FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122940
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT24251.9725069139417604No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC22941.8659508703432568No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC18951.5414023100699528No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC18711.5218805921587766No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT16601.3502521555230194No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG15611.2697250691394177No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC13871.1281926142833902No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT13821.124125589718562No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT13371.0875223686351065No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT13251.0777615096795183No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT11790.95900439238653No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT10890.8857979502196193No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT9240.7515861395802831No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC9140.7434520904506263No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG8850.7198633479746218No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC7540.6133073043761184No Hit
CAGGAAANAGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC7420.6035464454205304No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG7420.6035464454205304No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG7410.6027330405075647No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC7340.5970392061168049No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA6970.566943224337075No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT6580.5352204327314137No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG6100.49617699690906136No Hit
CAGGAAANAGCTATGANNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT6070.4937367821701643No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6000.4880429477794046No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT5970.48560273304050755No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG5880.47828208882381645No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT5460.4441190824792582No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG5430.44167886774036114No Hit
CAGGAAANNGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC5350.4351716284366358No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG5330.43354481861070443No Hit
CAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT4840.39368797787538634No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4110.3343094192288921No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3890.3164145111436473No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG3880.3156011062306816No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3800.30909386692695623No Hit
CAGGAAANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT3720.30258662762323085No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT3640.2960793883195055No Hit
CAGGAAANAGCTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC3470.28225150479908895No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT3350.27249064584350086No Hit
CAGGAAANNGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG3260.2651700016268098No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG3130.25459573775825606No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG3030.24646168862859932No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2980.24239466406377091No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2930.2383276394989426No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2910.2367008296730112No Hit
CAGGAAANAGCTATGANNACCCCACCTGCTCAAAAGACACACTCCTAAAA2900.23588742476004557No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2700.21961932650073207No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2630.21392549210997236No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA2620.21311208719700667No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2600.21148527737107534No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG2550.20741825280624696No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2520.20497803806734993No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2510.20416463315438427No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2480.20172441841548724No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2450.19928420367659022No Hit
CAGGAAANNGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC2450.19928420367659022No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC2290.1862697250691394No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2210.17976248576541404No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2200.17894908085244834No Hit
CAGGAAANNGCTATGANNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT2180.17732227102651701No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2100.1708150317227916No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC2080.16918822189686025No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCAG2080.16918822189686025No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2070.1683748169838946No Hit
NAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2030.1651211973320319No Hit
CAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2010.16349438750610054No Hit
CAGGAAANNGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG2000.16268098259313485No Hit
NAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1990.16186757768016918No Hit
CAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1990.16186757768016918No Hit
NAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1970.16024076785423785No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG1950.1586139580283065No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1890.15373352855051245No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1860.15129331381161543No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1830.1488530990727184No Hit
CAGGAAANAGCTATGANNACCTCTCTGGGCCTGTCTCCTGCCGTGTGCGC1830.1488530990727184No Hit
NAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1820.14803969415975274No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1790.1455994794208557No Hit
CAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1780.14478607450789002No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1770.14397266959492436No Hit
NAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1750.142345859768993No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1740.14153245485602733No Hit
NAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1720.13990564503009598No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1720.13990564503009598No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG1700.13827883520416462No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1670.13583862046526762No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1620.13177159590043924No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT1580.12851797624857655No Hit
CAGGAAANAGCTATGANNGGTCCCGCGGGGAAAGTAAGTCCTTCCCTTGG1570.12770457133561086No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTATTCCCAACCA1570.12770457133561086No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1570.12770457133561086No Hit
NAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1550.1260777615096795No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1540.12526435659671384No Hit
NAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1540.12526435659671384No Hit
NAGGAAANNGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1530.12445095168374816No Hit
CAGGANANNGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1520.1236375467707825No Hit
CAGGAAANAGCTATGANNCGGGACCCAAAGTTCTGCGTGATGGTCAGCAC1490.12119733203188546No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1490.12119733203188546No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1480.1203839271189198No Hit
NAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1460.11875711729298845No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1460.11875711729298845No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1450.11794371238002278No Hit
CAGGAAANNGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1450.11794371238002278No Hit
CAGGAANNNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1430.11631690255409144No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1430.11631690255409144No Hit
CAGGAAANNGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG1420.11550349764112575No Hit
CAGGAAANNGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1420.11550349764112575No Hit
CAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1410.11469009272816008No Hit
CAGGAAACAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1410.11469009272816008No Hit
CAGGAAANNGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1370.11143647307629738No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1360.1106230681633317No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG1340.10899625833740034No Hit
CAGGANANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1340.10899625833740034No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA1330.10818285342443469No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG1320.107369448511469No Hit
NAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1320.107369448511469No Hit
CAGGAAANAGCTATGANNTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC1300.10574263868553767No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1290.10492923377257199No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT1290.10492923377257199No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCTGTGA1280.10411582885960632No Hit
CAGGAAACNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1280.10411582885960632No Hit
CAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1260.10248901903367497No Hit
NAGGANANNGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1260.10248901903367497No Hit
NAGGANANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1260.10248901903367497No Hit
NAGGANANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1250.10167561412070929No Hit
CAGGAAANNGCTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC1240.10086220920774362No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1240.10086220920774362No Hit
CAGGANANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1230.10004880429477793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)