FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158848
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC41972.642148468976632No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG40702.5621978243352133No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA38452.4205529814665594No Hit
CAGGAAACAGCTATGACCAGCCTCTCCCCTCGTTCCAGCAACTCAGTGAG34932.198957493956487No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT34752.1876259065269945No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA33802.1278203062046734No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC30371.9118906124093473No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA26161.6468573730862208No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG25101.5801269137792104No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG24451.5392072925060436No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC24001.5108783239323127No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA23611.4863265511684127No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT22021.386230862207897No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA21591.3591609589041096No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC21071.326425261885576No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG19651.237031627719581No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT17511.1023116438356164No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG16951.0670578162771958No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG16921.0651692183722803No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC16601.045024174053183No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG16261.0236200644641418No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC15150.9537419419822724No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG14840.9342264302981466No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG14480.911563255439162No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA14300.9002316680096696No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA14210.8945658742949234No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT14190.8933068090249798No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC13880.8737912973408541No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG12960.8158742949234488No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG12850.808949435938759No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC12670.7976178485092668No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT12000.7554391619661563No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTGGCCGTCGTTT11890.7485143029814666No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA11840.7453666398066076No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC11780.7415894439967768No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGGCCGGGGTATGG10700.6735999194198228No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTGCTTGTCATT10350.6515662771958098No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA10300.6484186140209508No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGTATTCAAGATTC10170.6402346897663175No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG10130.6377165592264303No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG9980.6282735697018533No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC9830.6188305801772763No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG9590.6037217969379532No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT9380.5905016116035455No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTCACATCTATAAT9360.589242546333602No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCGTGTGCGCT9340.5879834810636584No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCAAAATTATCCT9330.5873539484286866No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG9320.5867244157937147No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG9170.5772814262691378No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA9000.5665793714746172No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTGCCGTGTGCGC8890.5596545124899275No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC8570.53950946817083No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA8460.5325846091861401No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG8110.5105509669621273No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGGGGAGGGTGG7710.48536966156325545No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA7670.48285153102336825No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGAGGCCAGAGGT7570.4765562046736503No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT7390.465224617244158No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGGCCGTCGTTT7370.46396555197421435No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCATCTGCCCCTT7360.46333601933924257No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC7130.44885676873489117No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA6890.4337479854955681No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCATGCCTGTAA6610.4161210717163578No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTAACAAACTCCC6320.3978646253021757No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA6210.3909397663174859No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG6130.3859035052377115No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG6030.37960817888799353No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT6000.3777195809830782No Hit
CAGGAAACAGCTATGACCTCAGTTTCTTGTTTTCGTGATACTTCGCTGCA5730.36072219983883963No Hit
CAGGAAACAGCTATGACCTCTACGCCCCTCAGCTGTGTCCTGTCTGCATA5720.36009266720386784No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG5450.3430952860596293No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCACCCAGCACAGG5260.33113416599516515No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCCGCC5060.3185435132957293No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT5020.3160253827558421No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA4840.3046937953263497No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGTCAGTCCCCATC4680.294621273166801No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCGCTCTCCTCCC4540.2858078162771958No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG4480.28203062046736505No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4410.27762389202256244No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT4370.2751057614826753No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCGTGTGCGCTGG4360.27447622884770345No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCAGTGGAAGG4320.27195809830781625No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTTCACATATAC4250.2675513698630137No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCGCAGTGGGGCCA4130.2599969782433521No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC4130.2599969782433521No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC4010.25244258662369057No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG3920.2467767929089444No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTTGTGCTGGGCCA3910.2461472602739726No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT3810.23985193392425463No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCACTCAGCGTGTG3510.22096595487510073No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT3480.21907735697018535No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT3390.21341156325543914No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTTTTACACCA3270.2058571716357776No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC3220.2027095084609186No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCAGCGTGGAGGG3170.19956184528605964No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC3140.19767324738114425No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA3140.19767324738114425No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGACGTGTCCGCT3060.19263698630136986No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG3030.19074838839645447No Hit
CAGGAAACAGCTATGACCGAGGCGCAGGTCACAGTGAGACCAATACAGAG2930.1844530620467365No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG2790.17563960515713134No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC2740.17249194198227236No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA2690.16934427880741337No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCTTTTTAGATGAG2650.16682614826752618No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA2620.16493755036261082No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT2610.164308017727639No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCCTGGGTATGTGG2580.16241941982272362No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCCTGGCTTGTCC2470.15549456083803384No Hit
CAGGAAACAGCTATGACCAACTTTTAGGTAGAGCCAAAGAACTGGCCGTC2460.15486502820306203No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGCCCAGCCACAG2340.1473106365834005No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGACAGGAAACA2290.14416297340854148No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCCGATTC2290.14416297340854148No Hit
CAGGAAACAGCTATGACCAACACCCTTGGCCTCCCTCACTGGCCGTCGTT2260.14227437550362612No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCAGAGGGCGGCCA2180.13723811442385173No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGAGGAGCCGGT2170.13660858178887994No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGACGCTGCCCTGC2140.13471998388396453No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAACAGGAAACAGAA2130.13409045124899274No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCTGTCTCCTGAGA2120.13346091861402093No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCCCCTGCCTACC2030.12779512489927478No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTGCCATGCTCACC2010.1265360596293312No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCGCCTGTCACC1970.12401792908944399No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGACAGAAGCCCA1700.10702054794520548No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTAGGCCA1680.10576148267526189No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)