FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130626
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT10430.798462786887756No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT8370.6407606448945845No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT8130.6223875798080014No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT7480.5726271951985057No Hit
NNGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT7110.5443020531900234No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC6590.5044937455024268No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT6580.5037282011238191No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT6460.49454166858052767No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA5800.44401573959242413No Hit
NNGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT5700.4363602958063479No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5520.4225804969914106No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT5440.41645614196254954No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT5430.41569059758394195No Hit
NNGGANNNNGNTATGNNNGCTTCTATATGAGCGCATCTCACCTGAAGTTG5430.41569059758394195No Hit
NNGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT4860.37205456800330716No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT4840.3705234792460919No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT4650.35597813605254697No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4580.35061932540229357No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4520.34602605913064777No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT4520.34602605913064777No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC4350.3330118046943181No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA4230.32382527215102663No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT3940.3016244851714054No Hit
NNGGANNNNGNTATGANNGCTTCTATATGAGCGCATCTCACCTGAAGTTG3840.2939690413853291No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3790.29014131949229094No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3760.2878446863564681No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT3750.28707914197786044No Hit
NAGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT3690.2824858757062147No Hit
NNGGANNNNGNTATGNNNCCCGTGACAGCTCCTGCAATTACAAAATGGCT3500.26794053251266975No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATTTTCATGT3480.2664094437554545No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3400.26028508872659345No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3220.24650528991165618No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT3200.2449742011544409No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT3200.2449742011544409No Hit
NNGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3190.2442086567758333No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT3110.2380843017469723No Hit
NAGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT3020.2311944023395036No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT2990.22889776920368074No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2910.22277341417481972No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2870.2197112366603892No Hit
NAGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2830.21664905914595872No Hit
NNGGANNNNGNTATGNNNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC2810.21511797038874345No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT2740.20975915973849005No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2680.2051658934668443No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2640.20210371595241378No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2630.20133817157380615No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2610.1998070828165909No Hit
NAGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT2590.19827599405937563No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2480.1898550058946917No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGACAGGAAACA2440.1867928283802612No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2430.18602728400165358No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2430.18602728400165358No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA2430.18602728400165358No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT2380.18219956210861543No Hit
NNGGANNNNGNTATGNNNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2350.17990292897279256No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2320.17760629583696966No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2320.17760629583696966No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA2310.17684075145836203No Hit
NNGGANNNNGNTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT2310.17684075145836203No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA2300.17607520707975444No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT2230.170716396429501No Hit
NNGGANNNNGNTATGNNNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG2220.1699508520508934No Hit
NAGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2210.16918530767228576No Hit
NNGGANNNNGNTATGNNNACAAACGCAAATGGCTTTTAGCATTTCACAGG2200.16841976329367814No Hit
NNGGANNNNGNTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC2170.16612313015785524No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT2150.16459204140064No Hit
NAGGANNNNGNTATGNNNGCTTCTATATGAGCGCATCTCACCTGAAGTTG2080.1592332307503866No Hit
NAGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT2080.1592332307503866No Hit
NAGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2070.15846768637177897No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2060.15770214199317134No Hit
NNGGANNNNGNTATGNNNAACCAACACTGCTGCTAATTGTGTTTGTGTCC2010.1538744201001332No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1960.15004669820709507No Hit
NAGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1960.15004669820709507No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1930.1477500650712722No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1900.1454534319354493No Hit
NNGGANNNNGNTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1900.1454534319354493No Hit
NNGGANNNNGNTATGNNNTGGCATCCAGGGCCTATTTGGTTCCTATTGAG1890.14468788755684167No Hit
NNGGANNNNGNTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1840.14086016566380355No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGACGCTGCCCTGC1830.1400946212851959No Hit
NNGGANNNNGNTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1810.13856353252798065No Hit
NAGGAAANAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1800.137797988149373No Hit
NAGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATTTTCATGT1800.137797988149373No Hit
NAGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1800.137797988149373No Hit
NNGGANNNNGNTATGNNNAAACTATTAATACCTGCAGCAAAGCCCCAAGC1790.1370324437707654No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1770.13550135501355012No Hit
NAGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1760.1347358106349425No Hit
NNGGANNNNGNTATGNNNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1760.1347358106349425No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1740.13320472187772725No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1730.13243917749911963No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGACAGGAAACA1720.131673633120512No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1710.13090808874190438No Hit
NNGGANNNNGNTATGANNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1700.13014254436329672No Hit
NAGGAANNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1680.12861145560608148No Hit
NAGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1680.12861145560608148No Hit
NNGGANNNNGNTATGNNNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1670.12784591122747385No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1660.12708036684886623No Hit
NNGGANNNNGNTATGNNNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1650.1263148224702586No Hit
NAGGANNNAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1640.12554927809165098No Hit
NNGGANNNNGNTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG1640.12554927809165098No Hit
NNGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1630.12478373371304335No Hit
NNGGANNNNGNTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1630.12478373371304335No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT1620.12401818933443572No Hit
NAGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1610.12325264495582809No Hit
NAGGANNNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1590.12172155619861284No Hit
NNGGANNNNGNTATGNNNGTCAACAGCATACCTGTACACCTGAGTGTTCC1570.12019046744139758No Hit
NNGGANNNNGNTATGANNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1560.11942492306278994No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1550.11865937868418232No Hit
NAGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1520.11636274554835944No Hit
NNGGAAANNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1500.11483165679114418No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1500.11483165679114418No Hit
NNGGANNNNGNTATGNNNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1500.11483165679114418No Hit
NNGGANNNNGNTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1490.11406611241253656No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1490.11406611241253656No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1490.11406611241253656No Hit
NNGGANNNNGNTATGANNGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1480.11330056803392893No Hit
NAGGANNNNGNTATGNNNCCCGTGACAGCTCCTGCAATTACAAAATGGCT1470.1125350236553213No Hit
NAGGAAANAGCTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1460.11176947927671367No Hit
NAGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1460.11176947927671367No Hit
NAGGANNNNGNTATGANNGCTTCTATATGAGCGCATCTCACCTGAAGTTG1460.11176947927671367No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1460.11176947927671367No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1450.11100393489810603No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGACGCTGCCCTGC1430.1094728461408908No Hit
NNGGANNNNGNTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1420.10870730176228316No Hit
NAGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1420.10870730176228316No Hit
NAGGAAANAGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1410.10794175738367554No Hit
NNGGANNNNGNTATGNNNTCCTCTTCATTTTCACGGTGCCATTCCGATTC1380.10564512424785265No Hit
NNGGANNNNGNTATGANNGTCAACAGCATACCTGTACACCTGAGTGTTCC1370.10487957986924502No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1350.10334849111202976No Hit
NNGGANNNNGNTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1330.10181740235481451No Hit
NAGGANNNAGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1310.10028631359759925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)