FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117210
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT40613.4647214401501576No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA30832.6303216449108437No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC27722.364985922702841No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT23962.044194181383841No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT22991.961436737479737No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT19871.69524784574695No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT19371.6525893695077212No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT18341.56471290845491No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC16721.4264994454398088No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG15091.2874328128999233No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC14451.2328299633137105No Hit
CAGGAAACAGCTATGACCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT13851.181639791826636No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG13761.1739612661035748No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC13331.137274976537838No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG13011.1099735517447318No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT12671.0809657879020562No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC12631.0775531098029179No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC12551.0707277536046413No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC12431.0604897193072262No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT10920.9316611210647555No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG10250.874498762904189No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC10250.874498762904189No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT9880.8429314904871598No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT9840.8395188123880215No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT9340.7968603361487927No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC9200.7849159628018086No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT9180.7832096237522396No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA8960.764439894206979No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA8800.7507891818104258No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA8620.7354321303643034No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA8270.7055711969968433No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC7830.668031737906322No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC7510.6407303131132156No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG7250.6185479054688167No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT7220.6159883968944629No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG6730.5741830901800188No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA6520.5562665301595427No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC6410.5468816653869123No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGTGTGGTCGGCA5830.49739783294940704No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA5620.47948127292893095No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG5400.4607115433836704No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTAGGGTCTGGGGC5130.43767596621448684No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA4970.4240252538179336No Hit
CAGGAAACAGCTATGACCACCCCACCTGCTCAAAAGACACACTCCTAAAA4960.42317208429314906No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGGACATCACAAGC4950.4223189147683644No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC4780.40781503284702664No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA4770.4069618633222421No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC4740.40440235474788844No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT4530.38648579472741235No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC4520.38563262520262775No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGGCGCAGCCACC4320.36856923470693626No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC4000.3412678099138299No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3860.3293234365668459No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG3860.3293234365668459No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA3830.3267639279924921No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA3820.3259107584677075No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA3740.31908540226943094No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT3640.3105537070215852No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCAAGACACTGGCAGGT3630.3097005374968006No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT3430.29263714700110915No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCCGTCCTCCCAGC3350.28581179080283253No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGTGCTGCCTCTCCA3310.2823991127036942No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACTCCTGTAA3280.2798396041293405No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA3240.2764269260302022No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3200.2730142479310639No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGATGTCGTCATCC3200.2730142479310639No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3190.27216107840627934No Hit
CAGGAAACAGCTATGACCACCCCTTGCATTGGTAGGGCTTCTCTCCTGTG3140.26789523078235644No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC3090.2636293831584336No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC3050.2602167050592953No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG3030.2585103660097261No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCGC3010.256804026960157No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCTCCCTTAGGAGT2990.25509768791058784No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACGCACGGC2790.23803429741489635No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACACACGGC2790.23803429741489635No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2750.23462161931575806No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2730.23291528026618888No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTCTAGCAGGCAC2700.2303557716918352No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGGACACTGCCCA2690.2295026021670506No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT2680.22864943264226603No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCCCCTCCCTCCC2640.22523675454312772No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCAGGAAACAGCT2590.22097090691920485No Hit
CAGGAAACAGCTATGACCACTCCCAACTCCCGTCTGGTCTCTGCTGACTC2560.2184113983448511No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2510.21414555072092825No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCATCCTGCCTCC2490.21243921167135912No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGGGGCCCTCTTCC2430.20732019452265166No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2400.2047606859482979No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2390.20390751642351332No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCCAGGAGGGTCT2390.20390751642351332No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGGCACCGCCTCTCC2380.20305434689872878No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT2310.19708216022523675No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGGTGGGATGG2310.19708216022523675No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGGGGC2300.19622899070045216No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2290.1953758211756676No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2270.19366948212609847No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTGATGCACCCCC2270.19366948212609847No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2250.19196314307652929No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACACGGCTCTATGC2150.18343144782868356No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCGCAGTGGCTGAT2110.18001876972954528No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC2100.1791656002047607No Hit
CAGGAAACAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC2060.1757529221056224No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC2040.17404658305605322No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACTGGCAGGT2010.1714870744816995No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCCGT1970.16807439638256122No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1970.16807439638256122No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1930.16466171828342294No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG1900.1621022097090692No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACCGCCCCCTGCCCT1800.15357051446122344No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCACTCAGCGTGTG1790.15271734493643888No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGATGCCAACCAAC1740.148451497312516No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG1680.14333248016380856No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTCCACCCTACTAC1670.14247931063902397No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGAGGCTGTAGGG1670.14247931063902397No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCATCGCTACACTC1640.13991980206467025No Hit
CAGGAAACAGCTATGACCCATGTGCCCCACTGATGCTCTCAATGTGTACT1630.1390666325398857No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGACCAGGGCCTG1620.1382134630151011No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1520.12968176776725535No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1520.12968176776725535No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT1410.12029690299462503No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCTCTCCACGTGC1400.11944373346984047No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1390.11859056394505588No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGAAACAGCTATG1380.1177373944202713No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG1240.10579302107328727No Hit
CAGGAAACAGCTATGACCACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC1230.10493985154850269No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTGGGCATCCTGTA1230.10493985154850269No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1180.10067400392457981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)