FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134763
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC13450.9980484257548436No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC13450.9980484257548436No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA11800.87561125828306No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG10580.7850819586978622No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG8970.6656129649829701No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC7830.5810200129115558No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT7470.5543064490995303No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG7380.5476280581465239No Hit
CAGGAAANAGCTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC7270.5394655803150716No Hit
CAGGAAANAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC7260.5387235368758487No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG7200.5342712762405112No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT7090.5261087984090589No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA6730.49939523459703333No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT6710.49791114771858747No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG6470.4801021051772371No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT6240.46303510607510967No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG6230.46229306263588676No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT6010.44596810697298217No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG5990.4444840200945363No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG5750.42667497755318595No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT5560.41257615220795024No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT5040.37398989336835775No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG5030.37324784992913485No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG4860.360633111462345No Hit
CAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4830.35840698114467623No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA4740.3517285901916698No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG4700.3487604164347781No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA4680.34727632955633225No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC4650.3450501992386634No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC4630.34356611236021756No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4570.33911385172488No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT4510.3346615910895424No Hit
CAGGAAANAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG4400.3264991132580901No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4360.32353093950119843No Hit
CAGGAAANAGCTATGANCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC4330.3213048091835296No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT4280.31759459198741496No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC4190.31091620103440853No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA4180.31017415759518563No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT4160.30869007071673976No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT4120.305721896959848No Hit
CAGGAAANAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT4100.30423781008140216No Hit
CAGGAAANAGCTATGANNTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA4050.3005275928852875No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC3920.2908810281753894No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3840.28494468066160594No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3820.2834605937831601No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA3800.2819765069047142No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG3750.2782662897085995No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG3710.27529811595170783No Hit
CAGGAAANAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT3660.2715878987555932No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA3650.27084585531637023No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG3620.2686197249987014No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCAG3580.2656515512418097No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3560.2641674643633638No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG3560.2641674643633638No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3440.25526294309268865No Hit
CAGGAAANAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC3430.2545208996534657No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA3410.2530368127750198No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3340.24784250870045935No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT3250.24116411774745294No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG3240.24042207430823No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT3230.23968003086900705No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG3180.2359698136728924No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3170.23522777023366948No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT3140.2330016399160007No Hit
CAGGAAANAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG3110.23077550959833187No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG3080.2285493792806631No Hit
CAGGAAANAGCTATGANCACACCCGGAGCAGTGCAAATCAACTCCCCCAG3020.22409711864532547No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG3010.2233550752061026No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG2840.2107403367393127No Hit
CAGGAAANAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG2780.20628807610397512No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC2720.20183581546863752No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT2680.1988676417117458No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG2610.19367333763718528No Hit
CAGGAAANAGCTATGANCACAAACGCAAATGGCTTTTAGCATTTCACAGG2590.1921892507587394No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2570.19070516388029354No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2550.18922107700184768No Hit
CAGGAAANAGCTATGANCCCGTAGCCACGCCCCTATTGTCTGACCACGCT2520.18699494668417888No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2490.1847688163665101No Hit
CAGGAAANAGCTATGANCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT2480.18402677292728717No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2480.18402677292728717No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2480.18402677292728717No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT2470.18328472948806424No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCTGAATAGTTGT2470.18328472948806424No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2460.1825426860488413No Hit
