FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136226
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC25251.8535375038538897No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT22041.6178996667302863No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC20701.5195337160307136No Hit
CAGGAAANAGCTATGANNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT15391.129740284527183No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC14871.091568423061677No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT14771.0842276804721565No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG13130.9638395020040227No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC12820.9410831999765097No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG12360.9073157840647159No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT9780.7179246252550908No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC9500.6973705460044338No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTGGCCGTC9340.6856253578612013No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT8990.6599327587978799No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC8480.6224949715913262No Hit
CAGGAAANAGCTATGANCGCAGCAAGAGACCCAGACCCACCCGACTGGCC8440.619558674555518No Hit
CAGGAAANAGCTATGANNACTCCCAACTCTTGGCTGTGCCACTGGCCGTC8190.6012068180817172No Hit
CAGGAAANAGCTATGACCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT7810.5733119962415398No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG7710.5659712536520195No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC7420.5446831001424104No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG6760.49623419905157606No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC6730.49403197627472No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT6280.46099863462187834No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC6060.44484900092493357No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC5840.42869936722798885No Hit
CAGGAAANAGCTATGACCACTCCCAACTCTTGGCTGTGCCACTGGCCGTC5520.4052089909415237No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC5490.4030067681646676No Hit
CAGGAAACAGCTATGACCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT5350.392729728539339No Hit
CAGGAAANAGCTATGANCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG5330.391261580021435No Hit
CAGGAAANAGCTATGANCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT5270.38685713446772274No Hit
CAGGAAANAGCTATGANNTGACTTTTGCAAGTCACAGAGAACTGGCCGTC5200.3817186146550585No Hit
CAGGAAANAGCTATGANNAGGACTTGCTCTGGATTTCCATTTGTACTGGC5110.3751119463244902No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCCACCCGACTGGCC4980.3655689809581137No Hit
CAGGAAANAGCTATGANNCTTGCTTCTGCGGACACTGACTGGCCGTCGTT4960.36410083244020963No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGCTGTGCCACTGG4930.3618986096633536No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG4850.3560260155917373No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT4620.33914230763584047No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG4570.33547193634108025No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA4520.3318015650463201No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4500.330333416528416No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC4490.32959934226946397No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4190.3075771145009029No Hit
CAGGAAANAGCTATGACCCTTGCTTCTGCGGACACTGACTGGCCGTCGTT4140.30390674320614275No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC4020.2950978520987183No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC3930.28849118376815No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC3690.27087340155330114No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3650.26793710451749303No Hit
CAGGAAANAGCTATGANNCTCAGCCCTGCAGCAGTTACCACACTGGCCGT3610.26500080748168486No Hit
CAGGAAACAGCTATGACCACTCCCAACTCTTGGCTGTGCCACTGGCCGTC3560.26133043618692464No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC3490.2561919163742604No Hit
CAGGAAANAGCTATGANCACTCCCAACTCTTGGCTGTGCCACTGGCCGTC3390.24885117378474006No Hit
CAGGAAANAGCTATGACCTGACTTTTGCAAGTCACAGAGAACTGGCCGTC3280.24077635693626767No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3270.24004228267731562No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3250.23857413415941156No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC3240.23784005990045953No Hit
CAGGAAANAGCTATGACCCACGACACCCCACCTCTTGGCTGTGCCACTGG3180.2334356143467473No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3170.23270154008779528No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCAATACCCACCCGACTGGCC3140.23049931731093917No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC3110.2282970945340831No Hit
CAGGAAANAGCTATGACCAGGACTTGCTCTGGATTTCCATTTGTACTGGC3100.22756302027513103No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGAGCCACTGGCCG3080.22609487175722695No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC2970.21802005490875456No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2930.21508375787294642No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2730.2004022726939057No Hit
CAGGAAANAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2680.19673190139914554No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2630.19306153010438537No Hit
CAGGAAANAGCTATGANCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2600.19085930732752926No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA2550.18718893603276907No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2550.18718893603276907No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2550.18718893603276907No Hit
CAGGAAANAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG2490.18278449047905684No Hit
CAGGAAANAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2420.17764597066639262No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2290.16810300530001615No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2290.16810300530001615No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCCACTGGCCGTCG2290.16810300530001615No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC2280.16736893104106412No Hit
CAGGAAACAGCTATGANCTTCATACAGAGATACAAAGGCAACTGGCCGTC2260.16590078252316004No Hit
CAGGAAANAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2240.16443263400525596No Hit
CAGGAAANAGCTATGACCCTCAGCCCTGCAGCAGTTACCACACTGGCCGT2200.16149633696944782No Hit
CAGGAAANAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC2180.16002818845154376No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCCACCCGACTGGCC2130.1563578171567836No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2100.15415559437992746No Hit
CAGGAAANAGCTATGACCTGCTGGGAGAGGGGGATGAGAGCCACTGGCCG2060.15121929734411935No Hit
CAGGAAANAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2050.1504852230851673No Hit
CAGGAAACAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2030.14901707456726324No Hit
CAGGAAACAGCTATGANCGCAGCAAGAGACCCAGACCCACCCGACTGGCC2010.14754892604935915No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2010.14754892604935915No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2010.14754892604935915No Hit
CAGGAAANAGCTATGANCTGACTTTTGCAAGTCACAGAGAACTGGCCGTC2010.14754892604935915No Hit
CAGGAAANAGCTATGANNCATCAGGCCCCACCTCCAACTGGCCGTCGTTT2010.14754892604935915No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1990.14608077753145507No Hit
CAGGAAACAGCTATGACCCTTGCTTCTGCGGACACTGACTGGCCGTCGTT1980.14534670327250304No Hit
CAGGAAACAGCTATGACCTGACTTTTGCAAGTCACAGAGAACTGGCCGTC1970.14461262901355101No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG1960.143878554754599No Hit
CAGGAAANAGCTATGANCAGGACTTGCTCTGGATTTCCATTTGTACTGGC1940.1424104062366949No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1930.14167633197774288No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGGAAGGAACTGGC1920.14094225771879085No Hit
CAGGAAACAGCTATGACCAGGACTTGCTCTGGATTTCCATTTGTACTGGC1910.1402081834598388No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC1900.13947410920088676No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1900.13947410920088676No Hit
CAGGAAANAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1880.13800596068298268No Hit
CAGGAAANAGCTATGANCCACGACACCCCACCTCTTGGCTGTGCCACTGG1830.13433558938822251No Hit
CAGGAAANAGCTATGANCCTTGCTTCTGCGGACACTGACTGGCCGTCGTT1830.13433558938822251No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGCTGTGCCACTGG1820.1336015151292705No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1820.1336015151292705No Hit
CAGGAAANAGCTATGANCGCAGCAAGAGACCAATACCCACCCGACTGGCC1730.12699484679870215No Hit
CAGGAAANAGNTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC1710.1255266982807981No Hit
CAGGAAANAGCTATGANCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1690.12405854976289402No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC1670.12259040124498995No Hit
NAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT1660.1218563269860379No Hit
CAGGAAANAGCTATGANNACTGCCCACACCAACTGGCCGTCGTTTTACAC1640.12038817846813384No Hit
CAGGAAANAGCTATGANCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1640.12038817846813384No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGT1610.11818595569127774No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1600.11745188143232568No Hit
CAGGAAANAGCTATGANCCTCAGCCCTGCAGCAGTTACCACACTGGCCGT1570.11524965865546959No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1570.11524965865546959No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1540.11304743587861348No Hit
CAGGAAANAGCTATGANNCTGGGCCCCAAAGCAAAGGAGGAACTGGCCGT1500.11011113884280532No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1490.10937706458385331No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1490.10937706458385331No Hit
NAGGAAANAGNTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC1470.10790891606594923No Hit
CAGGAAANAGCTATGANNACCCCACCTGCTCAAAAGACACACTCCTAAAA1460.10717484180699721No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCAG1450.10644076754804516No Hit
CAGGAAANAGCTATGANCTGCTGGGAGAGGGGGATGAGAGCCACTGGCCG1450.10644076754804516No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1440.10570669328909313No Hit
NAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC1440.10570669328909313No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA1430.10497261903014109No Hit
CAGGAAACAGCTATGACCCTCAGCCCTGCAGCAGTTACCACACTGGCCGT1420.10423854477118906No Hit
CAGGAAANAGNTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC1410.10350447051223702No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGAGCCACTGGCCG1370.10056817347642887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)