FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121094
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT35872.9621616265050292No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC25172.078550547508547No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC24412.015789386757395No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG23821.96706690670058No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC21661.7886930814078321No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG21651.7878672766611061No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC21431.7696995722331414No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCACAGACCCCACA20121.6615191504120768No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA18171.500487224800568No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCGGACGAGGA16661.3757907080449898No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT16651.3749649032982643No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG16051.3254166184947231No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC16021.322939204254546No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT15871.310552133053661No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT15831.3072489140667582No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT15711.2973392571060498No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA15221.2568748245164914No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG15021.2403587295819776No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA14841.2254942441409153No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG14781.2205394156605611No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCAGGAAACAGCT14041.1594298644028607No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA13641.1263976745338333No Hit
CAGGAAACAGCTATGACCGAGGCGCAGGTCACAGTGAGACCAATACAGAG11490.9488496539878112No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG11140.9199464878524123No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGA11020.9100368308917038No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT10910.9009529786777215No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG10480.8654433745685169No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC10350.8547079128610832No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC10010.8266305514724098No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC9910.818372504005153No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC9740.8043338233108164No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCAAAATTATCCT9250.7638693907212579No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC9110.7523081242670984No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG8850.7308372008522305No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTATGACCGCCCACC8850.7308372008522305No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGATGCCACG8340.6887211587692207No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA8210.6779856970617867No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT8200.677159892315061No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT7990.6598179926338216No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC7720.6375212644722281No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG7540.6226567790311659No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTGCCATGCTCACC7500.6193535600442631No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGACTATGCTGAA7490.6185277552975375No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGCATCCTGG7420.6127471220704577No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT7140.5896245891621384No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA6790.5607214230267396No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG6510.5375988901184203No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA6430.5309924521446149No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATCCGAGTGAGGAG6270.517779576197004No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA6220.5136505524633755No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA6060.5004376765157647No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC5500.4541926106991263No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTGGGCCA5440.4492377822187722No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCACGCCCGGCCT5410.44676036797859514No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT5410.44676036797859514No Hit
CAGGAAACAGCTATGACCTGGAGCCAATTTCATGAGGGCAGGAAACAGCT5250.4335474920309842No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT5240.43272168728425847No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTTGAGCTCCTCA5240.43272168728425847No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTAGGCTAGCA4960.40959915437593936No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTAGCCCACCGCAAA4900.40464432589558524No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG4790.39556047368160274No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4770.39390886418815135No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGGGCGGA4730.39060564520124863No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTCCTCAGCCACC4730.39060564520124863No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTAATCTCGAG4720.38977984045452296No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT4710.38895403570779724No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT4690.3873024262143459No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC4660.38482501197416885No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGACCCTGGCAG4580.37821857400036335No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4560.37656696450691196No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT4550.3757411597601863No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT4490.3707863312798322No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4410.36417989330602674No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCATCTGCCCCTT4280.35344443159859285No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGAAGGGCCACCCCA4170.34436057938461034No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC4050.33445092242390206No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTCAGGAAAACAGC4040.3336251176771764No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATCCAGCAGCAGAG3990.329496093943548No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3890.3212380464762912No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT3850.3179348274893884No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG3810.31463160850248567No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3630.29976712306142334No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC3580.29563809932779495No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG3540.29233488034089217No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGACAAGTGCATGG3290.2716897616727501No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGGCCGGCCCCTCA3270.2700381521792987No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGGCCCCAGCCACAG3260.26921234743257305No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTACCATGCTCACC3250.2683865426858474No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCTGA3130.2584768857251391No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGCCGGGACGCCCC2940.24278659553735116No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA2850.23535435281682No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCCTGGCTTGTCC2810.23205113382991724No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCAGCCACCACCACGTACCACT2810.23205113382991724No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTTGGAGTCCCAC2760.22792211009628882No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATGAGTTAGCCAGC2760.22792211009628882No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTAGCTATGACCGCC2600.21470923414867787No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTACTGACCATCT2560.21140601516177515No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAACCAGTGAC2530.2089286009215981No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG2510.20727699142814673No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTGTCCTCCTC2500.20645118668142104No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTAGGCCA2500.20645118668142104No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAACCCCATCTCTA2500.20645118668142104No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTACATGGCAAAAC2480.20479957718796968No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT2470.203973772441244No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTACATCAAATGC2460.20314796769451832No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCTCCCTTAGGAGT2340.1932383107338101No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2240.18498026326655326No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTCAGGGAAAG2240.18498026326655326No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACAGGAAACAGCT2190.1808512395329248No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCTCGTTAGCTGC2160.17837382529274778No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC2140.17672221579929642No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCTGCTTGTGT2080.1717673873189423No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCACTGCATTC1950.16103192561150842No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1950.16103192561150842No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1940.16020612086478273No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCCACAGAGAGCAC1910.15772870662460567No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGAAGGGCCACCCAC1830.15112226865080022No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAACTCCTGAGATC1790.14781904966389747No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGGACCCTTGAGCT1780.14699324491717178No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAAGGCACTACG1760.14534163542372042No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT1740.14369002593026906No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG1630.13460617371628653No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCCTCGTGGGTC1590.1313029547293838No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCAAAGCCACTGCAGC1550.12799973574248105No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTACAGAGTTAA1540.12717393099575536No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCCAGCTGGA1500.12387071200885263No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCTGAAGAGAGACA1460.1205674930219499No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC1420.11726427403504715No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1410.11643846928832147No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATACCTGTAATCC1400.11561266454159579No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG1400.11561266454159579No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATCCCAGCAGCAGA1360.11230944555469305No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGTGCCACTGCACTGCA1350.11148364080796737No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCGGGCAG1350.11148364080796737No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT1320.10900622656779033No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTAGGAAACAGCTAT1290.10652881232761326No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATAAACCAGTTACC1270.10487720283416188No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAATAGGTCAGTAC1250.10322559334071052No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACAGCCTGGCCAA1230.10157398384725916No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTTCCACACGCCTG1230.10157398384725916No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)