FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91102
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC9931.0899870474852362No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG8850.9714386072753618No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC8450.9275317775680006No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8310.9121643871704244No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG8260.9066760334570042No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC8260.9066760334570042No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA8050.8836249478606397No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT8000.8781365941472197No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA6740.7398300805690325No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT6470.7101929705165638No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC6230.6838488726921473No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6030.6618954578384668No Hit
CAGGAAANAGCTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC5550.6092072621896336No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA5540.6081095914469495No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT5350.5872538473359531No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5260.5773748106517969No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5110.5609097495115365No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA5040.5532260543127484No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC4900.537858663915172No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA4830.5301749687163838No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4810.5279796272310158No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT4790.5257842857456477No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG4640.5093192246053874No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG4180.4588263704419222No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA3970.4357752848455577No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG3920.43028693113213756No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3900.4280915896467696No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3890.42699391890408556No Hit
CAGGAAANAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT3770.4138218699918772No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3770.4138218699918772No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3770.4138218699918772No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT3670.402845162565037No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3640.39955215033698493No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3640.39955215033698493No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3590.39406379662356483No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3520.38638010142477663No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG3450.37869640622598844No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3380.37101271102720024No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3290.36113367434304405No Hit
CAGGAAANAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT3290.36113367434304405No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG3250.35674299137230797No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT3210.35235230840157183No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3190.35015696691620385No Hit
CAGGAAANAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG3120.34247327171741565No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3120.34247327171741565No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3080.33808258874667957No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA3080.33808258874667957No Hit
CAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3070.3369849180039955No Hit
CAGGAAANAGCTATGANNTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT3060.33588724726131153No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA3020.3314965642905754No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3000.32930122280520735No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3000.32930122280520735No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2930.3216175276064192No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2900.3183245153783671No Hit
CAGGAAANAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC2850.312836161664947No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA2810.3084454786942109No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2740.3007617834954227No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA2710.29746877126737065No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2650.2908827468112665No Hit
CAGGAAANAGCTATGANCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2610.2864920638405304No Hit
CAGGAAANAGCTATGANCCAGGGCTCCAAGAATGTTCCACACATCTTTCT2500.2744176856710061No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG2490.2733200149283221No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2440.267831661214902No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2420.26563631972953394No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT2420.26563631972953394No Hit
CAGGAAANAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG2360.25905029527342976No Hit
CAGGAAANAGCTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2330.2557572830453777No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2260.24807358784658956No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2230.24478057561853747No Hit
CAGGAAANAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2180.23929222190511734No Hit
CAGGAAANAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG2160.2370968804197493No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCTGAATAGTTGT2150.23599920967706528No Hit
CAGGAAANAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA2130.23380386819169724No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2120.2327061974490132No Hit
CAGGAAANAGCTATGANCCCGTAGCCACGCCCCTATTGTCTGACCACGCT2100.23051085596364515No Hit
CAGGAAANAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT2080.2283155144782771No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA2030.22282716076485698No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT2030.22282716076485698No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2020.22172949002217296No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2010.2206318192794889No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2010.2206318192794889No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2010.2206318192794889No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2010.2206318192794889No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2000.21953414853680492No Hit
CAGGAAANAGCTATGANCTGGGCCTGGACACTTTGACGGCGGGGCACGTC1990.21843647779412087No Hit
CAGGAAACAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC1870.2052644288819126No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1850.20306908739654456No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG1820.19977607516849247No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1820.19977607516849247No Hit
CAGGAAANAGCTATGANNGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG1810.19867840442580842No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1810.19867840442580842No Hit
CAGGAAANAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1800.1975807336831244No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1800.1975807336831244No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG1780.19538539219775636No Hit
CAGGAAANAGCTATGANNCTGGTCCTCATCCTTGGGGCACTCAGCGTGTG1750.1920923799697043No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1740.19099470922702028No Hit
CAGGAAANAGCTATGANNTTGCCAGTGCTGCCTTCAGCCATTTGTGAAAT1690.18550635551360012No Hit
CAGGAAANAGCTATGANCACAAACGCAAATGGCTTTTAGCATTTCACAGG1680.1844086847709161No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1600.1756273188294439No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1580.17343197734407587No Hit
CAGGAAANAGCTATGANCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1580.17343197734407587No Hit
CAGGAAANAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1510.1657482821452877No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1500.16465061140260367No Hit
CAGGAAANAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG1500.16465061140260367No Hit
CAGGAAANAGCTATGACCCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1420.15586924546113148No Hit
CAGGAAANAGCTATGANNCCACCCTCATGCCTAACCCCTATTTTCCAAAG1380.15147856249039537No Hit
CAGGAAANAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC1360.14928322100502733No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA1360.14928322100502733No Hit
CAGGAAANAGCTATGANCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1360.14928322100502733No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1350.1481855502623433No Hit
CAGGAAANAGCTATGANCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1320.14489253803429122No Hit
CAGGAAANAGCTATGANCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA1300.14269719654892318No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT1270.13940418432087112No Hit
CAGGAAANAGCTATGANCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1240.13611117209281903No Hit
CAGGAAANAGCTATGANNAGGACCACCCCTCCATCCTGGCCAGAGGGGTC1240.13611117209281903No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG1220.133915830607451No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1220.133915830607451No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1200.13172048912208292No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1190.1306228183793989No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1140.1251344646659788No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1140.1251344646659788No Hit
CAGGAAANAGCTATGANNCCTGACCCCTTCTTCTCCAGGAAACTCTGTTC1130.12403679392329478No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1120.12293912318061076No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA1120.12293912318061076No Hit
CAGGAAANAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG1120.12293912318061076No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1110.12184145243792671No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1110.12184145243792671No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACCAGGTCCCGGGA1110.12184145243792671No Hit
CAGGAAANAGCTATGANCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1100.12074378169524269No Hit
CAGGAAANAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1090.11964611095255867No Hit
CAGGAAANAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1090.11964611095255867No Hit
CAGGAAANAGCTATGACCGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG1080.11854844020987465No Hit
CAGGAAANAGCTATGANNAGGTCCAGATGGCTGGTGACTCACACCTGTAA1080.11854844020987465No Hit
CAGGAAANAGCTATGANCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT1080.11854844020987465No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTTTCAGAGATGGT1060.1163530987245066No Hit
CAGGAAANAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA1040.11415775723913855No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1030.11306008649645452No Hit
CAGGAAANAGCTATGACCCTGGTCCTCATCCTTGGGGCACTCAGCGTGTG1030.11306008649645452No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC1010.11086474501108648No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1010.11086474501108648No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1000.10976707426840246No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCAGGAAACAGCT970.10647406204035037No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA970.10647406204035037No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA970.10647406204035037No Hit
CAGGAAANAGCTATGANNCCTGACCCCTTCTTCTCCAGGAAACTCTCTTC950.10427872055498233No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT950.10427872055498233No Hit
CAGGAAACAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG930.10208337906961427No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT930.10208337906961427No Hit
CAGGAAACAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC930.10208337906961427No Hit
NAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG930.10208337906961427No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT930.10208337906961427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)