FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105317
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT43384.118993135011442No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG40183.8151485515159No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC35193.3413409041275393No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC34283.2549351006959935No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT33143.146690467825707No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC26322.4991216992508334No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC25232.3956246379976642No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG22782.16299362875889No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC21082.001576193776883No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG19731.8733917601147014No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC18981.8021781858579338No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC17321.6445588081696212No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC16721.587587948764207No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC15831.5030811739795096No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC15771.497384088038968No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG14701.3957860554326462No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT13891.3188753952353371No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC13461.2780462793281238No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC11911.1308715591974705No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA11691.109982244082152No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC11631.1042851581416107No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT10991.043516241442502No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT10460.993191982301053No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC10370.9846463533902408No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA10100.9590094666578045No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC9020.856461919728059No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT8820.8374716332595877No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT8760.8317745473190464No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA8320.7899959170884093No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT8250.7833493168244443No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG8160.7748036879136322No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT8050.7643590303559729No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA7940.7539143727983136No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG7250.6883978844820875No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA7160.6798522555712753No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG6820.647568768574874No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG6700.6361745966937911No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT6530.6200328531955904No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA6190.5877493661991892No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG5850.5554658792027878No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC5160.48994939088656153No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA5120.4861513335928673No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT4660.442473674715383No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCACTGCAGCACGC4420.4196853309532175No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTTACT4160.39499795854420466No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG4070.38645232963339254No Hit
TGTAAAACGACGGCCAGTTGCTATATGTCGTCCTCTGGAAATGCCATTTA4070.38645232963339254No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGAGAGCGGTTCC3920.372209614782039No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT3860.3665125288414976No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC3780.35891641425410903No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT3740.35511835696041477No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT3530.33517855616851977No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT3350.31808729834689553No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCACTCCAGCCTGG3150.2990970118784242No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG3090.2933999259378828No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT3040.2886523543207649No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGTGACCTGGATGC2970.28200575405679995No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGCTAACTCATAGG2730.2592174102946343No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTGTCCCAGG2720.25826789597121075No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCTTAAGTATGC2540.24117663814958648No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT2530.24022712382616293No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGCTCACT2430.23073198059192723No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGACTGTTCCCACC2330.2212368373576915No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGTTCACT2310.21933780871084438No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC2280.2164892657405737No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA2210.20984266547660874No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGCTGCCCCGGGA2200.20889315115318516No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG2180.20699412250633797No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCATCCTCCTCTTC2110.20034752224237304No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC1990.18895335036129018No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGCCAGTTAACCA1980.18800383603786663No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTAGCTCACT1920.18230675009732522No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGTGTGTGTTCGTGC1920.18230675009732522No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGTTGGGGTCCC1890.17945820712705451No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA1840.17471063550993665No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGACCTTCCAC1810.17186209253966597No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG1770.1680640352459717No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA1770.1680640352459717No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACACCTTC1680.15951840633515957No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTGGCTCACT1680.15951840633515957No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGACTTCTTCTCT1640.15572034904146528No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGCTGAGCGGGTTC1600.151922291747771No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCAGCCCTGTCCCCACA1580.15002326310092387No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTGCTCTGTGTCCC1550.1471747201306532No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTGGCTTACT1450.13767957689641747No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG1410.1338815196027232No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAGGGGCAACC1410.1338815196027232No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACGCCTTC1390.13198249095587605No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCGCTCCCACCCAG1370.13008346230902892No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCACCTG1350.1281844336621818No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGTAGGAGAG1250.11868929042794611No Hit
TGTAAAACGACGGCCAGTAGCACACGTGAATGACCTAAAGATCTTATACG1210.11489123313425183No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGCTGTGGAGATGG1170.11109317584055757No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGGCCCTGCT1170.11109317584055757No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCGGCACCTCT1150.10919414719371041No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGCCTCTTGCTGTTGCTGAT1120.10634560422343971No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCTACAGTGAGACGGGCACC1110.10539608990001614No Hit
TGTAAAACGACGGCCAGTAGGTGGCAAAGCTCAATCAGGACAATTCTGAA1070.10159803260632186No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCAGGCGCCTG1060.1006485182828983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)