FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137935
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG11830.8576503425526516No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT10970.7953021350636169No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA10570.7663029687896473No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT10420.7554282814369087No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC9860.7148294486533512No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC9750.7068546779280096No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT9280.6727806575560953No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG8160.5915829919889803No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA8160.5915829919889803No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT7940.575633450538297No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC7510.5444593467937797No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7500.5437343676369304No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA7150.518360097147207No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC7080.5132852430492624No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT6580.4770362852068003No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC6470.4690615144814587No Hit
CAGGAAANAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC6310.45746184797187084No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC6270.45456193134447387No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT6160.4465871606191322No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG6020.43643745242324283No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA5990.4342625149526951No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5850.42411280675680574No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC5740.4161380360314641No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5720.4146880777177656No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT5700.4132381194040672No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA5490.39801355711023306No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG5290.38351397397324827No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG5130.3719143074636604No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA5060.3668394533657157No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4970.36031464095407256No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA4840.35088991191503244No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4780.34654003697393704No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT4760.3450900786602385No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT4670.33856526624859534No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT4560.3305904955232537No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4530.328415558052706No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4520.32769057889585673No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG4510.32696559973900746No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC4460.32334070395476133No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA4370.3168158915431181No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT4140.30014137093558557No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG4130.29941639177873636No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT4070.2950665168376409No Hit
CAGGAAANAGCTATGANNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC3920.2841918294849023No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3890.2820168920143546No Hit
CAGGAAANAGCTATGANCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC3880.2812919128575053No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG3870.2805669337006561No Hit
CAGGAAANAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC3800.27549207960271144No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3790.2747671004458622No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3780.27404212128901295No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3760.27259216297531447No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC3740.271142204661616No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3700.268242288034219No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3690.2675173088773698No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3680.2667923297205205No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT3670.26606735056367126No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3660.26534237140682204No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3650.2646173922499728No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG3630.26316743393627434No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT3560.25809257983832967No Hit
CAGGAAANAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3500.25374270489723416No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3420.2479428716424403No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3400.2464929133287418No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT3360.24359299670134482No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA3230.2341682676623047No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG3230.2341682676623047No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA3220.23344328850545548No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC3190.23126835103490775No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3170.22981839272120927No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA3140.22764345525066154No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3140.22764345525066154No Hit
CAGGAAANAGCTATGANCTGGGGATGAGGACAGCAGGGCAGTCACCATCT3130.2269184760938123No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3090.22401855946641536No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3050.2211186428390184No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3000.2174937470547722No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2940.21314387211367672No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2920.21169391379997826No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2870.20806901801573205No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2840.20589408054518432No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2830.20516910138833505No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG2800.20299416391778738No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2790.2022691847609381No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2780.20154420560408887No Hit
CAGGAAANAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT2700.19574437234929498No Hit
CAGGAAANAGCTATGANCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2690.1950193931924457No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2640.1913944974081995No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2620.18994453909450104No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2600.18849458078080256No Hit
CAGGAAANAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT2600.18849458078080256No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2580.18704462246710407No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2570.18631964331025483No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2490.1805198100554609No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2460.1783448725849132No Hit
CAGGAAANAGCTATGANCGGGGAGACTGGGCCATACGATAGGCTTAATCC2430.17616993511436546No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2430.17616993511436546No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC2430.17616993511436546No Hit
CAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2410.17471997680066698No Hit
CAGGAAANAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2350.17037010185957155No Hit
CAGGAAANAGCTATGANCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2310.16747018523217458No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2260.16384528944792837No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2240.1623953311342299No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2230.16167035197738067No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2190.1587704353499837No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2160.15659549787943597No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2160.15659549787943597No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2130.15442056040888824No Hit
CAGGAAANAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC2130.15442056040888824No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2120.153695581252039No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2120.153695581252039No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT2110.15297060209518976No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2080.15079566462464206No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2050.14862072715409433No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2040.1478957479972451No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2010.14572081052669736No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1990.14427085221299887No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1960.14209591474245115No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1920.1391959981150542No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1920.1391959981150542No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1900.13774603980135572No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1900.13774603980135572No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC1890.13702106064450648No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1870.135571102330808No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1860.13484612317395875No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1840.13339616486026026No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG1810.13122122738971254No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1780.12904628991916484No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1780.12904628991916484No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1770.1283213107623156No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1750.12687135244861708No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1750.12687135244861708No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1750.12687135244861708No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT1750.12687135244861708No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1740.12614637329176787No Hit
CAGGAAANAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1740.12614637329176787No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1710.12397143582122015No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT1690.12252147750752165No Hit
CAGGAAANAGCTATGANCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1630.11817160256642621No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1610.11672164425272774No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1610.11672164425272774No Hit
CAGGAAANAGCTATGANCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT1610.11672164425272774No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1580.11454670678218001No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1570.11382172762533077No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1550.1123717693116323No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1550.1123717693116323No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1490.10802189437053684No Hit
CAGGAAANAGCTATGANCCTGGAATTTCAACAGAATGGCATTTATATGAT1470.10657193605683836No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT1420.10294704027259216No Hit
CAGGAAANAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA1410.10222206111574292No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1410.10222206111574292No Hit
CAGGAAANAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG1390.10077210280204443No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1390.10077210280204443No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1380.10004712364519519No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1380.10004712364519519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)