FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169669
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT42192.486606274569898No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG40622.3940731659878938No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA29761.7540033830576003No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT28561.6832774401923747No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC26071.536521108747031No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC24761.4593119544524928No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT22381.3190388344364616No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT19271.1357407658440848No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC17471.0296518515462458No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC17401.0255261715457744No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC17071.0060765372578373No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC16510.9730710972540653No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG15170.8940937943878964No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT15120.8911468801018454No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG14050.8280829143803523No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC13890.8186527886649889No Hit
CAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA11370.6701283086480146No Hit
CAGGAAANAGCTATGANNAGGTCCAGATGGCTGGTGACTGGCCGTCGTTT11080.6530362057889184No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT10680.6294608915005098No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC10150.5982236000683684No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA9620.566986308636227No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8800.5186569143449894No Hit
CAGGAAANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT8070.4756319657686437No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC7110.41905121147646296No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG6920.40785293718946886No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC6610.38958206861595224No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6290.37072181718522534No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT6290.37072181718522534No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG6260.3689536686135947No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG5790.3412526743247146No Hit
CAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC5740.3383057600386635No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5680.3347694628954022No Hit
CAGGAAANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC5560.32769686860887964No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG5510.32474995432282855No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT5010.2952808114623178No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4960.29233389717626673No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC4870.2870294514613748No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT4850.2858506857469544No Hit
CAGGAAANNGCTATGANNAGGTCCAGATGGCTGGTGACTGGCCGTCGTTT4830.28467192003253394No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT4620.2722948800311194No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG4390.2587390743152845No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4380.25814969145807426No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG4300.2534346286003925No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG4200.24754080002829035No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG4020.2369319085985065No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3930.23162746288361455No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC3850.22691240002593285No Hit
CAGGAAANAGCTATGANNCAAGCGGTGCATGGCTGTGGCCGTCGTTTTAC3710.21866104002498982No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC3570.21040968002404684No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT3390.19980078859426295No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG3350.19744325716542208No Hit
CAGGAAANAGCTATGANNCAAGCGGTGCATGGCTGTGACTGGTCCCTTAG3320.19567510859379145No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC3310.19508572573658123No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3260.19213881145053016No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG3240.19096004573610972No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3170.18683436573563822No Hit
CAGGAAANNGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3110.18329806859237693No Hit
NAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3020.177993622877485No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3000.17681485716306455No Hit
CAGGAAANNGCTATGANNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG3000.17681485716306455No Hit
NAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2990.17622547430585436No Hit
CAGGAAANAGCTATGANNGCATCACTGAGGGAGGATCCTCCCCGGGCAGA2960.17445732573422368No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2780.16384843430443982No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2780.16384843430443982No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2750.16208028573280917No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2740.16149090287559897No Hit
CAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2660.15677584001791725No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2650.15618645716070703No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2630.1550076914462866No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2620.15441830858907638No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2590.15265016001744575No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2570.1514713943030253No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2560.1508820114458151No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2510.14793509715976402No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2430.1432200343020823No Hit
CAGGAAACAGCNATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2420.14263065144487208No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2390.14086250287324145No Hit
CAGGAAANNGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2340.13791558858719036No Hit
CAGGANANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2340.13791558858719036No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2320.13673682287276992No Hit
NAGGAAANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2320.13673682287276992No Hit
CAGGAAACNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2300.13555805715834948No Hit
CAGGAAANNGCTATGANNGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT2180.1284854628718269No Hit
CAGGAAANNGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2170.1278960800146167No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC2130.12553854858577582No Hit
NAGGANANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2110.1243597828713554No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA2050.12082348572809412No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2050.12082348572809412No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2040.12023410287088389No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2000.11787657144204304No Hit
CAGGAAANNGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1990.11728718858483284No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCCAGGAAACAGCT1980.1166978057276226No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1970.1161084228704124No Hit
NAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG1960.11551904001320219No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAGTGTTCC1910.11257212572715111No Hit
NAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1910.11257212572715111No Hit
CAGGAANNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1910.11257212572715111No Hit
CAGGAAANNGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1900.11198274286994088No Hit
CAGGAAACNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1860.10962521144110002No Hit
CAGGAAACAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1850.10903582858388981No Hit
CAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1840.10844644572667958No Hit
NAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1800.10608891429783873No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT1790.10549953144062853No Hit
NAGGANANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1780.1049101485834183No Hit
NAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1750.10314200001178765No Hit
CAGGANANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1750.10314200001178765No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1740.10255261715457745No Hit
NAGGAAANAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1740.10255261715457745No Hit
NAGGANANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1730.10196323429736721No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)