FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148212
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG78205.276225946617008No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC71334.812700725987099No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG63744.300596442933096No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC57523.88092732032494No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG39632.673872560926241No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG36382.454592070817478No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG35972.426928993603757No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA34602.334493833157909No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA27701.8689444849269967No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC24531.6550616684209107No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG23981.6179526624025047No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGAGCTCGGC20321.3710090950800204No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA18901.2752003886324994No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC17591.1868134833886594No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC14120.952689390872534No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC13040.8798207972363911No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT12890.8697001592313713No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC12720.8582301028256821No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC12590.8494588832213317No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT12240.8258440612096187No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG11710.7900844735918819No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG11170.7536501767738105No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG10630.7172158799557391No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC9970.6726850727336517No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA9650.6510943783229428No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC9230.6227565919088873No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG9010.6079129895015248No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA9000.6072382803011902No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG8850.5971176422961704No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG8770.5917199686934932No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG8540.576201657085796No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA8330.5620327638787682No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC8170.5512374166734137No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC8130.5485385798720752No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC7790.5255984670606968No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG7110.4797182414379403No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC6460.4358621434161877No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA6200.4183197042074866No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG5990.4041508110004588No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA5750.38795779019242704No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT5000.3373546001673279No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT4680.3157639057566189No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT4630.3123903597549456No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC4590.30969152295360697No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC4470.30159501254959115No Hit
TGTAAAACGACGGCCAGTTCAGGGAGGCGGGGCTCGTTCCACAGGGACAC3970.2678595525328583No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA3700.24964240412382266No Hit
TGTAAAACGACGGCCAGTTCAGGGAGGCGGGGCTCGTGCCACAGGGACAC3670.24761827652281868No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT3550.23952176611880277No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA3330.22467816371144037No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT3170.21388281650608587No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG2740.1848703208916957No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG2630.17744851968801448No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG2590.17474968288667586No Hit
TGTAAAACGACGGCTAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG2330.15720724367797478No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGCCTGGGCCAGAGCCTGG2040.13764067686826978No Hit
TGTAAAACGACGGCTAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC2010.1356165492672658No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCTGGGTGGGAC2000.13494184006693116No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG1990.1342671308665965No Hit
TGTAAAACGACGGCTAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC1950.13156829406525788No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA1920.1295441664642539No Hit
TGNAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG1890.12752003886324995No Hit
TGTAAAACGACGGCTAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG1860.12549591126224596No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACCGTGAGACGGGCACC1810.12212236526057269No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT1730.11672469165789545No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAACCCCGGTCGGGTTCTGG1690.11402585485655684No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCCCAGGGGTGCTGGAGGC1680.11335114565622216No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTTGAATTCC1680.11335114565622216No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACTGGGTGGGACG1640.11065230885488354No Hit
TGNAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGCGAGACGGGCACC1640.11065230885488354No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1630.10997759965454888No Hit
TGNAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG1570.10592934445254096No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAAAGTGACAGGTAG1560.10525463525220631No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGACTGTGAGGCCCTG1540.10390521685153697No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCCGGGGTGCTGGAGGC1490.10053167084986371No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)