FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125203
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT10400.8306510227390717No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC10290.8218652907677931No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT10100.8066899355446754No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT9870.7883197686956382No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC9710.7755405221919602No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT9530.7611638698753225No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA9220.7364040797744463No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA9090.726020941990208No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG8530.6812935792273348No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA8150.6509428687810995No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT7960.6357675135579819No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA7540.602221991485827No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT7430.5934362595145484No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT6800.5431179764063161No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA6630.5295400269961582No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG6620.5287413240896783No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC6480.5175594833989601No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG6220.49679320783048325No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA6160.49200099039160405No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT6130.4896048816721644No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT6030.48161785260736567No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT6020.48081914970088574No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC5900.47123471482312723No Hit
CAGGAAANAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT5860.4680399031972077No Hit
CAGGAAANAGCTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA5800.4632476857583285No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT5630.4496697363481706No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT5210.41612421427601576No Hit
CAGGAAANAGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG5200.4153255113695358No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA5190.414526808463056No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG5170.41292940265009626No Hit
CAGGAAANAGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC5160.4121306997436164No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA5030.40174756195937794No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC4740.37858517767146155No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT4670.3729942573261024No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC4470.35702019919650485No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4330.3458383585057866No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4260.34024743816042746No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC4150.33146170618914883No Hit
CAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT4040.3226759742178702No Hit
CAGGAAANAGCTATGANCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4030.3218772713113903No Hit
CAGGAAANAGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT3990.3186824596854708No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC3960.31628635096603114No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT3940.31468894515307144No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG3940.31468894515307144No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3810.304305807368833No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3740.2987148870234739No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT3730.297916184116994No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3720.2971174812105141No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3680.2939226695845946No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3680.2939226695845946No Hit
CAGGAAANAGCTATGANCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT3660.29232526377163487No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT3590.28673434342627574No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3570.285136937613316No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAATT3540.28274082889387636No Hit
CAGGAAANAGCTATGANCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3520.2811434230809166No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAACT3520.2811434230809166No Hit
CAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3490.278747314361477No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG3480.2779486114549971No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC3470.27714990854851723No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3450.2755525027355574No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA3440.2747537998290776No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT3430.27395509692259773No Hit
CAGGAAANAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3400.27155898820315805No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT3380.26996158239019835No Hit
CAGGAAANAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG3380.26996158239019835No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC3370.2691628794837185No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA3330.2659680678577989No Hit
CAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3320.2651693649513191No Hit
CAGGAAANAGCTATGANCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3300.2635719591383593No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT3280.26197455332539954No Hit
CAGGAAANAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA3180.2539875242606008No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC3160.25239011844764103No Hit
CAGGAAANAGCTATGANNGGGTCCCACCCATGCTGAAAGTCCACCTTCCA3110.24839660391524163No Hit
CAGGAAANAGCTATGANCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3010.24040957485044287No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2980.23801346613100327No Hit
CAGGAAANAGCTATGANNAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT2960.2364160603180435No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2950.23561735741156362No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2930.23401995159860384No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA2910.2324225457856441No Hit
CAGGAAANAGCTATGACCACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC2900.23162384287916424No Hit
CAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG2880.23002643706620446No Hit
CAGGAAANAGCTATGANCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2880.23002643706620446No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2830.22603292253380508No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2820.2252342196273252No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT2750.2196432992819661No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA2750.2196432992819661No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2750.2196432992819661No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2650.2116562702171673No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC2650.2116562702171673No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2620.2092601614977277No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2600.2076627556847679No Hit
CAGGAAANAGCTATGANCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2530.2020718353394088No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2520.20127313243292894No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2500.19967572661996916No Hit
CAGGAAANAGCTATGANNTCCACAAGGCCCTGGGACAATCTCCCAGGCCC2490.19887702371348928No Hit
CAGGAAANAGCTATGANNCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT2470.19727961790052956No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG2450.19568221208756978No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2430.19408480627461006No Hit
CAGGAAANAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT2390.19088999464869053No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2390.19088999464869053No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2360.1884938859292509No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2320.18529907430333137No Hit
CAGGAAANAGCTATGANCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2250.17970815395797227No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGTGACACA2250.17970815395797227No Hit
CAGGAAANAGCTATGANCACAAACGCAAATGGCTTTTAGCATTTCACAGG2230.17811074814501252No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC2190.17491593651909298No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2190.17491593651909298No Hit
CAGGAAANAGCTATGANCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA2160.17251982779965336No Hit
CAGGAAANAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC2140.1709224219866936No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG2140.1709224219866936No Hit
CAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC2120.16932501617373386No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA2100.1677276103607741No Hit
CAGGAAANAGCTATGANCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2060.1645327987348546No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2000.15974058129597535No Hit
CAGGAAACAGCTATGACCACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC1930.1541496609506162No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1930.1541496609506162No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1910.15255225513765644No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1910.15255225513765644No Hit
CAGGAAANAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA1890.15095484932469672No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1890.15095484932469672No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1890.15095484932469672No Hit
CAGGAAANAGCTATGANNGGTCCCGCGGGGAAAGTAAGTCCTTCCCTTGG1870.14935744351173694No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1870.14935744351173694No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1870.14935744351173694No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT1870.14935744351173694No Hit
CAGGAAANAGCTATGANCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1840.1469613347922973No Hit
CAGGAAANAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT1830.14616263188581743No Hit
CAGGAAANAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC1830.14616263188581743No Hit
CAGGAAANAGCTATGANCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1830.14616263188581743No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1760.1405717115404583No Hit
CAGGAAANAGCTATGANNTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT1750.1397730086339784No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1750.1397730086339784No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1730.13817560282101865No Hit
CAGGAAANAGCTATGANCACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC1730.13817560282101865No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1710.13657819700805893No Hit
CAGGAAANAGCTATGANCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1700.13577949410157902No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1690.13498079119509918No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1670.1333833853821394No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1610.12859116794326014No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1600.12779246503678027No Hit
CAGGAAANAGCTATGANNGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG1590.1269937621303004No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1590.1269937621303004No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1590.1269937621303004No Hit
CAGGAAACAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT1550.12379895050438089No Hit
CAGGAAANAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA1540.12300024759790101No Hit
CAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA1540.12300024759790101No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC1520.12140284178494126No Hit
CAGGAAANAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT1520.12140284178494126No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1510.12060413887846139No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1500.1198054359719815No Hit
CAGGAAANAGCTATGACCTCCACAAGGCCCTGGGACAATCTCCCAGGCCC1490.11900673306550164No Hit
CAGGAAANAGCTATGANCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1460.11661062434606201No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1420.11341581272014249No Hit
CAGGAAANAGCTATGANNAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT1420.11341581272014249No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1400.11181840690718274No Hit
CAGGAAANAGCTATGANCAAACTATTAATACCTGCAGCAAAGCCCCAAGC1390.11101970400070285No Hit
CAGGAAANAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT1380.11022100109422298No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1350.10782489237478336No Hit
CAGGAAANAGCTATGANCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1330.1062274865618236No Hit
CAGGAAANAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1310.10463008074886385No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1300.10383137784238396No Hit
CAGGAAANAGCTATGANCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA1260.10063656621646447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)