FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84213
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4930.5854203032785912No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3690.4381746286202842No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3530.4191751867288898No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3490.4144253262560412No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3470.4120503960196169No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3230.38355123318252526No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT3130.3716765820004037No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3050.36217686105470653No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2990.3550520703454336No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2890.34317741916331207No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2790.33130276798119057No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2730.3241779772719176No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT2690.319428116799069No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2560.30399107026231104No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA2530.3004286749076746No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2460.2921164190801895No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT2390.28380416325270447No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2390.28380416325270447No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2370.28142923301628014No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2200.2612423260066736No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2200.2612423260066736No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2130.2529300701791885No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT2110.2505551399427642No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2070.24580527946991554No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2060.2446178143517034No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2000.2374930236424305No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1990.23630555852421833No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1990.23630555852421833No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1990.23630555852421833No Hit
NNGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT1980.2351180934060062No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1890.22443090734209684No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1830.2173061166328239No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1800.21374372127818744No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1790.21255625615997528No Hit
NNGGANNNNGNTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1680.19949413985964165No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1660.19711920962321733No Hit
NNGGANNNNGNTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT1650.1959317445050052No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1630.19355681426858087No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1620.1923693491503687No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1610.19118188403215655No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1590.18880695379573226No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1560.18524455844109577No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1550.18405709332288364No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1530.18168216308645935No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1520.1804946979682472No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1500.1781197677318229No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1480.17574483749539857No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1470.1745573723771864No Hit
NNGGANNNNGNTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1470.1745573723771864No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1460.17336990725897425No Hit
NAGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1440.17099497702254995No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1420.16862004678612566No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1410.1674325816679135No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCCAAACCAG1410.1674325816679135No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1370.1626827211950649No Hit
NNGGANNNNGNTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1360.16149525607685272No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1350.1603077909586406No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1280.15199553513115552No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1250.14843313977651906No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1230.14605820954009474No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1220.1448707444218826No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1210.14368327930367045No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1200.1424958141854583No Hit
NNGGANNNNGNTATGNNNACAAACGCAAATGGCTTTTAGCATTTCACAGG1190.14130834906724615No Hit
NNGGANNNNGNTATGNNNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1170.13893341883082186No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1160.1377459537126097No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1140.13537102347618538No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1130.13418355835797321No Hit
NAGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1120.13299609323976108No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1120.13299609323976108No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1120.13299609323976108No Hit
NNGGANNNNGNTATGNNNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT1110.13180862812154892No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1100.1306211630033368No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1100.1306211630033368No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1100.1306211630033368No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1100.1306211630033368No Hit
NNGGANNNNGNTATGNNNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1100.1306211630033368No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1080.12824623276691247No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1040.12349637229406388No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1040.12349637229406388No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1030.1223089071758517No Hit
NNGGANNNNGNTATGNNNGCAAAACACCTTAACACTCGGCTTCTATTTGC1020.12112144205763956No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1020.12112144205763956No Hit
NNGGANNNNGNTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1000.11874651182121525No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1000.11874651182121525No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT990.1175590467030031No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA990.1175590467030031No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC990.1175590467030031No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC980.11637158158479095No Hit
NNGGANNNNGNTATGNNNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT980.11637158158479095No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT970.11518411646657879No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT970.11518411646657879No Hit
NAGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA960.11399665134836665No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA960.11399665134836665No Hit
NNGGAAANNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT950.1128091862301545No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG950.1128091862301545No Hit
NNGGANNNNGNTATGNNNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT950.1128091862301545No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA940.11162172111194235No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC940.11162172111194235No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT940.11162172111194235No Hit
NAGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG910.10805932575730587No Hit
NNGGANNNNGNTATGNNNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT910.10805932575730587No Hit
NAGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT900.10687186063909372No Hit
NAGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT900.10687186063909372No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT900.10687186063909372No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT900.10687186063909372No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG880.10449693040266941No Hit
NAGGAAANAGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT880.10449693040266941No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG880.10449693040266941No Hit
NAGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT870.10330946528445727No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC870.10330946528445727No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG870.10330946528445727No Hit
NNGGANNNNGNTATGNNNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC850.10093453504803297No Hit
NNGGANNNNGNTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC850.10093453504803297No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)