FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85960
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA8881.0330386226151698No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC8771.0202419730107026No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA6290.7317356910190786No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC6040.7026523964634714No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC5940.6910190786412285No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC5770.6712424383434156No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT5550.6456491391344812No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT4810.5595625872498837No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4600.5351326198231736No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4330.5037226617031177No Hit
CAGGAAANAGCTATGANNACCCCACCTGCTCAAAAGACACACTCCTAAAA4190.4874360167519777No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT4090.47580269892973476No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT4090.47580269892973476No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4060.4723127035830619No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC4020.4676593764541648No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT3900.4536993950674732No Hit
CAGGAAANAGCTATGANNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT3780.43973941368078173No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3780.43973941368078173No Hit
CAGGAAANAGCTATGANCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3750.43624941833410885No Hit
CAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3730.43392275476966025No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3590.41763610981852023No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3510.4083294555607259No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3420.3978594695207073No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG3260.37924616100511865No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3190.3711028385295486No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3130.36412284783620286No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2790.324569567240577No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT2770.32224290367612846No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2770.32224290367612846No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC2730.3175895765472313No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT2720.31642624476500697No Hit
CAGGAAANAGCTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA2650.308282922289437No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2620.30479292694276405No Hit
CAGGAAANAGCTATGACCACCCCACCTGCTCAAAAGACACACTCCTAAAA2590.3013029315960912No Hit
CAGGAAANAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2500.2908329455560726No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT2490.2896696137738483No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2460.28617961842717543No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2450.28501628664495116No Hit
CAGGAAANAGCTATGACCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT2440.2838529548627268No Hit
CAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2430.28268962308050255No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC2390.2780362959516054No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2310.2687296416938111No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2300.2675663099115868No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2300.2675663099115868No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2290.2664029781293625No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2270.26407631456491387No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA2270.26407631456491387No Hit
CAGGAAANAGCTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC2270.26407631456491387No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2260.2629129827826896No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT2250.2617496510004653No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2250.2617496510004653No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2220.25825965565379244No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2200.2559329920893439No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2170.252442996742671No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2160.25127966496044674No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2120.24662633783154955No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2050.23848301535597952No Hit
CAGGAAANAGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG2040.2373196835737552No Hit
CAGGAAACAGCTATGACCACCCCACCTGCTCAAAAGACACACTCCTAAAA1990.23150302466263378No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1980.23033969288040948No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1980.23033969288040948No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1950.2268496975337366No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1900.22103303862261517No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1890.21986970684039087No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT1850.21521637971149374No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1770.2059097254536994No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1680.19543973941368079No Hit
CAGGAAANAGCTATGANCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1630.18962308050255933No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1620.18845974872033502No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1610.18729641693811075No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1610.18729641693811075No Hit
CAGGAAANAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1580.18380642159143787No Hit
CAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1570.18264308980921357No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1560.18147975802698932No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAATT1560.18147975802698932No Hit
CAGGAAACAGCTATGACCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT1540.1791530944625407No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC1540.1791530944625407No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA1540.1791530944625407No Hit
CAGGAAANAGCTATGANCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1540.1791530944625407No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1530.1779897626803164No Hit
CAGGAAANAGCTATGANCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1520.17682643089809214No Hit
CAGGAAANAGCTATGANNGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG1510.17566309911586786No Hit
CAGGAAANAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1510.17566309911586786No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT1500.17449976733364356No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1500.17449976733364356No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG1480.17217310376919498No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1480.17217310376919498No Hit
CAGGAAANAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1470.17100977198697068No Hit
CAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1470.17100977198697068No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1470.17100977198697068No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1470.17100977198697068No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1460.1698464402047464No Hit
CAGGAAANAGCTATGANNGGGTCCCACCCATGCTGAAAGTCCACCTTCCA1450.1686831084225221No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1440.16751977664029782No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1420.16519311307584922No Hit
CAGGAAANAGCTATGANCACCCCACCTGCTCAAAAGACACACTCCTAAAA1400.16286644951140067No Hit
CAGGAAANAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC1380.16053978594695206No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1360.15821312238250348No Hit
CAGGAAANAGCTATGANCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1360.15821312238250348No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1350.1570497906002792No Hit
CAGGAAANAGCTATGANNCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT1330.1547231270358306No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1330.1547231270358306No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1310.15239646347138203No Hit
CAGGAAANAGCTATGANCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1310.15239646347138203No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAACT1310.15239646347138203No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1300.15123313168915775No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1290.15006979990693345No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1270.14774313634248487No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT1260.1465798045602606No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1260.1465798045602606No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1260.1465798045602606No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1250.1454164727780363No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1230.14308980921358772No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1220.1419264774313634No Hit
CAGGAAANAGCTATGANCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT1220.1419264774313634No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1190.13843648208469056No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG1180.13727315030246626No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1180.13727315030246626No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1180.13727315030246626No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGTGACACA1180.13727315030246626No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1160.13494648673801768No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1150.1337831549557934No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCAG1130.1314564913913448No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1120.13029315960912052No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1120.13029315960912052No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1110.12912982782689622No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1100.12796649604467195No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1080.12563983248022337No Hit
CAGGAAANAGCTATGANCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1070.12447650069799907No Hit
CAGGAAANAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1070.12447650069799907No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1060.12331316891577478No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1030.11982317356910191No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1020.1186598417868776No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1020.1186598417868776No Hit
CAGGAAACAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1010.11749651000465333No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1010.11749651000465333No Hit
CAGGAAANAGCTATGANNGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG1000.11633317822242904No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1000.11633317822242904No Hit
CAGGAAANAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC980.11400651465798045No Hit
CAGGAAANAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC970.11284318287575618No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC970.11284318287575618No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC970.11284318287575618No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA960.11167985109353187No Hit
CAGGAAACAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC960.11167985109353187No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT960.11167985109353187No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC960.11167985109353187No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC950.11051651931130758No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC940.1093531875290833No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG930.10818985574685902No Hit
CAGGAAANAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA930.10818985574685902No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG930.10818985574685902No Hit
CAGGAAANAGCTATGANCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA920.10702652396463472No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT920.10702652396463472No Hit
CAGGAAANAGCTATGANNGGGAGGTGGAGGTTGCAGTGAGTTGAGATCTC910.10586319218241043No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG900.10469986040018613No Hit
CAGGAAANAGCTATGANCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT900.10469986040018613No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC890.10353652861796185No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG880.10237319683573756No Hit
CAGGAAANAGCTATGANCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC880.10237319683573756No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA870.10120986505351326No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA870.10120986505351326No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG860.10004653327128897No Hit
CAGGAAANAGCTATGANCCCGTAGCCACGCCCCTATTGTCTGACCACGCT860.10004653327128897No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)