAAANAGCTATGANNTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA6550.2514009365164658No Hit
CAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC6530.25063330006908724No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG6420.24641129960850539No Hit
CAGGAAANNGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT6390.2452598449374376No Hit
NAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC6290.241421662700545No Hit
CAGGAAANNGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG6260.2402702080294772No Hit
CAGGAAANAGCTATGANNGGGTCCCACCCATGCTGAAAGTCCACCTTCCA6190.23758348046365244No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG6040.23182620710831353No Hit
NAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA5960.22875566131879943No Hit
CAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA5870.2253012973055961No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA5820.2233822061871498No Hit
NAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA5610.2153220234896753No Hit
NAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA5580.21417056881860752No Hit
CAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA5420.20802947723957935No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT5330.204575113226376No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC5240.20112074921317266No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT5240.20112074921317266No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCAG5240.20112074921317266No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT5170.19843402164734783No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG5140.19728256697628005No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG5080.1949796576341445No Hit
CAGGAAANNGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG5070.19459583941045522No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG5050.1938282029630767No Hit
NAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4960.19037383894987334No Hit
NAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4930.18922238427880556No Hit
CAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4760.18269747447608814No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC4740.1819298380287096No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC4620.17732401934443848No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC4570.17540492822599218No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC4460.1711829277654103No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC4430.17003147309434252No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG4410.169263836646964No Hit
NAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4350.16696092730482842No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG4330.1661932908574499No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG4330.1661932908574499No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4260.16350656329162508No Hit
CAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4200.16120365394948952No Hit
NAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4140.15890074460735396No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4080.1565978352652184No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4040.15506256237046134No Hit
CAGGAAANNGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG3970.1523758348046365No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3880.14892147079143317No Hit
CAGGAAANNGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC3870.14853765256774393No Hit
CAGGAAACAGCNATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3870.14853765256774393No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3800.1458509250019191No Hit
CAGGAAANAGCTATGANNCGGGACCCAAAGTTCTGCGTGATGGTCAGCAC3740.14354801565978353No Hit
CAGGAAACAGCNATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3670.1408612880939587No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA3650.14009365164658016No Hit
NAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3610.13855837875182314No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG3600.13817456052813387No Hit
CAGGAAACAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3590.1377907423044446No Hit
CAGGAAACNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3530.13548783296230904No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3450.13241728717279497No Hit
CAGGAANNNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3430.13164965072541643No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3380.12973055960697014No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCAGAGAGTGATAT3330.12781146848852382No Hit
NAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3320.12742765026483457No Hit
CAGGAANNNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3260.125124740922699No Hit
CAGGAANNNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3240.12435710447532047No Hit
NAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3220.12358946802794196No Hit
CAGGAAACNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3220.12358946802794196No Hit
CAGGAAACNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3210.12320564980425269No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT3210.12320564980425269No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT3180.12205419513318491No Hit
NAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3160.12128655868580639No Hit
CAGGAANNNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3140.12051892223842788No Hit
CAGGAAACAGCTATGANCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3110.11936746756736011No Hit
NAGGAAANAGCTATGANCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3110.11936746756736011No Hit
CAGGAAANNGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC3070.11783219467260307No Hit
CAGGAAANNGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2980.11437783065939972No Hit
CAGGAAANNGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT2910.1116911030935749No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2910.1116911030935749No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC2890.11092346664619637No Hit
CAGGAAACAGCTATGANCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2880.1105396484225071No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT2880.1105396484225071No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2800.10746910263299302No Hit
CAGGAAACAGCNATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2800.10746910263299302No Hit
CAGGAAACNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2780.1067014661856145No Hit
CAGGAAACAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2770.10631764796192524No Hit
NAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2740.10516619329085745No Hit
CAGGAAANNGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2740.10516619329085745No Hit
CAGGAAACAGCNATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2660.10209564750134337No Hit
CAGGAAACAGCTATGANCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2620.10056037460658633No Hit
CAGGAAACNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2620.10056037460658633No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)