FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161330
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT47612.9510940308684064No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC47332.9337383003781072No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT28991.7969379532634973No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG22591.4002355420566541No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT19111.1845286059629332No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT18421.1417591272546954No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC18321.1355606520795885No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG16100.9979545031922147No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC13950.8646872869274158No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT10590.6564185210438233No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC10080.6248062976507779No Hit
CAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT10060.6235666026157566No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT9680.6000123969503502No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT9680.6000123969503502No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT9350.5795574288724974No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC9100.5640612409347301No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG8900.5516642905845163No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT8760.5429864253393665No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCAGAGAGTGATAT8090.5014566416661501No Hit
CAGGAAANNGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG7860.48720014876340423No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA7740.4797619785532759No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG7720.4785222835182545No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT7240.4487696026777413No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT7070.4382321948800595No Hit
CAGGAAANNGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG6310.39112378354924693No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA6310.39112378354924693No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG6110.37872683319903305No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC5910.3663298828488192No Hit
NAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5610.3477344573234984No Hit
NAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5510.3415359821483915No Hit
CAGGAAANAGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT5410.33533750697328457No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5400.33471765945577386No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC5360.3322382693857311No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5170.320461166553028No Hit
CAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5160.31984131903551727No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC5060.3136428438604103No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC5040.31240314882538894No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4970.3080642162028141No Hit
CAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4920.3049649786152606No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG4900.30372528358023926No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA4480.2776916878447902No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4470.2770718403272795No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA4430.2745924502572367No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA4330.2683939750821298No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG4160.25785656728444806No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA3970.24607946445174486No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT3950.24483976941672347No Hit
CAGGAAANNGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC3820.23678175168908447No Hit
CAGGAAANNGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3370.20888861340110335No Hit
CAGGAAANNGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT3330.20640922333106057No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3300.20454968077852848No Hit
CAGGAAANNGCTATGANNAATGCAAGGGACCACCAGGCAGAGAGTGATAT3290.2039298332610178No Hit
NAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3260.2020702907084857No Hit
CAGGAAANNGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC3160.1958718155333788No Hit
NAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2990.18533440773569704No Hit
CAGGAAANNGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2970.18409471270067562No Hit
CAGGAAANNGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2910.1803756275956115No Hit
CAGGANANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2890.1791359325605901No Hit
CAGGAAACNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2850.17665654249054732No Hit
NAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2840.17603669497303664No Hit
CAGGAANNNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2820.17479699993801526No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG2780.17231760986797248No Hit
CAGGAAANNGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2710.16797867724539764No Hit
NAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2710.16797867724539764No Hit
CAGGAAACAGCNATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2640.1636397446228228No Hit
NAGGANANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2620.1624000495878014No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2580.15992065951775863No Hit
CAGGAAANNGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT2560.15868096448273725No Hit
CAGGAAACAGCNATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2500.15496187937767308No Hit
CAGGAAACNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2470.15310233682514102No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG2440.15124279427260895No Hit
CAGGAAACAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2440.15124279427260895No Hit
CAGGAAANNGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2430.15062294675509824No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2420.15000309923758756No Hit
NAGGAAANNGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2360.1462840141325234No Hit
CAGGAANNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2300.14256492902745926No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2290.14194508150994856No Hit
NAGGANANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2270.14070538647492717No Hit
CAGGANANNGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2260.14008553895741646No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2250.13946569143990578No Hit
NAGGANANNGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2250.13946569143990578No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2170.13450691129982026No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCTGACCAACACCC2130.13202752122977748No Hit
CAGGAAACAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2110.13078782619475607No Hit
NAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2050.12706874108969193No Hit
CAGGAAANAGCTATGANNTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT2040.12644889357218123No Hit
CAGGAAANNGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG2030.12582904605467057No Hit
CAGGAAANNGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT1980.12272980846711708No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1960.12149011343209572No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCACGGTGGCTCAT1910.11839087584454223No Hit
CAGGAAANAGCTATGANNTCCCTGCTTGATAATGACCCCTCTGAATTCTT1910.11839087584454223No Hit
CAGGANANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1880.11653133329201017No Hit
NAGGAAANNGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG1860.11529163825698878No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGCAGAAGGG1850.11467179073947809No Hit
CAGGAAANNGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1800.11157255315192463No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACCAGGTCCCGGGA1790.11095270563441394No Hit
NAGGANANNGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG1780.11033285811690324No Hit
CAGGAAACAGCNATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1760.10909316308188184No Hit
NAGGAAANAGCTATGANCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1750.10847331556437118No Hit
CAGGANANNGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG1720.10661377301183908No Hit
CAGGAANNNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1690.104754230459307No Hit
CAGGAAANNGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1660.10289468790677493No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1650.10227484038926424No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCGCAGTGGCTGAT1630.10103514535424285No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)