FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125440
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC26562.11734693877551No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG22611.802455357142857No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC20601.642219387755102No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT17851.4229910714285714No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT17031.3576211734693877No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA16211.292251275510204No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA16111.2842793367346939No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG15011.1965880102040816No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT13671.089764030612245No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG12801.0204081632653061No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC12460.9933035714285714No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG11400.9088010204081632No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC9480.7557397959183674No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG8510.6784119897959183No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT7820.623405612244898No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT7800.6218112244897959No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG7030.5604272959183674No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA6910.5508609693877551No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA6830.5444834183673469No Hit
CAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT6820.543686224489796No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA6790.5412946428571428No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC6460.5149872448979592No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG6190.4934630102040817No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT6060.4830994897959183No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA5760.4591836734693878No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT5570.4440369897959184No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4870.3882334183673469No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG4770.38026147959183676No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG4530.36112882653061223No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA4450.3547512755102041No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC4250.3388073979591837No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4210.3356186224489796No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA3970.3164859693877551No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT3800.30293367346938777No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3760.2997448979591837No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3520.28061224489795916No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG3480.2774234693877551No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3460.2758290816326531No Hit
CAGGAAANNGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT3310.26387117346938777No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT3210.25589923469387754No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA3170.25271045918367346No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3030.24154974489795916No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG3020.24075255102040816No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2950.23517219387755103No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCAGAGAGTGATAT2880.2295918367346939No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2880.2295918367346939No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG2770.22082270408163265No Hit
CAGGAAANAGCTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC2760.22002551020408162No Hit
CAGGAAANAGCTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC2760.22002551020408162No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA2740.2184311224489796No Hit
CAGGAAANNGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG2690.2144451530612245No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2610.2080676020408163No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2550.20328443877551022No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2540.2024872448979592No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC2510.20009566326530615No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2510.20009566326530615No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2480.19770408163265304No Hit
CAGGAAACNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2450.1953125No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2400.1913265306122449No Hit
CAGGAAANAGCTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC2390.19052933673469388No Hit
CAGGAAANNGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2360.1881377551020408No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2360.1881377551020408No Hit
CAGGAAACAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2260.18016581632653061No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2150.17139668367346939No Hit
CAGGAAACAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2100.16741071428571427No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG2100.16741071428571427No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2080.16581632653061223No Hit
CAGGAAACNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2030.16183035714285715No Hit
CAGGAAACNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2020.16103316326530612No Hit
CAGGAANNNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2010.16023596938775508No Hit
CAGGAAANNGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG2000.15943877551020408No Hit
CAGGAAANNGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1990.15864158163265304No Hit
NAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1930.15385841836734693No Hit
CAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1900.15146683673469388No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1890.15066964285714288No Hit
CAGGAAANNGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA1830.14588647959183673No Hit
CAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1820.14508928571428573No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1810.1442920918367347No Hit
CAGGAAANAGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT1810.1442920918367347No Hit
CAGGAAACAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1780.14190051020408165No Hit
CAGGAANNNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1770.14110331632653061No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1750.13950892857142858No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1750.13950892857142858No Hit
NAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1750.13950892857142858No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC1700.13552295918367346No Hit
NAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1690.13472576530612246No Hit
CAGGAANNNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1690.13472576530612246No Hit
NAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1670.1331313775510204No Hit
CAGGAAANNGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1640.13073979591836735No Hit
CAGGAAACAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1590.12675382653061223No Hit
NAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1570.1251594387755102No Hit
NAGGANANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1570.1251594387755102No Hit
CAGGAAACAGCTATGANCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1520.1211734693877551No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1500.11957908163265306No Hit
CAGGAAACAGCTATGANCCAGGGCTCCAAGAATGTTCCACACATCTTTCT1500.11957908163265306No Hit
CAGGAAANNGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC1460.116390306122449No Hit
CAGGAAACNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1460.116390306122449No Hit
CAGGAAACAGCTATGANCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1450.11559311224489796No Hit
CAGGAAACNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1450.11559311224489796No Hit
CAGGAAACNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1440.11479591836734696No Hit
NAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1410.11240433673469388No Hit
NAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1400.11160714285714285No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1370.1092155612244898No Hit
NAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1370.1092155612244898No Hit
CAGGAANNNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1360.10841836734693877No Hit
NAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1360.10841836734693877No Hit
CAGGAAACNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1360.10841836734693877No Hit
CAGGAANNNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1350.10762117346938774No Hit
CAGGAAANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1340.10682397959183673No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTGTAATCCCAGCT1330.10602678571428571No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1330.10602678571428571No Hit
CAGGAAACAGCTATGANCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1330.10602678571428571No Hit
CAGGAAACNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1320.10522959183673469No Hit
NAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1320.10522959183673469No Hit
NAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1310.10443239795918367No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1300.10363520408163265No Hit
CAGGAAANNGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG1300.10363520408163265No Hit
CAGGAAANNGCTATGANNAATGCAAGGGACCACCAGGCAGAGAGTGATAT1290.10283801020408163No Hit
NAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1270.1012436224489796No Hit
CAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1270.1012436224489796No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)