FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169669
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA79844.705632731966358No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG58593.4531941603946508No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT55903.2946501718051033No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG46262.7264850974544554No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA45732.695247806022314No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG41732.4594946631382277No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG39872.3498694516971277No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC33771.9903459087988966No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT33471.9726644230825903No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA31011.8276762402088773No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT30031.769916720202276No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT29681.7492883201999185No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG27691.6320011316150858No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG26501.5618645716070703No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTGTAAAACGACG23471.3832815658723752No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG19751.164031142990175No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC19711.161673611561334No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC19571.1534222515603911No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC17951.0579422286923363No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA13270.7821110515179556No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT13250.7809322858035351No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC13170.7762172229458534No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT13110.7726809258025922No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC10000.5893828572102151No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTGTAAAACGACG9670.5699332229222781No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA8700.5127630857728872No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT7730.4555929486234963No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG7450.43909022862161035No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA7280.4290707200490367No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC6840.4031378743317872No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG6130.3612916914698619No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTCACAGCCATGCA6100.3595235428982313No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC5460.32180304003677745No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG5340.3147304457502549No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC5010.2952808114623178No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4820.2840825371753237No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC4780.2817250057464829No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA4780.2817250057464829No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4750.27995685717485225No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTTGTAAAACGACG4190.24695141717108018No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA4110.24223635431339843No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA4080.24046820574176778No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG4040.23811067431292696No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT4030.2375212914557167No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC3990.23516376002687583No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG3770.2221973371682511No Hit
TGTAAAACGACGGCCAGTCACCAGCCATCTGGACCTGGTCATAGCTGTTT3740.22042918859662047No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCATAGCTGTTTCC3430.2021583200231038No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCAGGTGTA3160.186244982878428No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC3160.186244982878428No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTAAAACGACGG3130.18447683430679734No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTGACC2890.1703316457337522No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT2880.16974226287654198No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTCCTGCTCA2860.16856349716212154No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC2820.1662059657332807No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGATTTGGTGCTCTG2710.15972275430396832No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGGCGCCACCACAC2460.14498818287371293No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2230.13143237715787798No Hit
TGTAAAACGACGGCTAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA2190.12907484572903713No Hit
TGTAAAACGACGGCCAGTTAACCACAGTGGCCAGGGCGGTCATAGCTGTT2150.12671731430019625No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGCTCTGGTG2090.12318101715693497No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG2080.12259163429972476No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAACACCTCCACGCAGGA2030.11964472001367368No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTAACC1960.11551904001320219No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG1920.11316150858436132No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT1920.11316150858436132No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG1890.11139336001273067No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCTCAACAAGGAGACGATG1880.11080397715552046No Hit
TGNAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAATCTCCACGCAGGA1860.10962521144110002No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA1850.10903582858388981No Hit
TGNAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAATAAGGAGACGATG1800.10608891429783873No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)