FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94208
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT26782.842646059782609No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT23512.495541779891304No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC21002.229110054347826No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG19692.090056046195652No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG17971.9074813179347827No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT16651.767365828804348No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT15291.6230044157608696No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG15231.6166355298913044No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC13821.4669667119565217No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG13451.4276919157608696No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC13311.4128311820652173No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC13111.3916015625No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG13021.382048233695652No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG12661.3438349184782608No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG12611.3385275135869565No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATCACGGTGGT12041.278023097826087No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC11901.2631623641304348No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG11741.2461786684782608No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG11371.2069038722826086No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGGCCCCGGGAGC10901.157014266304348No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG9270.983992866847826No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG9260.9829313858695652No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC8880.9425951086956522No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC8770.9309188179347826No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC8740.927734375No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGTATTTTGCAACTGGGTA7830.8311396059782608No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCTGCAA7320.7770040760869565No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT7110.7547129755434783No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT6620.7027004076086957No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG6590.699515964673913No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG6440.68359375No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCTCACTCGGAT6300.6687330163043478No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC6270.6655485733695652No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG6250.6634256114130435No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC6240.6623641304347826No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCTCCTCCATAGCT6230.6613026494565217No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGCCAGTTAACCA6200.6581182065217391No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG6200.6581182065217391No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC6070.6443189538043478No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT5800.6156589673913043No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTTGGGAAGGGA5780.6135360054347826No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAACCGT5770.6124745244565217No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT5740.6092900815217391No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5580.5923063858695652No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC5350.5678923233695652No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG5180.5498471467391304No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGCTGCCTC5160.5477241847826086No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA4770.5063264266304348No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTCTAGCCTGGGCA4660.4946501358695652No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA4440.4712975543478261No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA4340.4606827445652174No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTGAGG4330.4596212635869565No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG4300.4564368206521739No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC4250.4511294157608696No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT4250.4511294157608696No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT4190.4447605298913043No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCACGGCACTGGT4170.4426375679347826No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCGGTCATAGCCTCT4150.4405146059782609No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC4140.439453125No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCACAGACCCC4100.4352072010869565No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG4040.4288383152173913No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT3880.4118546195652174No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCTCCTCCATGGCT3860.4097316576086957No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG3550.3768257472826087No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC3390.3598420516304348No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC3280.3481657608695652No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT3260.3460427989130435No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTACAGGAACCATGG3250.3449813179347826No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGTACGGCACTGGT3250.3449813179347826No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG3240.34391983695652173No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG3090.3279976222826087No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT3080.32693614130434784No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC2970.31525985054347827No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCAGAGCTG2930.3110139266304348No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG2820.2993376358695652No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCCAGCACGACG2710.2876613451086957No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2660.2823539402173913No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG2630.2791694972826087No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTAGCCTCTCCATT2630.2791694972826087No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC2550.27067764945652173No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT2450.260062839673913No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC2380.252632472826087No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA2310.24520210597826086No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGGTGCCTCAA2240.2377717391304348No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTTAATACATTAGT2240.2377717391304348No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGGAGAGTGA2240.2377717391304348No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGTCAGTGGGAGTCT2230.2367102581521739No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC2110.2239724864130435No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGCTCCACA2060.21866508152173914No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCTAGCACGACG2060.21866508152173914No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG1970.2091117527173913No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCTGAGCGAGATAA1940.2059273097826087No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC1890.20061990489130435No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGGGCCGGTCCGT1840.1953125No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGGGCCGGCCCGT1770.18788213315217392No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTGTAAAACGACG1750.18575917119565216No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTAGAGATGGGGT1720.18257472826086957No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGAACCATGGCT1680.17832880434782608No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT1650.1751443614130435No Hit
TGTAAAACGACGGCCAGTGGCTCTATATCAGGGCCCGCCGGTCATAGCTG1630.17302139945652176No Hit
TGTAAAACGACGGCCAGTTTTCCCCTTCTCCCTCCAGGACCCCAAAGGCT1610.1708984375No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGTCCAACAATGGCCTG1530.16240658967391305No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGGATGGAGGGG1510.1602836277173913No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGTAGGAGAG1510.1602836277173913No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGCTGGGGCCGGA1490.15816066576086957No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG1480.1570991847826087No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA1440.15285326086956522No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCGGTCTAGCCTCTC1400.14860733695652176No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCCCGTAAATGATA1360.14436141304347824No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCACTAGGAGAG1360.14436141304347824No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGCCGAGACCCAGCGC1310.13905400815217392No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGTGCACGGGAGC1290.13693104619565216No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACTGCCTTCCAGCATTT1280.1358695652173913No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC1220.12950067934782608No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCACACAGACCCC1210.12843919836956522No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTGCACTCCAGCTG1170.12419327445652174No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGATAGGGCCAGGC1150.1220703125No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGCATCCTGGGTGAC1140.12100883152173912No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGGAGGGCTACAGTC1120.1188858695652174No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCGAGGGAGGATCC1100.11676290760869565No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG1100.11676290760869565No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1090.11570142663043477No Hit
TGTAAAACGACGGCCAGTTTTCCCCTTCTCCCTCCAGGCCCCCAAAGGCT1040.11039402173913043No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAAGGAGAGTG1020.1082710597826087No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGTGGGGACGGA990.10508661684782608No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTGGCCCTTC980.10402513586956523No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGGGGCCGGGAGG970.10296365489130435No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG960.10190217391304347No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)