FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114586
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT38703.3773759447052867No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA35103.063201438221074No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT35033.057092489483881No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG34352.997748416036863No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA32192.8092437121463356No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC31322.7333182064126507No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT27742.4208891138533506No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA24962.1782765782905416No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG18801.6406890894175554No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGGCCGTCGTTT18091.5787268950831692No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC17181.4993105614996596No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA16851.4705112317386069No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA16271.4198942279161504No Hit
CAGGAAACAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC14901.3003333740596583No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG14291.2470982493498333No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA14101.2305168170631664No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT11681.019321732148779No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC10320.9006335852547431No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC9460.8255807864835145No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT9450.8247080795210584No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT9400.8203445447087777No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA9240.8063812333094793No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG8590.7496552807498298No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC8550.7461644529000052No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT8110.7077653465519348No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC7800.6807114307157943No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC7340.6405669104428114No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT7080.6178765294189517No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACACAGGAAACAGCT6730.5873317857329865No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT6460.5637686977466706No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG5780.5044246242996527No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA5740.500933796449828No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT5590.48784319201298587No Hit
CAGGAAACAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT5440.4747525875761437No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG4990.43548077426561704No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACACACGGC4870.4250082907161433No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC4760.4154085141291257No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC4760.4154085141291257No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA4710.41104497931684497No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCAGTAATGGA4700.41017227235438886No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT4670.40755415146702045No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT4550.3970816679175466No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT4550.3970816679175466No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA4410.3848637704431606No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAACAGGGACGCA4400.38399106348070444No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC4120.35955526853193237No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA4080.35606444068210774No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT4000.3490827849824586No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT3890.339483008395441No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCCAGCTGGA3860.33686488750807253No Hit
CAGGAAACAGCTATGACCTTCCTGATGGGATTCAGCCATCAACAGTAGGA3830.33424676662070407No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA3530.3080655577470197No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC3410.29759307419754594No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG3320.2897387115354406No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCAACAGGGAC3190.2783935210235107No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT3180.27752081406105455No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG3160.2757754001361423No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAGAAGTCTTTTAA3010.2626847956993001No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCAAACTTGG3000.2618120887368439No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2940.25657584696210706No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGTGAGGACAGAT2790.24348524252526485No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC2740.23912170771298413No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACGCACGGC2540.22166756846386118No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG2430.2120677918768436No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT2420.21119508491438746No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCACTAGACTCT2300.20072260136491368No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT2220.1937409456652645No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2220.1937409456652645No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2220.1937409456652645No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACACTCCCCGGTGAC2140.18675928996561536No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCCGCCTGGCTTG2120.18501387604070307No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT2010.17541409945368544No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG1960.1710505646414047No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTTGGGTCTGGGGC1920.16755973679158012No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACACGGGAAACCTCA1860.16232349501684326No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1820.15883266716701866No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACACACACTGGGGCC1770.15446913235473791No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGATCAGGGTGG1650.14399664880526417No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGATTATCATCC1610.1405058209554396No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGAAGAATCGC1600.13963311399298345No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCAGGAAACAGCT1560.13614228614315885No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCCTGCCCCCTGCA1560.13614228614315885No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGAACACAGGAT1390.12130626778140435No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCATGCCTGTAA1360.11868814689403592No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT1360.11868814689403592No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTACTCGGGAGGC1200.10472483549473757No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1200.10472483549473757No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGGGCACGGTGG1180.10297942156982527No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATATGAATTTCTGT1170.10210671460736914No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCAGAGGGGTC1170.10210671460736914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)