FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134763
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG19831.4714721399790744No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT18581.3787167100762079No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG17671.311190757106921No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG16801.246632977894526No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT16641.234760282866959No Hit
TGTAAAANGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC15961.1843013289997997No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG15581.1561036783093281No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA14701.0908038556577102No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC13531.0039847732686271No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA12590.9342326899816715No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA11290.8377670428826903No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG11240.8340568256865757No Hit
TGTAAAANGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC10700.7939864799685373No Hit
TGTAAAANGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG10630.7887921758939768No Hit
TGTAAAANGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT10530.7813717415017475No Hit
TGTAAAANGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA10190.7561422645681678No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA9960.7390752654660404No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG9880.7331389179522569No Hit
TGTAAAANGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC9740.7227503098031359No Hit
TGTAAAANGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC9650.7160719188501294No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG9560.7093935278971231No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT9430.699746963187225No Hit
TGTAAAANGACGGCCAGTAGGAGGGCGGAGGAGGAAAGCAGGTTTGCTCT9340.6930685722342186No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT9330.6923265287949957No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC9080.6737754428144224No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC8950.6641288781045243No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC8760.6500300527592886No Hit
TGTAAAANGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC8640.6411255314886133No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA8580.6366732708532757No Hit
TGTAAAANGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC8490.6299948799002694No Hit
TGTAAAANGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA8390.6225744455080401No Hit
TGTAAAANGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA8290.6151540111158107No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC8290.6151540111158107No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT8240.611443793919696No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC8130.6032813160882439No Hit
TGTAAAANGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT7860.5832461432292246No Hit
TGTAAAANGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG7750.5750836653977724No Hit
TGTAAAACGACGGCCAGTAGGAGGGCGGAGGAGGAAAGCAGGTTTGCTCT7720.5728575350801036No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG7710.5721154916408806No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG7500.556532579417199No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC7490.5557905359779761No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG7470.5543064490995303No Hit
TGTAAAANGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG7460.5535644056603074No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG7360.546143971268078No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT7320.5431757975111864No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG7310.5424337540719634No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC7260.5387235368758487No Hit
TGTAAAANGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG7220.535755363118957No Hit
TGTAAAANGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT7020.5209144943344983No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA6940.514978146820715No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA6840.5075577124284856No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG6820.5060736255500397No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG6800.5045895386715938No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT6790.5038474952323709No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT6660.4942009305224728No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT6630.49197480020480394No Hit
TGTAAAANGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC6560.48678049613024343No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT6320.4689714535888931No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA6210.46080897575744084No Hit
TGTAAAANGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA6020.4467101504122052No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA5950.4415158463376446No Hit
TGTAAAANGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT5870.4355794988238611No Hit
TGTAAAANGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG5730.4251908906747401No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT5670.42073863003940254No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG5650.41925454316095667No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5650.41925454316095667No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC5640.41851249972173377No Hit
TGTAAAANGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC5590.414802282525619No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG5430.4029295874980522No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA5400.4007034571803833No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG5380.3992193703019375No Hit
TGTAAAANGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA5350.39699323998426866No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA5310.394025066227377No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT5240.3888307621528164No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA5150.38215237119981005No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA4850.35989106802312204No Hit
TGTAAAANGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA4770.3539547205093386No Hit
TGTAAAANGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG4740.3517285901916698No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT4650.3450501992386634No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG4590.3405979386033258No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT4590.3405979386033258No Hit
TGTAAAANGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA4580.33985589516410286No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC4480.3324354607718736No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC4410.3272411566973131No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT4400.3264991132580901No Hit
TGTAAAANGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC4400.3264991132580901No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA4350.32278889606197547No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA4200.3116582444736315No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG4050.3005275928852875No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC4020.2983014625676187No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT3920.2908810281753894No Hit
TGTAAAACGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT3890.2886548978577206No Hit
TGTAAAANGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA3820.2834605937831601No Hit
TGTAAAANGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT3810.2827185503439371No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA3790.2812344634654913No Hit
TGTAAAANGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA3710.27529811595170783No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC3680.273071985634039No Hit
TGTAAAANGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC3600.2671356381202556No Hit
TGTAAAANGACGGCCAGTGCTGGAGTGCTGTGGTGTGATGGTCATAGCTG3520.2611992906064721No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATGGTCATAGCTG3340.24784250870045935No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA3290.24413229150434465No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA3270.2426482046258988No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC3160.23448572679444654No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA3150.2337436833552236No Hit
TGTAAAANGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA3130.23225959647677777No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG3110.23077550959833187No Hit
TGTAAAANGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC2940.21816077113154206No Hit
TGTAAAANGACGGCCAGTCTTGCACTACATGGCCATCATGAACCAGCGAA2860.2122244236177586No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2820.20925624986086688No Hit
TGTAAAANGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC2790.20703011954319805No Hit
TGTAAAANGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT2750.20406194578630635No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2750.20406194578630635No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC2710.20109377202941459No Hit
TGTAAAANGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA2680.1988676417117458No Hit
TGTAAAANGACGGCCAGTGCTGGAGTGCAGTGGTGCGATGGTCATAGCTG2520.18699494668417888No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT2470.18328472948806424No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCAGTGGTGCGATGGTCATAGCTG2450.18180064260961837No Hit
TGTAAAANGACGGCCAGTGACCTCATGGGGCGCTTCCGCGTGCTGGGCTG2380.17660633853505786No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC2340.17363816477816613No Hit
TGTAAAACGACGGCCAGTGACCTCATGGGGCGCTTCCGCGTGCTGGGCTG2290.16992794758205143No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT2290.16992794758205143No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2230.16547568694671386No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA2170.16102342631137626No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA2160.16028138287215332No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT2150.15953933943293042No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCATGAACCAGCGAA2090.15508707879759281No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT2070.15360299191914695No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1930.1432143837700259No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT1880.13950416657391124No Hit
TGTAAAANGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT1760.13059964530323606No Hit
TGTAAAANGACGGCCAGTTGATGACCTCTTGGCTAACATACACAACATGA1690.12540534122867553No Hit
TGTAAAANGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT1550.11501673307955447No Hit
TGTAAAANGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGCTCACT1500.11130651588343982No Hit
TGTAAAANGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGATCTATTG1480.10982242900499395No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCAGCTCTCCAGAG1430.1061122118088793No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1390.10314403805198756No Hit
TGTAAAACGACGGCCAGTTGATGACCTCTTGGCTAACATACACAACATGA1370.10165995117354171No Hit
TGTAAAANGACGGCCAGTAGCCCTGCTGTGAAATGATGGAAATGCATTGG1360.10091790773431876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)