FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136226
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT34932.56412138651946No Hit
TGTAAAANGACGGCCAGTTCCCACCACCTGCCTTCCGGTCATAGCTGTTT34362.5222791537591944No Hit
TGTAAAACGACGGCCAGTTCCCACCACCTGCCTTCCGGTCATAGCTGTTT33752.4775006239631203No Hit
TGTAAAACGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT33692.473096178409408No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA30102.209563519445627No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC29982.2007546283382027No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA29362.1552420242831762No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC26081.914465667346909No Hit
TGTAAAANGACGGCCAGTTCCTCCTTTGCCAATATAGGGTTTGATGTAAT23021.689838944107586No Hit
TGTAAAACGACGGCCAGTTCCTCCTTTGCCAATATAGGGTTTGATGTAAT19481.4299766564385652No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG18411.3514307107306975No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG18241.338951448328513No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGTTGGGAGTGGTCATAGCT11500.8441853977948409No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGTGGTCATAGCT11350.8331742839105604No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT10570.7759164917123016No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC10310.7568305609795487No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT9670.7098498084066185No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC9050.6643372043515923No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG7980.5857912586437244No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGGTCATAGCTGTT7640.5608327338393552No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTGGTCAT7620.5593645853214512No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTGGTCAT7310.5366082832939381No Hit
TGTAAAANGACGGCCAGTCAGTGTCCGCAGAAGCAAGGGTCATAGCTGTT7300.5358742090349861No Hit
TGTAAAANGACGGCCAGTTCTCTGTGACTTGCAAAAGTCAGGTCATAGCT7240.5314697634812737No Hit
TGTAAAACGACGGCCAGTTCTCTGTGACTTGCAAAAGTCAGGTCATAGCT7210.5292675407044176No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTATTGGTCTCTTGCTGCGGTCATA7170.5263312436686095No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGGTGGGGTGTCGTGGGTCA7090.5204586495969933No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG6870.5043090159000485No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTATTGGTCTCTTGCTGCGGTCATA6820.5006386446052883No Hit
TGTAAAANGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA6770.49696827331052806No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT6750.495500124792624No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTCCGGTCATAGCT6650.48815938220310373No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTCCGGTCATAGCT6500.4771482683188231No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGGTGGGGTGTCGTGGGTCA6310.46320085739873446No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC6170.45292381777340596No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA5980.4389764068533173No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT5940.4360401098175091No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT5830.42796529296903674No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC5730.42062455037951635No Hit
TGTAAAANGACGGCCAGTGTGGTAACTGCTGCAGGGCTGAGGGTCATAGC5130.37658009484239424No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTGAGGGTCATAGC5100.37437787206553813No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCCCCTCTCCCAGCAGGTCATAG5050.37070750077077796No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT5030.3692393522528739No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC4980.3655689809581137No Hit
TGTAAAANGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA4940.3626326839223056No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG4850.3560260155917373No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG4830.3545578670738332No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC4770.35015342152012097No Hit
TGTAAAANGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC4690.3442808274485047No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC4450.32666304523365586No Hit
TGTAAAANGACGGCCAGTGGCTCTCATCCCCCTCTCCCAGCAGGTCATAG4440.3259289709747038No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA4390.32225859967994364No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG4310.31638600560832736No Hit
TGTAAAANGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG4160.3053748917240468No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG4130.3031726689471907No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC4110.30170452042928664No Hit
TGTAAAANGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG3940.28922525802710203No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG3910.2870230352502459No Hit
TGTAAAANGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG3800.27894821840177353No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC3640.267203030258541No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG3620.26573488174063686No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG3570.2620645104458767No Hit
TGTAAAANGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC3410.25031932230264414No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG3240.23784005990045953No Hit
