FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141337
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG16471.1652999568407425No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA15511.0973771906860907No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC14431.0209640787621077No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT14171.0025683295952228No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA9560.6763975462900726No Hit
CAGGAAANAGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT9350.6615394411937426No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA8650.6120124242059758No Hit
CAGGAAANAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT8630.6105973665777539No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG8120.5745133970580952No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC8080.5716832818016514No Hit
CAGGAAANAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC7930.561070349589987No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG7450.5271089665126613No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG7360.5207412071856626No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA7140.5051755732752217No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC6760.47828947833900537No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT6700.4740443054543396No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC6620.468384074941452No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA6430.45494102747334386No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT6220.4400829223770138No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG5920.41885705795368516No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5860.4146118850690194No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA5810.4110742409984647No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA5750.40682906811379893No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC5660.40046130878680036No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT5570.39409354945980174No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG5550.3926784918315798No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT5510.38984837657513605No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC5470.3870182613186922No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG5470.3870182613186922No Hit
CAGGAAANAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT5310.37569780029291694No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG5280.37357521385058406No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC5210.36862251215180736No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC5160.3650848680812526No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC5140.3636698104530307No Hit
CAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4930.34881170535670064No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG4860.343859003657924No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA4860.343859003657924No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC4810.3403213595873692No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC4680.3311234850039268No Hit
CAGGAAANAGCTATGANCGGGGAGACTGGGCCATACGATAGGCTTAATCC4670.33041595618981584No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT4550.3219256104204844No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4400.31131267820882No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA4350.3077750341382653No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4330.3063599765100434No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT4230.29928468836893385No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA4150.2936244578560462No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4000.28301152564438187No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA4000.28301152564438187No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC3970.280888939202049No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3710.2624931900351642No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3690.2610781324069423No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT3650.25824801715049844No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG3570.25258778663761083No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC3540.25046520019527796No Hit
CAGGAAANAGCTATGANNGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG3490.24692755612472317No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT3400.2405597967977246No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3400.2405597967977246No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC3390.23985226798361364No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT3380.23914473916950268No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC3260.23065439340017121No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3260.23065439340017121No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3250.22994686458606026No Hit
CAGGAAANAGCTATGANCCCCGTGACAGCTCCTGCAATTACAAAATGGCT3230.22853180695783837No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCAGTAATGGACTGG3170.22428663407317262No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG3140.22216404763083977No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC3090.21862640356028498No Hit
CAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA3060.21650381711795214No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3060.21650381711795214No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG3050.21579628830384118No Hit
CAGGAAANAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT3000.2122586442332864No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC2990.21155111541917546No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2980.2108435866050645No Hit
CAGGAAANAGCTATGANNAGCAGCTTTCAAACCTAGACACTGGCAGGTGG2970.21013605779095354No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2950.20872100016273162No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTGGAAACTGGGAA2930.2073059425345097No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2920.20659841372039878No Hit
CAGGAAANAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2850.20164571202162207No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2830.20023065439340018No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2780.1966930103228454No Hit
CAGGAAANAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2770.19598548150873443No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTTGGGAAAGACTG2770.19598548150873443No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2750.19457042388051254No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2750.19457042388051254No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2680.18961772218173584No Hit
CAGGAAANAGCTATGANNTTCCTGATGGGATTCAGCCATCAACAGTAGGA2670.1889101933676249No Hit
CAGGAAANAGCTATGANNCCACCCTCATGCCTAACCCCTATTTTCCAAAG2630.18608007811118107No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2610.18466502048295916No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA2570.18183490522651535No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT2550.18041984759829344No Hit
CAGGAAANAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT2460.17405208827129484No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2440.17263703064307295No Hit
CAGGAAANAGCTATGANCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2440.17263703064307295No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2400.16980691538662912No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2400.16980691538662912No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA2390.16909938657251816No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCGCATTGTAGTCAA2340.1655617425019634No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2330.16485421368785244No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT2320.16414668487374148No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA2280.16131656961729768No Hit
CAGGAAANAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2270.16060904080318672No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2250.1591939831749648No Hit
CAGGAAANAGCTATGANCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2250.1591939831749648No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2240.15848645436085385No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG2240.15848645436085385No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2220.15707139673263193No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2200.15565633910441No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC2190.15494881029029908No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2180.15424128147618812No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2180.15424128147618812No Hit
CAGGAAANAGCTATGANCTGACAGCACAAGGAACACTGACCTACAGATAA2160.1528262238479662No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2150.15211869503385525No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2140.1514111662197443No Hit
CAGGAAANAGCTATGANCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC2130.15070363740563333No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2080.14716599333507857No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC2070.1464584645209676No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2070.1464584645209676No Hit
CAGGAAANAGCTATGANCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC2050.1450434068927457No Hit
CAGGAAANAGCTATGANCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2040.14433587807863474No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2030.1436283492645238No Hit
CAGGAAANAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT2030.1436283492645238No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC2020.14292082045041285No Hit
CAGGAAANAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG2000.14150576282219093No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA2000.14150576282219093No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1990.14079823400807998No Hit
CAGGAAANAGCTATGANCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1980.14009070519396902No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1980.14009070519396902No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1970.13938317637985806No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1970.13938317637985806No Hit
CAGGAAANAGCTATGACCAAGGACAAGGTGAGGCAGCAGTAATGGACTGG1930.13655306112341425No Hit
CAGGAAANAGCTATGACCAGCAGCTTTCAAACCTAGACACTGGCAGGTGG1880.13301541705285946No Hit
CAGGAAANAGCTATGANNACCCCTTAAGCCAGAGCCGACTGGCCGTCGTT1870.13230788823874853No Hit
CAGGAAANAGCTATGANCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1860.13160035942463758No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT1840.13018530179641566No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1840.13018530179641566No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1830.1294777729823047No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1810.12806271535408278No Hit
CAGGAAANAGCTATGANCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA1800.12735518653997185No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1770.12523260009763898No Hit
CAGGAAANAGCTATGACCAGGCTTTGTGTGTGGGGACTGGAAACTGGGAA1740.1231100136553061No Hit
CAGGAAANAGCTATGANCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1720.1216949560270842No Hit
CAGGAAANAGCTATGANNGCATCACTGAGGGAGGATCCTCGGGTCTTACA1720.1216949560270842No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1720.1216949560270842No Hit
CAGGAAANAGCTATGANCGTCAACAGCATACCTGTACACCTGAAATAACT1690.11957236958475134No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1690.11957236958475134No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1690.11957236958475134No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1690.11957236958475134No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGCAGAGGGTTGGGAAAGACTG1680.11886484077064038No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA1640.11603472551419657No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1610.1139121390718637No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1600.11320461025775275No Hit
CAGGAAANAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1590.11249708144364179No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1560.11037449500130893No Hit
CAGGAAANAGCTATGANCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1540.10895943737308703No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGTAGAGTGTTGGGAAAGACTG1520.10754437974486511No Hit
CAGGAAANAGCTATGACCTTCCTGATGGGATTCAGCCATCAACAGTAGGA1510.10683685093075415No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1490.10542179330253225No Hit
CAGGAAANAGCTATGANCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1470.10400673567431033No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1460.10329920686019939No Hit
CAGGAAANAGCTATGANCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1440.10188414923197747No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1440.10188414923197747No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGCAGAGGGTTGGGAAAGACTG1420.10046909160375556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)