FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125203
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA28222.253939602086212No Hit
TGTAAAANGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC25782.0590560929051223No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA24871.9863741284154535No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC24381.9472376859979392No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT15631.2483726428280473No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG15481.236392099230849No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG14991.1972556568133352No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC14031.120580177791267No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC13911.1109957429135084No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT13881.108599634194069No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG13061.043105995862719No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC12831.0247358290136817No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC12811.0231384232007221No Hit
TGTAAAANGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC12531.0007747418192854No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG12390.9895929011285671No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA11800.9424694296462545No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC11670.932086291862016No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA11260.8993394726963412No Hit
TGTAAAANGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG10350.8266575082066723No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA10120.8082873413576352No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA9810.783527551256759No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT9740.7779366309113999No Hit
TGTAAAANGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT9120.7284170507096476No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA9000.7188326158318891No Hit
TGTAAAANGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT9000.7188326158318891No Hit
TGTAAAANGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC8870.7084494780476507No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG8860.7076507751411707No Hit
TGTAAAANGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT8730.6972676373569323No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG8670.6924754199180531No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC8520.6804948763208549No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT8480.6773000646949354No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT8200.6549363833134989No Hit
TGTAAAANGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG7910.6317739990255824No Hit
TGTAAAANGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT7900.6309752961191026No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC7750.6189947525219044No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT7590.6062155060182264No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG7530.6014232885793471No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA7350.5870466362627094No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT7320.5846505275432697No Hit
TGTAAAANGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC7070.5646829548812727No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC7020.5606894403488734No Hit
TGTAAAANGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT6950.5550985200035142No Hit
TGTAAAANGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG6840.5463127880322357No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG6680.5335335415285576No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC6640.5303387299026382No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT6580.5255465124637588No Hit
TGTAAAACGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG6510.5199555921183997No Hit
TGTAAAANGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA6370.5087737514276814No Hit
TGTAAAANGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT6210.49599450492400343No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT6050.48321525842032537No Hit
TGTAAAANGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT6000.479221743887926No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG5820.4648450915712882No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG5820.4648450915712882No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG5810.4640463886648083No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC5800.4632476857583285No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG5760.4600528741324089No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5740.4584554683194493No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA5690.4544619537870498No Hit
TGTAAAANGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT5620.4488710334416907No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT5620.4488710334416907No Hit
TGTAAAANGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA5610.4480723305352108No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG5540.44248141018985165No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT5340.42650735206025414No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG5330.4257086491537743No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG5250.4193190259019352No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT5240.4185203229954554No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC5230.41772162008897556No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC5220.41692291718249563No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG5200.4153255113695358No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG5180.4137281055565761No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5150.41133199683713645No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG5150.41133199683713645No Hit
TGTAAAANGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC5150.41133199683713645No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG5110.408137185211217No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT5110.408137185211217No Hit
TGTAAAANGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT5050.4033449677723377No Hit
TGTAAAANGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG5010.4001501561464182No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG4950.39535793870753894No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG4880.3897670183621798No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA4870.38896831545569993No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT4860.3881696125492201No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC4820.38497480092330055No Hit
TGTAAAANGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC4680.3737929602325823No Hit
TGTAAAANGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG4560.3642085253548238No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC4500.35941630791594453No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4490.3586176050094646No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC4480.3578189021029847No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC4470.35702019919650485No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG4420.3530266846641055No Hit
TGTAAAANGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG4390.35063057594466585No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC4330.3458383585057866No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG4300.34344224978634696No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG4250.3394487352539476No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT4210.3362539236280281No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT4170.3330591120021086No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG4120.3290655974697092No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG4040.3226759742178702No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG4010.32027986549843057No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA3980.31788375677899094No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG3870.30909802480771226No Hit
TGTAAAACGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG3640.2907278579586751No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3540.28274082889387636No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC3490.278747314361477No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT3480.2779486114549971No Hit
TGTAAAANGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT3310.26437066204483917No Hit
TGTAAAANGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC3220.2571823358865203No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC3200.25558493007356053No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3140.2507927126346813No Hit
TGTAAAANGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC2880.23002643706620446No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC2860.22842903125324474No Hit
TGTAAAACGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT2840.22683162544028498No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC2670.21325367603012707No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT2560.20446794405884844No Hit
TGTAAAANGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT2500.19967572661996916No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT2340.18689648011629115No Hit
TGTAAAANGACGGCCAGTGACCTCATGGGGCGCTTCCGCGTGCTGGGCTG2150.17172112489317348No Hit
TGTAAAANGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA2100.1677276103607741No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT2010.1605392842024552No Hit
TGTAAAANGACGGCCAGTTGATGACCTCTTGGCTAACATACACAACATGA1780.14216911735341803No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1730.13817560282101865No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA1690.13498079119509918No Hit
TGTAAAANGACGGCCAGTCTTGCACTACATGGCCATCATGAACCAGCGAA1680.13418208828861927No Hit
TGTAAAACGACGGCCAGTTGATGACCTCTTGGCTAACATACACAACATGA1670.1333833853821394No Hit
TGTAAAACGACGGCCAGTGACCTCATGGGGCGCTTCCGCGTGCTGGGCTG1520.12140284178494126No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCATGAACCAGCGAA1470.11740932725254187No Hit
TGTAAAANGACGGCCAGTGGCTCCCCAGCTGTCTCTCTCCTCTCTGGGCT1380.11022100109422298No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1330.1062274865618236No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGGAAGACTGGCAGGT1300.10383137784238396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)