FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148393
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA25171.6961716523016583No Hit
TGTAAAANGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT23041.5526338843476444No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA22711.5303956386082902No Hit
TGTAAAANGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT22621.5243306624975572No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT21321.4367254520091919No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG20641.3909011880614315No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT20101.3545113313970336No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC19861.3383380617684122No Hit
TGTAAAANGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA19801.334294744361257No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA19791.333620858126731No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT19741.3302514269541015No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG19711.328229768250524No Hit
TGTAAAANGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA19271.2985787739313848No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA18861.2709494383158235No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG18831.2689277796122458No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC18171.2244512881335374No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC18111.220407970726382No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG17731.1948002938143982No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC17561.1833442278274582No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT17541.1819964553584064No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA17301.1658231857297852No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA17061.1496499161011637No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT14911.004764375678098No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT13350.8996381230920596No Hit
TGTAAAANGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC12530.8443794518609369No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC11940.8046201640239096No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC11870.7999029603822283No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG11340.7641869899523562No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC11320.7628392174833045No Hit
TGTAAAANGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG11240.7574481276070973No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG10830.729818791991536No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC10290.6934289353271381No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG9880.6657995997115767No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT9540.6428874677376966No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT9010.6071714973078245No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC9010.6071714973078245No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT8970.604475952369721No Hit
TGTAAAANGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA8850.5963893175554102No Hit
TGTAAAANGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC8720.5876287965065737No Hit
TGTAAAANGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC8460.5701077544089007No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC8400.5660644370017454No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA8350.5626950058291159No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT8100.5458478499659688No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC7870.5303484665718733No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG7850.5290006941028216No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC7680.5175446281158815No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG7490.5047407896598897No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG7230.4872197475622166No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG7100.47845922651338No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC7080.4771114540443282No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC6750.45487320830497396No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC6730.45352543583592214No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA6600.4447649147870857No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC6400.4312871900965679No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC6330.4265699864548867No Hit
TGTAAAANGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA6320.42589610022036084No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC6260.4218527828132055No Hit
TGTAAAANGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC6230.4198311241096278No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA6150.41444003423342074No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC6150.41444003423342074No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT5900.3975928783702736No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC5820.3922017884940664No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC5720.38546292614880756No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC5510.3713113152237639No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC5370.3618769079404015No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA5360.3612030217058756No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC5130.3457036383117802No Hit
TGTAAAANGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA4840.3261609375105295No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4810.3241392788069518No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT4760.32076984763432237No Hit
TGTAAAANGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC4710.3174004164616929No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4640.3126832128200117No Hit
TGTAAAANGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG4340.2924666257842351No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA4300.28977108084613157No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA4300.28977108084613157No Hit
TGTAAAANGACGGCCAGTGCCAATCCTGAGGCCATCAATAGCTATCTGTT4190.2823583322663468No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA4180.2816844460318209No Hit
TGTAAAANGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG4060.27359781121751026No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC4030.2715761525139326No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAATAGCTATCTGTT3930.26483729016867374No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3820.25742454158888894No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG3800.2560767691198372No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC3740.2520334517126819No Hit
TGTAAAANGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG3570.24057738572574178No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG3560.23990349949121592No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3540.2385557270221641No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG3530.23788184078763824No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3510.23653406831858648No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG3460.23316463714595703No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC3430.23114297844237935No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3320.2237302298625946No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG3300.22238245739354284No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA3170.21362193634470628No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC3160.21294805011018042No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC3150.2122741638756545No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG3150.2122741638756545No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG3030.20418752906134385No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA2900.19542700801250734No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG2730.18397094202556724No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT2690.1812753970874637No Hit
TGTAAAANGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC2550.17184098980410129No Hit
TGTAAAANGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT2440.1644282412243165No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT2440.1644282412243165No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT2360.15903715134810942No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2240.15095051653379876No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG2220.149602744064747No Hit
TGTAAAACGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC2170.14623331289211755No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG2160.14555942665759167No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG2140.1442116541885399No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA2060.1388205643123328No Hit
TGTAAAANGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA2050.1381466780778069No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAAGGACTGGCTGGC2020.13612501937422924No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT1930.13006004326349627No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC1910.12871227079444447No Hit
TGTAAAANGACGGCCAGTGCCAATCCTGAGGCCATCAAGGACTGGCTGGC1880.12669061209086682No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG1860.12534283962181506No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1860.12534283962181506No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG1820.1226472946837115No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCCCAGCTTCT1820.1226472946837115No Hit
TGTAAAANGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT1790.12062563598013384No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGCA1710.11523454610392675No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC1680.11321288740034907No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCCCAGCTTCT1660.1118651149312973No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGCA1630.10984345622771963No Hit
TGTAAAANGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACATAAATA1590.1071479112896161No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA1540.10377848011698665No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT1520.10243070764793488No Hit
TGTAAAANGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT1520.10243070764793488No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACATAAATA1510.101756821413409No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT1510.101756821413409No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC1500.10108293517888309No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)