FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72786
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC22103.036298189212211No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG20892.8700574286263842No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC18172.496359189954112No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT16442.25867611903388No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG16082.2092160580331384No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC15592.141895419448795No Hit
TGTAAAACGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG14161.9454290660291813No Hit
TGTAAAACGACGGCCAGTAGGAGGGCGGAGGAGGAAAGCAGGTTTGCTCT13551.8616217404445907No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC13521.857500068694529No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC12971.7819360866100624No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC12811.7599538372763994No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT12641.7365976973593822No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC11991.647294809441376No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC11131.529140219272937No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC10991.509905751105982No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA10871.4934190641057346No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT10561.4508284560217624No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG10421.4315939878548072No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA9571.3148132882697223No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG9341.2832138048525814No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG9021.2392493061852554No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA8491.1664331052674966No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG8261.1348336218503559No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATCTGGGCACTGGA8181.1238424971835244No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC7951.0922430137663837No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA7721.060643530349243No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA7701.0578957491825352No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG7411.0180529222652708No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA7411.0180529222652708No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC7391.015305141098563No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA7361.0111834693485011No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC7331.0070617975984393No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA7230.9933228917648999No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC7160.9837056576814222No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC6850.9411150495974501No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT6650.9136372379303712No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA6540.8985244415134779No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT6140.8435688181793202No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA6020.827082131179073No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT5890.8092215535954717No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG5830.800978210095348No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG5700.7831176325117468No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC5620.7721265078449152No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT5520.7583876020113759No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC5450.7487703679278982No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG5220.7171708845107576No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT4500.6182507625092737No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA4370.6003901849256725No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT4300.5907729508421949No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC3980.5468084521748688No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA3790.5207045310911439No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT3520.48360948534058745No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA2800.3846893633391037No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT2690.3695765669222103No Hit
TGTAAAACGACGGCCAGTTTTCCCCTTCTCCCTCCAGGGGTGCTTTCTCT2650.36408100458879455No Hit
TGTAAAACGACGGCCAGTTGCTATATGTCGTCCTCTGGAAATGCCATTTA2580.35446377050531697No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTGCCTGAAAGAAG2530.34759431758854725No Hit
TGTAAAACGACGGCCAGTGGGGACAAGGGTGGCTTTGTAAATAGCAGCAA2490.3420987552551315No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG2470.33935097408842363No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG2420.3324815211716539No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCACCTGATAATAA2390.3283598494215921No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2360.32423817767153024No Hit
TGTAAAACGACGGCCAGTTGCCCATCAGCAATCCTGCCAGGAGAGCACAA2090.28714313192097385No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGTATTTTGCATTGTCACA1760.24180474267029373No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTTAATACATTAGT1530.2102052592531531No Hit
TGTAAAACGACGGCCAGTAGCACAGCTGCCCTTGGATCGACATGGAGAAA1510.2074574780864452No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGACGCAGCCCGTT1500.20608358750309122No Hit
TGTAAAACGACGGCCAGTTGCCTTGAAGATTTTCTGTAGGATAAAAGGTG1490.20470969691973728No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTCCTCTATGGGCT1420.19509246283625972No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTTGGCCTCC1310.17997966641936636No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTCCTCTCTGGGCT1300.17860577583601242No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC1230.16898854175253483No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC1040.14288462066880991No Hit
TGTAAAACGACGGCCAGTCTGCTGCTGCCCACTGAGATTTCTAAGCAGTA1000.13738905833539417No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCCCTCTGTCCTAT970.13326738658533235No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCACGTGTCGCCC940.12914571483527051No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTCTGTATCAAA870.11952848075179293No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTCGGCCTCC850.11678069958508505No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGCA840.1154068090017311No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG800.10991124666831535No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCAGCCCGAGACCT790.10853735608496139No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGCCGCCCGCTGC790.10853735608496139No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)