TGTAAAANGACGGCCAGTGGGCAGGAGAGGCAGCTAACGGGTCATAGCTG3230.2371059856415075No Hit
TGTAAAACGACGGCCAGTTGGAGGTGGGGCCTGATGGGTCATAGCTGTTT3200.23490376286465137No Hit
TGTAAAACGACGGCCAGTGGGCAGGAGAGGCAGCTAACGGGTCATAGCTG3200.23490376286465137No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG3180.2334356143467473No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT3120.2290311687930351No Hit
TGTAAAANGACGGCCAGTTGGAGGTGGGGCCTGATGGGTCATAGCTGTTT2900.2128815350960903No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT2760.20260449547076184No Hit
TGTAAAANGACGGCCAGTTCCTTCCTGGGTGGGACGTTGCTACCGGTCAT2710.19893412417600165No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA2570.18865708455067315No Hit
TGTAAAACGACGGCCAGTTCCTTCCTGGGTGGGACGTTGCTACCGGTCAT2460.18058226770220076No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC2430.17838004492534465No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA2430.17838004492534465No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCATAGCTGTTTCCTGC2420.17764597066639262No Hit
TGTAAAANGACGGCCAGTTCCTCCTTTGCTTTGGGGCCCAGGGTCATAGC2380.17470967363058448No Hit
TGTAAAACGACGGCCAGTTCCTCCTTTGCTTTGGGGCCCAGGGTCATAGC2370.17397559937163246No Hit
TGTAAAANGACGGCCAGTATCTGCACATCGTGCTGCCACACGAGGCTTCA2360.1732415251126804No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCATAGCTGTTTCCTGC2310.16957115381792023No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGTTCACTGCAACC2130.1563578171567836No Hit
TGTAAAACGACGGCCAGTATCTGCACATCGTGCTGCCACACGAGGCTTCA2130.1563578171567836No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGTTCACTGCAACC2060.15121929734411935No Hit
TGTAAAANGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT1980.14534670327250304No Hit
TGTAAAANGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG1890.1387400349419347No Hit
TGTAAAANGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT1870.13727188642403065No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG1860.13653781216507863No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG1800.1321333666113664No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCATTGCAACC1790.13139929235241435No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGGTGTCGTGGGTCA1790.13139929235241435No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACT1770.12993114383451032No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGGGGCCTGATGGGT1700.12479262402184604No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT1690.12405854976289402No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTACAACC1680.12332447550394197No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGAGCTGAGGGTCATAGC1660.1218563269860379No Hit
TGTAAAANGACGGCCAGTGTGGTAACTGCTGCAGAGCTGAGGGTCATAGC1660.1218563269860379No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACCACAACC1650.12112225272708588No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT1640.12038817846813384No Hit
TGTAAAANGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGTGGGCGCCTG1630.11965410420918181No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG1610.11818595569127774No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACT1610.11818595569127774No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAATC1600.11745188143232568No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTACAACC1590.11671780717337366No Hit
TGTAAAANGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCACCTG1590.11671780717337366No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT1560.11451558439651754No Hit
TGTAAAACGACGGCCAGTAGCCCCTCATCCCCCTCTCCCAGCAGGTCATA1530.11231336161966145No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC1530.11231336161966145No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC1520.1115792873607094No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGTTGGGGTGTCGTGGGTCA1510.11084521310175738No Hit
TGTAAAANGACGGCCAGTTCCTCCTTGCCCTGTCACTGCGGTCATAGCTG1500.11011113884280532No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGGCCTGATGGGT1500.11011113884280532No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTTACTGCAACC1480.10864299032490128No Hit
TGTAAAANGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA1470.10790891606594923No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGCCTGATGGGTC1460.10717484180699721No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCACCTG1460.10717484180699721No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTAACC1450.10644076754804516No Hit
TGTAAAANGACGGCCAGTAGCCCCTCATCCCCCTCTCCCAGCAGGTCATA1430.10497261903014109No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC1430.10497261903014109No Hit
TGTAAAACGACGGCCAGTTCCTCCTTGCCCTGTCACTGCGGTCATAGCTG1420.10423854477118906No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAATC1420.10423854477118906No Hit
TGTAAAANGACGGCCAGTAGCCCCTCGTCACCTACTCCCAGCAGGTCATA1420.10423854477118906No Hit
TGTAAAANGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC1420.10423854477118906No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACCGCAACC1410.10350447051223702No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGTTGGGATGTCGTGGGTCA1380.10130224773538092No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCAGCAGGTCATA1380.10130224773538092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)