CAGGAAANAGCTATGANNATCACACCACAGCACTCCAGCACTGGCCGTCG2450.18180064260961837No Hit
CAGGAAANAGCTATGANNTGACTTTTGCAAGTCACAGAGAACTGTTGTAT2430.1803165557311725No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2420.17957451229194957No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2390.17734838197428077No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2380.17660633853505786No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2380.17660633853505786No Hit
CAGGAAANAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA2360.17512225165661197No Hit
CAGGAAANAGCTATGANNTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT2340.17363816477816613No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2340.17363816477816613No Hit
CAGGAAANAGCTATGANNGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG2330.17289612133894317No Hit
CAGGAAACAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC2330.17289612133894317No Hit
CAGGAAANAGCTATGACCTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA2320.17215407789972026No Hit
CAGGAAANAGCTATGANCTGGGCCTGGACACTTTGACGGCGGGGCACGTC2310.17141203446049733No Hit
CAGGAAACAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT2300.1706699910212744No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2280.16918590414282852No Hit
CAGGAAANAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2280.16918590414282852No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT2260.16770181726438266No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2250.16695977382515972No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2160.16028138287215332No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTATTCCCAACCA2110.15657116567603868No Hit
CAGGAAANAGCTATGANCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2100.15582912223681575No Hit
CAGGAAANAGCTATGANCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2040.15137686160147815No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2010.14915073128380935No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1980.14692460096614054No Hit
CAGGAAANAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1960.1454405140876947No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1940.14395642720924884No Hit
CAGGAAANAGCTATGANCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1940.14395642720924884No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1930.1432143837700259No Hit
CAGGAAANAGCTATGANCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1930.1432143837700259No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1930.1432143837700259No Hit
CAGGAAANAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG1930.1432143837700259No Hit
CAGGAAANAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG1920.14247234033080297No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1910.14173029689158004No Hit
CAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA1910.14173029689158004No Hit
CAGGAAANAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG1890.14024621001313417No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1870.1387621231346883No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1860.13802007969546537No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1860.13802007969546537No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1830.1357939493777966No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1820.13505190593857364No Hit
CAGGAAANAGCTATGANNTCCCACTTGCTCTTTGAAGGCAATTCCATTCT1800.1335678190601278No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG1800.1335678190601278No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1800.1335678190601278No Hit
CAGGAAANAGCTATGANNCCACCCTCATGCCTAACCCCTATTTTCCAAAG1770.13134168874245897No Hit
CAGGAAANAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1740.12911555842479017No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCACTGGCCGTCG1740.12911555842479017No Hit
CAGGAAANAGCTATGANCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG1720.12763147154634433No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1720.12763147154634433No Hit
CAGGAAANAGCTATGANCTGGGGATGAGGACAGCAGGGCAGTCACCATCT1720.12763147154634433No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA1690.12540534122867553No Hit
CAGGAAANAGCTATGANCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1670.12392125435022967No Hit
CAGGAAANAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1660.12317921091100674No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT1640.12169512403256086No Hit
CAGGAAANAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1640.12169512403256086No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1620.120211037154115No Hit
CAGGAAANAGCTATGANCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1610.11946899371489206No Hit
CAGGAAANAGCTATGANNACCCAGTTAGAGCCATGGTTCCACTAGACTCT1600.11872695027566914No Hit
CAGGAAANAGCTATGACCGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG1590.1179849068364462No Hit
CAGGAAANAGCTATGANCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA1590.1179849068364462No Hit
CAGGAAANAGCTATGANNCTCACCAAGAGTGCCCCACGTGGTGGGCCACA1590.1179849068364462No Hit
CAGGAAANAGCTATGANCTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA1590.1179849068364462No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1580.11724286339722327No Hit
CAGGAAANAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG1570.11650081995800035No Hit
CAGGAAANAGCTATGANNAGGACCACCCCTCCATCCTGGCCAGAGGGGTC1560.11575877651877742No Hit
CAGGAAANAGCTATGACCTGACTTTTGCAAGTCACAGAGAACTGTTGTAT1530.11353264620110862No Hit
CAGGAAANAGCTATGANNCTGGGCCCCAAAGCAAAGGACTCTTCCCAGTG1530.11353264620110862No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA1510.11204855932266274No Hit
CAGGAAANAGCTATGANNTTGCCAGTGCTGCCTTCAGCCATTTGTGAAAT1490.11056447244421688No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG1490.11056447244421688No Hit
CAGGAAANAGCTATGACCATCACACCACAGCACTCCAGCACTGGCCGTCG1490.11056447244421688No Hit
CAGGAAANAGCTATGANCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1480.10982242900499395No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1480.10982242900499395No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA1460.10833834212654808No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA1440.10685425524810223No Hit
CAGGAAANAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT1410.10462812493043344No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1410.10462812493043344No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1400.1038860814912105No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1380.10240199461276463No Hit
CAGGAAANAGCTATGANCTGACAGCACAAGGAACACTGACCTACAGATAA1360.10091790773431876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)