FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162978
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC37252.2855845574249285No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT33922.0812625016873443No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG32852.0156094687626553No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT31581.9376848408987717No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA29801.8284676459399427No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC29251.7947207598571588No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA26471.6241455902023587No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC26411.6204641117206005No Hit
TGTAAAACGACGGCCAGTTCCTCCTTTGCCAATATAGGGTTTGATGTAAT25661.5744456306986219No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC23121.418596374970855No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT22721.3940531850924665No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC22461.378100111671514No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA22341.3707371547079974No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC22231.3639877774914404No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC22151.3590791395157629No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG20071.2314545521481428No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT19401.190344709101842No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT19291.1835953318852852No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT17831.094012688829167No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC17731.08787689135957No Hit
TGTAAAACGACGGCCAGTATCTGCACATCGTGCTGCCACACGAGGCTTCA17041.04553988881935No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTCTAGCCTGGGCA16591.0179288002061628No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG16581.017315220459203No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC16571.0167016407122433No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC16421.0074979445078478No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG15350.9418449115831584No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT15180.9314140558848433No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC14950.91730172170477No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA14800.9080980255003743No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC14770.9062572862594952No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG14070.8633067039723153No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT13700.8406042533348059No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT13480.8271054989016922No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC13280.814833903962498No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC13150.8068573672520217No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT12550.770042582434439No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC12440.7632932052178821No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT11290.6927315343175152No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC11090.6804599393783209No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT10680.6553031697529728No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC10540.6467130532955367No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA10470.6424179950668188No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC10420.6393500963320203No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC9830.6031488912613971No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG9710.5957859342978807No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAGGAAATCAGG9470.5810600203708476No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA9010.5528353520107009No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG8990.5516081925167814No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG8880.5448588153002245No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA8810.5405637570715066No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCATGTAAAACGACG8770.5381094380836677No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC8690.53320080010799No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT8520.522769944409675No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC7690.4718428254120188No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA7660.4700020861711397No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC7000.4295058228717986No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGTT6910.4239836051491612No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG6650.4080305317282087No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA6260.38410092159677994No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG6230.3822601823559008No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA6160.3779651241271828No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG6130.3761243848863037No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCCTGAGGGAGGAT6120.37551080513934393No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG6110.37489722539238424No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT6060.3718293266575857No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG5920.3632392102001497No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT5910.36262563045319No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG5870.36017131146535114No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA5720.35096761526095543No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTGCACTCCAGCTG5680.3485132962731166No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG5530.33930960006872096No Hit
TGTAAAACGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT5330.3270380051295267No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTGCCTGAAAGAAG5140.3153799899372921No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT5070.31108493170857415No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCGGAGGATCCTCC5010.3074034532268159No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGCACGCAGGCT4900.30065407601025906No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG4830.29635901778154106No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGCTTGACACGG4670.28654174183018566No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA4520.27733804562578995No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCACTCACT4330.2656800304335555No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT4240.26015781271091803No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC4010.24604547853084466No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA3860.23684178232644898No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGTGTAAAACGACG3850.23622820257948926No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA3820.2343874633386101No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCTCTTTCTCTAG3760.23070598485685187No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGAGCTGAGCCCTC3590.22027512915853673No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC3530.21659365067677847No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA3410.20923069371326192No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAGAAATCAGGC3340.2049356354845439No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGCCATCTCTGCTC3110.19082330130447053No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGAAGCCTCAGGACGTA3080.1889825620635914No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG3010.18468750383487342No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG2870.17609738737743744No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCATAGCTGTT2860.17548380763047772No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCAGGAGGATCCTC2810.17241590889567918No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGGGCTGAAAAGAT2770.1699615899078403No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGTGTAAAACGACG2690.16505295193216263No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT2680.1644393721852029No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCCGAGGATCCTCC2620.16075789370344462No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGCTTGCAGCACA2510.1540085164868878No Hit
TGTAAAACGACGGCCAGTAGGTGGCAAAGCTCAATCAGGACAGTTCTGAA2490.15278135699296838No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGAGACAGGTCTA2490.15278135699296838No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC2470.15155419749904894No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACGGCACTGG2460.15094061775208922No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCAGGATCCTCCCT2450.15032703800512953No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCGAGGGAGGATCC2450.15032703800512953No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT2430.14909987851121012No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCTGCAGTGGAGGAT2420.14848629876425037No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCTTGTGACAATGC2410.14787271901729068No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCGCAGGCTGGA2410.14787271901729068No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGTGAGGCAGGGAC2370.1454184000294518No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGAAATCAGGCT2340.1435776607885727No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG2280.13989618230681442No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCTGGGCTCAGATG2240.13744186331897557No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGGCCCTTCCAGGA2160.13253322534329787No Hit
TGTAAAACGACGGCCAGTGGGGACAAGGGTGGCTTTGTAAATAGCAGCAA2120.13007890635545902No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT2060.12639742787370073No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCTAGGCTCAAGAG2000.12271594939194247No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGAGCCAACAATGGCCT1980.12148878989802306No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCTGGGAGAGT1890.11596657217538564No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC1850.11351225318754679No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT1830.11228509369362737No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCTGTGGTGGGGGAC1670.10246781774227195No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCGCTCACT1650.10124065824835254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)