FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149238
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT11400.7638805130060708No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC11230.7524893123735242No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG10590.7096047923451132No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC10180.6821318967019123No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT9410.6305364585427303No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC9160.6137846929066324No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG8870.5943526447687586No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT8640.5789410203835484No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT8030.5380667122314692No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA7700.5159543815918197No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC7660.5132740990900441No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA7620.5105938165882684No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT7590.5085836047119366No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA7300.4891515565740629No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6510.4362159771639931No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG6420.43018534153499777No Hit
CAGGAAANAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT6370.42683498840777817No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA6180.41410364652434367No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC6110.4094131521462362No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC6070.40673286964446054No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG5840.3913212452592503No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA5630.37724976212492795No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA5500.368538843994157No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5260.3524571489835029No Hit
CAGGAAANAGCTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC5020.33637545397284874No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG4970.33302510084562914No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT4740.31761347646041893No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT4690.31426312333319933No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG4670.31292298208231145No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG4620.3095726289550919No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT4580.3068923464533162No Hit
CAGGAAANAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT4550.30488213457698443No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG4410.2955011458207695No Hit
CAGGAAANAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA4390.29416100456988165No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA4380.29349093394443776No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4380.29349093394443776No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4330.2901405808172181No Hit
CAGGAAANAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT4310.2888004395663303No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT4290.28746029831544245No Hit
CAGGAAANAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA4290.28746029831544245No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC4260.2854500864391107No Hit
CAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4120.2760690976828958No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG4060.27204867393023224No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4020.2693683914284566No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG4020.2693683914284566No Hit
CAGGAAANAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC4010.26869832080301265No Hit
CAGGAAANAGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT4000.2680282501775687No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3980.2666881089266809No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3950.2646778970503491No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3900.2613275439231295No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3880.2599874026722417No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA3760.2519465551669146No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3660.2452458489124754No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3620.24256556641069968No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT3540.2372050014071483No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC3500.23452471890537263No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3440.2305042951527091No Hit
CAGGAAANAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3380.22648387140004556No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3380.22648387140004556No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3330.22313351827282596No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC3270.21911309452016242No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT3240.21710288264383065No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3200.21442260014205497No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3180.21308245889116711No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG3170.2124123882657232No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT3160.2117423176402793No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT3110.20839196451305966No Hit
CAGGAAANAGCTATGANNAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT3070.20571168201128398No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA3060.20504161138584007No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3060.20504161138584007No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3050.20437154076039615No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2990.20035111700773262No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2980.1996810463822887No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2970.19901097575684476No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2940.19700076388051302No Hit
CAGGAAANAGCTATGANCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2940.19700076388051302No Hit
CAGGAAANAGCTATGACCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC2940.19700076388051302No Hit
CAGGAAANAGCTATGANCCTGGAATTTCAACAGAATGGCATTTATATGAT2930.19633069325506908No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2910.19499055200418125No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT2910.19499055200418125No Hit
CAGGAAANAGCTATGANCCAGGGCTCCAAGAATGTTCCACACATCTTTCT2880.19298034012784948No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2870.19231026950240557No Hit
CAGGAAANAGCTATGANCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2840.1903000576260738No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2820.18895991637518594No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA2770.18560956324796635No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA2750.1842694219970785No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA2710.1815891394953028No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2700.1809190688698589No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG2700.1809190688698589No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2690.18024899824441498No Hit
CAGGAAANAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG2640.17689864511719536No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2610.1748884332408636No Hit
CAGGAAANAGCTATGANNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA2590.17354829198997576No Hit
CAGGAAANAGCTATGANCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2570.1722081507390879No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG2550.17086800948820008No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2550.17086800948820008No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2540.17019793886275614No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2520.1688577976118683No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA2490.16684758573553654No Hit
CAGGAAANAGCTATGANCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2470.16550744448464869No Hit
CAGGAAANAGCTATGANNTGGGTTATGGCGTGGGATGCCCTGCAACCCCC2460.16483737385920477No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2460.16483737385920477No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2360.15813666760476555No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT2350.15746659697932164No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2280.1527761026012142No Hit
CAGGAAANAGCTATGANNTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC2250.15076589072488242No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG2250.15076589072488242No Hit
CAGGAAANAGCTATGANNTGACTTTTGCAAGTCACAGAGAACTGTTGTAT2250.15076589072488242No Hit
CAGGAAANAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA2250.15076589072488242No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2240.15009582009943848No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2200.1474155375976628No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2190.14674546697221888No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT2180.14607539634677497No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2170.14540532572133102No Hit
CAGGAAANAGCTATGANCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2080.13937469009233575No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2070.13870461946689183No Hit
CAGGAAANAGCTATGANNTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA2060.1380345488414479No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG2050.13736447821600398No Hit
CAGGAAACAGCTATGACCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC2040.13669440759056006No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2040.13669440759056006No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2000.13401412508878435No Hit
CAGGAAANAGCTATGACCTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT2000.13401412508878435No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1990.13334405446334044No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1980.13267398383789653No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1880.1259732775834573No Hit
CAGGAAANAGCTATGANCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC1880.1259732775834573No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1870.1253032069580134No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1860.12463313633256945No Hit
CAGGAAANAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT1820.12195285383079377No Hit
CAGGAAANAGCTATGANCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1800.12061271257990593No Hit
CAGGAAANAGCTATGANNAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT1790.119942641954462No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1790.119942641954462No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1780.11927257132901808No Hit
CAGGAAANAGCTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1750.11726235945268632No Hit
CAGGAAANAGCTATGANNGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG1740.11659228882724239No Hit
CAGGAAANAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1740.11659228882724239No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT1740.11659228882724239No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1700.1139120063254667No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1700.1139120063254667No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1690.11324193570002278No Hit
CAGGAAANAGCTATGANCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1670.11190179444913494No Hit
CAGGAAANAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT1670.11190179444913494No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA1670.11190179444913494No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1650.1105616531982471No Hit
CAGGAAANAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1640.10989158257280318No Hit
CAGGAAANAGCTATGANNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1630.10922151194735925No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT1610.10788137069647141No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1600.10721130007102748No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCTGTGA1600.10721130007102748No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1570.10520108819469573No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG1570.10520108819469573No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1570.10520108819469573No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA1560.1045310175692518No Hit
CAGGAAANAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1550.10386094694380787No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1550.10386094694380787No Hit
CAGGAAANAGCTATGANCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1510.10118066444203219No Hit
CAGGAAANAGCTATGANNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC1500.10051059381658828No Hit
CAGGAAANAGCTATGANNGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1500.10051059381658828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)