FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92988
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT22902.4626833569923003No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC13941.4991181657848323No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT13831.4872886824106337No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA12701.3657676259302276No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT12691.364692218350755No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT12281.3206005075923777No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC11041.1872499677377726No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT8700.9356045941411795No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC8470.9108702198133093No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA8290.891512883382802No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG7030.7560115283692519No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6340.6818084053856411No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT6260.6732051447498602No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT5520.5936249838688863No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT5480.5893233535509957No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC5390.5796446853357422No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT5310.5710414246999613No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG5230.5624381640641803No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA5100.5484578655310363No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5070.5452316427926184No Hit
CAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA4980.5355529745773648No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT4920.5291005291005291No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT4900.5269497139415839No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4860.5226480836236934No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4140.4452187379016648No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA4080.43876629242482906No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT4000.430163031789048No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA3970.4269368090506302No Hit
CAGGAAANAGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT3730.40112702714328724No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3620.3892975437690885No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3560.3828450982922528No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3220.34628124059018367No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3150.33875338753387535No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3150.33875338753387535No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2920.31401901320600506No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG2800.30111412225233364No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2650.28498300856024433No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2640.2839076009807717No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2480.2667010797092098No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT2400.2580978190734288No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2380.25594700391448355No Hit
CAGGAAANAGCTATGANNATTAGCCAGGTGTGGTGGCGGGCGCATCCACC2340.2516453735965931No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2290.24626833569923No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2170.2333634447455586No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2130.22906181442766807No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2080.22368477653030497No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2070.2226093689508324No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1980.2129307007355788No Hit
CAGGAAANAGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATTCTTC1970.21185529315610616No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1960.21077988557663355No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1880.2021766249408526No Hit
CAGGAAANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1870.20110121736137995No Hit
CAGGAAANNGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1760.18927173398718114No Hit
CAGGAAANNGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT1670.17959306577192755No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT1650.17744225061298233No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG1630.17529143545403708No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1630.17529143545403708No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1610.17314062029509183No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1570.16883898997720137No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1550.1666881748182561No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1510.16238654450036566No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1510.16238654450036566No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1510.16238654450036566No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1490.16023572934142039No Hit
NAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1480.15916032176194778No Hit
CAGGAANNNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1470.15808491418247517No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1450.15593409902352992No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1430.15378328386458467No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT1420.15270787628511207No Hit
CAGGAAANAGCTATGANNTGCAATCTTGAGCTCCTGGGCTCAGGTGATCT1410.15163246870563943No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1400.15055706112616682No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1390.1494816535466942No Hit
CAGGAAANNGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1370.14733083838774894No Hit
CAGGAAANNGCTATGANNCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG1360.14625543080827633No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1350.14518002322880372No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1340.1441046156493311No Hit
CAGGAAANNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1340.1441046156493311No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1330.1430292080698585No Hit
NAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1320.14195380049038586No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1310.14087839291091322No Hit
CAGGAAACAGCNATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1300.13980298533144062No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1290.138727577751968No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1290.138727577751968No Hit
CAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1280.13765217017249537No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1280.13765217017249537No Hit
NAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1280.13765217017249537No Hit
CAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1270.13657676259302276No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1270.13657676259302276No Hit
CAGGAAANAGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC1270.13657676259302276No Hit
CAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1260.13550135501355012No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1260.13550135501355012No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT1260.13550135501355012No Hit
NAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1240.1333505398546049No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC1220.13119972469565966No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1220.13119972469565966No Hit
NAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1210.13012431711618705No Hit
CAGGAAANAGCTATGANNTTGGACAGCACCTTGAATGCTTTCAGGGAAGC1190.12797350195724178No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1180.12689809437776917No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1160.12474727921882393No Hit
CAGGAAANNGCTATGANNATTAGCCAGGTGTGGTGGCGGGCGCATCCACC1130.12152105648040606No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1120.12044564890093346No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1120.12044564890093346No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1120.12044564890093346No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1110.11937024132146085No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1100.11829483374198821No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1090.11721942616251559No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1020.10969157310620727No Hit
CAGGAAANAGCTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC1000.107540757947262No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1000.107540757947262No Hit
NAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC960.10323912762937153No Hit
CAGGAAACAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAACT950.1021637200498989No Hit
NAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC950.1021637200498989No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT950.1021637200498989No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCATGGGCAACAG940.1010883124704263No Hit
NAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA940.1010883124704263No Hit
CAGGAAANNGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG930.10001290489095369No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT930.10001290489095369No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)