FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103008
Sequences flagged as poor quality0
Sequence length300
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC19521.8949984467225849No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT17031.6532696489593042No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAAGGAAAATGGAGA14971.4532851817334576No Hit
CAGGAAACAGCTATGACCGGGCATGGTGTGGGACTAGCTAATCTAGGGAC14851.4416356011183598No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCAGGGCTGCAGAAC14081.3668841255048152No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC12941.2562131096613856No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC12361.1999068033550793No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT11501.1164181422802113No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC11481.1144765455110281No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT11451.1115641503572538No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT11401.1067101584342964No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCGGGTGACCACG10901.0581702392047219No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG10871.0552578440509475No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCAGGAAACAGCT10060.976623174899037No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC9920.9630319975147562No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA9850.9562364088226157No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC9690.940703634669152No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG9530.9251708605156881No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC9400.9125504815159988No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT8920.8659521590556073No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA8680.8426529978254117No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG8520.8271202236719477No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC8510.8261494252873564No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC8350.8106166511338926No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT8340.809645852749301No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT8190.7950838769804286No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG8180.7941130785958372No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT7940.7708139173656415No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC7690.7465439577508542No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC7490.7271279900590245No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA7370.7154784094439267No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGTCGTTTTACATG7270.7057704255980118No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT6820.6620844982913948No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCCCAAGACACTG6700.650434917676297No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC6640.6446101273687481No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT6210.602865796831314No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC6210.602865796831314No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6100.5921870146008077No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA5710.5543258776017397No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT5700.5533550792171482No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG5680.5514134824479653No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACCCAAGACA5580.5417054986020503No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA5560.5397639018328674No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC5480.5319975147561354No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC5390.523260329294812No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA5390.523260329294812No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCCACAGTGTCATT5380.5222895309102206No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC5350.5193771357564462No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5230.5077275551413483No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG5090.4941363777570674No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGTCCTCCCTTGCT5070.4921947809878844No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCGGACGAGGA5040.48928238583411No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4990.4844283939111525No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG4990.4844283939111525No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT4760.4621000310655483No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCACGTGTGCTGTGG4700.4562752407579994No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4690.4553044423734079No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4680.4543336439888164No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC4590.44559645852749297No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG4530.43977166821994407No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTGAGCTGTTGAGA4460.43297607952780365No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT4450.4320052811432122No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA4160.40385212799005904No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC4090.3970565392979186No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC4080.39608574091332716No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG4010.3892901522211867No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT3970.38540695868282077No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACAGGGGCGATAGTG3960.38443616029822925No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA3870.37569897483690584No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAACACACACACAC3820.37084498291394846No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT3740.3630785958372165No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT3480.33783783783783783No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGACTATGCTGAA3420.33201304753028893No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGAAGAGGAGCC3420.33201304753028893No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG3410.3310422491456974No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3390.3291006523765144No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC3350.3252174588381485No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT3310.32133426529978254No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG3090.2999767008387698No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG3080.29900590245417835No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTCAGGAAACAGCT3060.2970643056849953No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCGTGTGCGCT2970.2883271202236719No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT2970.2883271202236719No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2940.28541472506989746No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTTGAGCTCCTCA2930.284443926685306No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTCTAAGGGGACAG2920.2834731283007145No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCACAGACCCCACA2840.27570674122398264No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG2830.2747359428393911No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG2740.2659987573780677No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT2690.2611447654551103No Hit
CAGGAAACAGCTATGACCGGGCCTTCTCGAAGCTCTCCCAGGAAGCGCCC2690.2611447654551103No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG2660.25823237030133583No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2610.2533783783783784No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2550.24755358807082944No Hit
CAGGAAACAGCTATGACCCCAGCAGAGCTCTTCTTACAGAGGCAGGAAAC2520.244641192917055No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC2500.24269959614787198No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCAGCGGG2490.24172879776328052No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAGATTTTCAAT2390.23202081391736565No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGCATCCTGG2360.22910841876359117No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATTCTCCTCACACC2350.2281376203789997No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2340.2271668219944082No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCGGAGAGCCTGTG2300.22328362845604222No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG2250.2184296365330848No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCCGTGTGCGCTG2200.21357564461012735No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2160.2096924510717614No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCGTGTGCGCTGG2150.20872165268716994No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA2130.20678005591798693No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA2120.20580925753339546No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2120.20580925753339546No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCAGGACACCCCA2110.204838459148804No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGTCCTGTGCCCA2110.204838459148804No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA2100.20386766076421248No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCCACAGAGAGCAC2000.1941596769182976No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGGGCGGA1990.19318887853370612No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1930.1873640882261572No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC1890.18348089468779122No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCACTGTACTCAGA1880.18251009630319975No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1870.18153929791860826No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGTTTCTGTTCCAT1800.17474370922646784No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1790.17377291084187638No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGAAGGGCCACCCCA1780.17280211245728488No Hit
CAGGAAACAGCTATGACCGGGCCTTCTCGAAGCTCTCCAGGAAGCGCCCC1770.17183131407269336No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC1740.16891891891891891No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGAGGAGCCGGT1710.16600652376514446No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA1690.16406492699596148No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGAAGCTCCAGCA1650.1601817334575955No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC1640.15921093507300405No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAACCAGTGAC1630.15824013668841255No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGCCCAGCCTTCA1610.15629853991922957No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCTCCAGAACAC1560.15144454799627213No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGACCCTGGCAG1550.15047374961168064No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1550.15047374961168064No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTGGGGGAATGGGA1530.14853215284249766No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1470.14270736253494873No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGGTCAACCTGCT1450.14076576576576577No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCCGATTC1440.13979496738117428No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1430.1388241689965828No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1430.1388241689965828No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGTCACTGCAGCCTGGG1420.13785337061199132No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCGGGCAGAGG1410.1368825722273998No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGACAGAAGCCCA1390.13494097545821684No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTCCTCAGCCACC1370.13299937868903386No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG1360.13202858030444237No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC1310.12717458838148493No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCAGCCACCACCACGTACCACT1290.12523299161230197No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTCTGGGAGGTGGG1280.12426219322771047No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC1280.12426219322771047No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGAAGAACAGCGAG1260.1223205964585275No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCGGGCAGAG1240.12037899968934453No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGTGCCACTGCACTGCA1240.12037899968934453No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTGCCGTGTGCGC1240.12037899968934453No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAACCAACGGTGC1190.11552500776638708No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCTGAGAGCCTGTG1180.11455420938179559No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATACCTGCTCTGGG1170.1135834109972041No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGACCGGCCCCTCA1160.11261261261261261No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGACGCTGCCCTGC1140.11067101584342964No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCATTGG1140.11067101584342964No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATCCTCAGATTCC1110.10775862068965517No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGGCACAGCAACTC1110.10775862068965517No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTTGATGGCCTCAG1100.10678782230506367No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGGCTCCACCTGCC1100.10678782230506367No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCTGA1100.10678782230506367No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGATGCCACG1090.1058170239204722No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG1080.1048462255358807No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCAAAATTATCCT1060.10290462876669773No Hit
CAGGAAACAGCTATGACCACCACACCTGGCCTGAGGATGCTGTCCTGGGT1040.10096303199751476No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC1040.10096303199751476No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG1040.10096303199751476No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)