FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117210
Sequences flagged as poor quality0
Sequence length300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC42373.614879276512243No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA41543.544066205955123No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC33552.862383755652248No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG27182.318914768364474No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT20751.7703267639279925No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT19651.6764781162016893No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG18261.5578875522566336No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG17031.4529477007081306No Hit
TGTAAAACGACGGCCAGTTCCTCCTTTGCCAATATAGGGTTTGATGTAAT16681.4230867673406706No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC16301.3906663253988567No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG15361.3104683900691068No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG14241.2149134032932343No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC14101.2029690299462503No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT13991.19358416517362No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG13991.19358416517362No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC13541.155191536558314No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG13431.1458066717856839No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG13421.1449535022608992No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT13121.119358416517362No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC12741.086937974575548No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG12701.08352529647641No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT12331.0519580240593807No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC11490.9802917839774763No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC11200.9555498677587237No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA10900.9299547820151864No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT10490.8949748314990189No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA10400.8872963057759576No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGTT9910.8454909990615135No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC9900.8446378295367288No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG9850.8403719819128062No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT9630.8216022523675454No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT9490.8096578790205614No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT9120.7780906066035321No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC8990.7669994027813326No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC8130.6936268236498592No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC8100.6910673150755056No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA7990.6816824503028751No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG7900.6740039245798141No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC7550.644142991212354No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTTGGCCTCC7090.6048971930722634No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT6570.5605323777834655No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCGACCACACCACA6460.5511475130108352No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG6370.543468987287774No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT6270.5349372920399283No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT6240.5323777834655746No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC6200.5289651053664363No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC6200.5289651053664363No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT6130.5229929186929443No Hit
TGTAAAACGACGGCCAGTATCTGCACATCGTGCTGCCACACGAGGCTTCA6100.5204334101185906No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC5780.4931319853254842No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG5470.46668373005716235No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGTTTGGATGTGCC5370.4581520348093166No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT5300.4521798481358246No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA5110.43596962716491766No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC5050.43085061001621017No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC4920.41975940619401075No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGGTGGCTGCGC4740.40440235474788844No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC4300.3668628956573671No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA4300.3668628956573671No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT4230.3608907089838751No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA4230.3608907089838751No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG4220.36003753945909056No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG4050.3455336575377528No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA4000.3412678099138299No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT3890.33188294514119954No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG3830.3267639279924921No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG3780.32249808036856925No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGCACTCTTGGTG3750.31993857179421553No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA3720.31737906321986176No Hit
TGTAAAACGACGGCCAGTAGGTGGCAAAGCTCAATCAGGACAGTTCTGAA3610.30799419844723147No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGAGGTGG3590.30628785939766234No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG3520.3003156727241703No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCAGCACCCGGCAGC3460.2951966555754628No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCGCAGGCTGGA3350.28581179080283253No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA3240.2764269260302022No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTCAGGCGGGAGG3110.2653357222080027No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCCTAAGGGAG3040.2593635355345107No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGAACCTGGGA2980.25424451838580325No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA2890.2465659926627421No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGCTGTCATCTCT2850.2431533145636038No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGAACACAGGGCGG2810.23974063646446547No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2760.2354747888405426No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCCAAGAGGTGGG2750.23462161931575806No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGATGGTGTGGC2640.22523675454312772No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCCAGCGGCTTCA2640.22523675454312772No Hit
TGTAAAACGACGGCCAGTGGGCAGGGGAGTCAGCAGAGACCAGACGGGAG2600.2218240764439894No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC2530.2158518897704974No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGGAGACCCTCC2520.21499872024571282No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCCCCGGACACCA2510.21414555072092825No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGTGAGGACGTGG2450.20902653357222079No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC2410.2056138554730825No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG2400.2047606859482979No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC2390.20390751642351332No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCCCCC2380.20305434689872878No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAAGAGAGCATAGA2380.20305434689872878No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCGGTGCCCCGGCAG2290.1953758211756676No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA2290.1953758211756676No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGCTGTTTCCTGA2130.1817251087791144No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT2120.18087193925432982No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG2090.1783124306799761No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCCGGAGTGCAATG2060.1757529221056224No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCGTGGGGCACTCTTGGTG2000.17063390495691494No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCTTGTGACAATGA1990.16978073543213035No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCGCGCAGGGCAG1970.16807439638256122No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGAGAAGGGGC1960.16722122685777663No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGCGTCATGGCT1890.16124904018428463No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGGGTGGA1870.15954270113471547No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGGCAAGAGGTGCT1790.15271734493643888No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA1780.15186417541165428No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC1770.15101100588686972No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT1720.14674515826294685No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT1710.14589198873816228No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACAGCACTGCC1700.1450388192133777No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGTCTGGTAGTAG1640.13991980206467025No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG1630.1390666325398857No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTTGTAAAACGACG1620.1382134630151011No Hit
TGTAAAACGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG1580.1348007849159628No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCATGGACAGGCC1520.12968176776725535No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCCGGCAGCGACAT1380.1177373944202713No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACAGCAGCTCTGG1330.11347154679634842No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT1290.11005886869721014No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA1240.10579302107328727No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTTACTGCAACC1230.10493985154850269No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG1230.10493985154850269No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCATTATCTGGGA1180.10067400392457981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)