FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148212
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC36272.4471702696137965No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT32562.1968531562896394No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC29852.0140069629989474No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC27141.831160769708256No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG24741.6692305616279384No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT14170.9560629368742073No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC13100.8838690524383991No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC11480.7745661619841848No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA10690.721264135157747No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG10310.6956251855450301No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG10190.6875286751410142No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC9920.6693115267319786No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG9480.6396243219172536No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG9350.6308531023129031No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG8500.5735028202844574No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT8450.5701292742827841No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA7990.53909265106739No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG7700.519526084257685No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG7670.517501956656681No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT7480.5046824818503225No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT6990.4716217310339244No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG6980.4709470218335897No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG6650.44868161822254604No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG6090.4108979030038053No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG5860.39537959139610823No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5850.3947048821957736No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA5710.3852589533910884No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT5550.37446360618573393No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT5090.3434269829703398No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT4980.33600518176665856No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG4180.2820284457398861No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT4110.2773054813375435No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4010.27055838933419696No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3870.26111246052951176No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG3840.2590883329285078No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3790.2557147869268345No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3780.2550400777264999No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3670.24761827652281868No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3650.24626885812214935No Hit
NAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3580.24154589371980675No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG3540.23884705691846814No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3500.23614822011712952No Hit
CAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3410.23007583731411763No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC3400.22940112811378294No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3350.2260275821121097No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT3340.22535287291177505No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA3230.21793107170809384No Hit
CAGGAAANNGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG3230.21793107170809384No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG3220.21725636250775915No Hit
NAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3200.21590694410708985No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT3010.20308746930073138No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG2850.19229212209537688No Hit
NAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2850.19229212209537688No Hit
CAGGAAANNGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2840.19161741289504222No Hit
NAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2810.18959328529403827No Hit
NAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2720.18352090249102637No Hit
CAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2670.1801473564893531No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2560.17272555528567188No Hit
CAGGAAANAGCTATGANNCGGGACCCAAAGTTCTGCGTGATGGTCAGCAC2560.17272555528567188No Hit
CAGGAAANNGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT2480.16732788168299464No Hit
CAGGAAANNGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2470.16665317248265998No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT2470.16665317248265998No Hit
NAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2450.16530375408199066No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2440.164629044881656No Hit
CAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2430.16395433568132137No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2410.16260491728065204No Hit
CAGGAAANNGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2370.1599060804793134No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2350.1585566620786441No Hit
CAGGAAANAGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC2310.1558578252773055No Hit
NAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2300.15518311607697083No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2180.14708660567295495No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2170.1464118964726203No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2150.145062478071951No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2120.14303835047094704No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2080.1403395136696084No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2080.1403395136696084No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2070.13966480446927373No Hit
CAGGAAACAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2000.13494184006693116No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1980.13359242166626184No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1980.13359242166626184No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA1980.13359242166626184No Hit
CAGGAAANNGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1960.13224300326559255No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1940.13089358486492322No Hit
CAGGAANNNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1940.13089358486492322No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1920.1295441664642539No Hit
CAGGAAACAGCNATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1910.12886945726391924No Hit
CAGGAAACNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1870.12617062046258062No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTATTCCCAACCA1870.12617062046258062No Hit
CAGGAAACAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1840.12414649286157665No Hit
NAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1830.123471783661242No Hit
CAGGAAACNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1800.12144765606023804No Hit
CAGGAAANAGCTATGANNTGACTGCAAACATAGAAGTGAGCAGAGCAAGA1790.12077294685990338No Hit
NAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1790.12077294685990338No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1790.12077294685990338No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1780.12009823765956873No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1780.12009823765956873No Hit
NAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1770.11942352845923407No Hit
CAGGAAACNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1770.11942352845923407No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1740.11739940085823009No Hit
CAGGAANNNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1680.11335114565622216No Hit
NAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1660.11200172725555285No Hit
CAGGAANNNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1630.10997759965454888No Hit
CAGGAAACAGCNATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1620.10930289045421424No Hit
NAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1570.10592934445254096No Hit
CAGGAANNNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1560.10525463525220631No Hit
CAGGAAACNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1510.10188108925053302No Hit
NAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1490.10053167084986371No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)