FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130532
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA50823.893298195078601No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT46383.5531517175864926No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC33592.57331535562161No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA33372.55646125088101No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC31202.3902184904850916No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA28722.200226764318328No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA28052.1488983544265006No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG26902.0607973523733647No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC26602.0378144822725464No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT22841.7497625103422916No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC21621.6562988385989643No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC20011.5329574357245734No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT19831.5191677136640824No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT18421.4111482241902369No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG17891.3705451536787916No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG17641.3513927619281096No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA17511.341433518217755No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA17471.338369135537646No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC16251.2449054637943187No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG14831.1361198786504458No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC14371.1008794778291913No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT13871.0625746943278276No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA13521.035761345876873No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC13341.0219716238163823No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG13020.9974565623755094No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC12740.9760058836147458No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG12400.9499586308338186No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC12260.9392332914534366No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATGAGTTAGCCAGC11710.8970980296019367No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG11320.867220298470873No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG10930.8373425673398095No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT10410.7975055924983913No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTTCCTGCAACAC9150.7009775380749549No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC9000.6894861030245457No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA8770.6718659026139184No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG8600.6588422762234548No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT8270.6335611191125547No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC7990.6121104403517912No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT7650.5860631875708638No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA7610.5829988048907547No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT7590.5814666135507002No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT7520.5761039438605093No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT6930.5309042993289003No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA6280.4811080807771274No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTCTCCATCTCCA6060.46425397603652746No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG5930.45429473232617296No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCGCTCTCCTCCC5820.4458676799558729No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCCACAGAGAGCAC5810.44510158428584556No Hit
CAGGAAACAGCTATGACCTCCAGCTGCTCCATCTTGTCTACCTCCCGGCC5660.4336101492354365No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC5540.4244170011951092No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC5330.4083289921245365No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCGCTCAGCTCC5120.39224098305396377No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT4860.3723224956332547No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTGCCTCATGTCC4810.3684920172831183No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG4800.36772592161309103No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT4290.32865504244170013No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC4280.32788894677167285No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC4270.3271228511016456No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA4160.31869579873134557No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAGAAGTCTTTTAA4030.308736555020991No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG3990.30567217234088195No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACACACACTTCTAA3990.30567217234088195No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATTCTCCTCACACC3960.3033738853308001No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGAGGAGCCGGT3950.30260778966077284No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCTGCCAGGAGCAGG3620.27732663254987283No Hit
CAGGAAACAGCTATGACCAACACCCTTGGCCTCCCTCACTGGCCGTCGTT3610.27656053687984555No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA3230.2474489014188092No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA2850.2183372659577728No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGTTGCAGCCAAGG2810.21527288327766372No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG2790.2137406919376092No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA2710.20761192657739097No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC2560.19612049152698188No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTCACATCTATAAT2490.19075782183679096No Hit
CAGGAAACAGCTATGACCCTGGGCCCCAAAGCAAAGGACTCTTCCCAGTG2440.1869273434866546No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTATTCCCAACCA2390.18309686513651827No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCCCCTGCCTACC2360.18079857812643643No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAATG2350.18003248245640915No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC2330.1785002911163546No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCCGT2290.1754359084362455No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGAAGAATCGC2250.17237152575613643No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAGGGTTCGAGAAC2170.16624276039591823No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCTCCAGAACAC2140.16394447338583643No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCACTAGACTCT2090.16011399503570006No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGAAGAATCAC2040.1562835166855637No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1950.14938865565531823No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTTTCAGGGAAGC1890.14479208163515458No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATACCTGCTCTGGG1790.1371311249348819No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC1690.12947016823460913No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTA1670.12793797689455458No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCACAGGTCCCACCC1540.11797873318420003No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT1540.11797873318420003No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGTGGGAGGATTGC1490.11414825483406368No Hit
CAGGAAACAGCTATGACCTCTGCAGCTAGCTGGGAACTGGCCGTCGTTTT1480.1133821591640364No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1440.11031777648392732No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGAGGCAGGAGAA1380.10572120246376368No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT1370.1049551067937364No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)