FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82433
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT15171.8402824111702836No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG9081.1015006126187328No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT8961.0869433357999831No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT8591.0420583989421712No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA8571.0396321861390463No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT8100.9826161852656097No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT7630.9256001843921731No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT7550.9158953331796732No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7280.8831414603374862No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA6960.8443220554874867No Hit
CAGGAAANAGCTATGANNTGGCCCAGCCTATGCCTTTCTAATTGCTCTAG6200.7521259689687383No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG5420.6575036696468647No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG5190.6296022224109277No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG4620.5604551575218663No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG4390.5325537102859291No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA4190.5082915822546796No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT4110.49858673104217976No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT4060.4925211990343673No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4020.4876687734281173No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT3810.4621935389953053No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG3730.4524886877828055No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3710.45006247497968044No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG3660.4439969429718681No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3660.4439969429718681No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG3480.42216102774374337No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3250.39425958050780635No Hit
CAGGAAANAGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC3170.3845547292953065No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3170.3845547292953065No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3150.3821285164921815No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT3110.3772760908859316No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2970.3602926012640568No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2950.3578663884609319No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2840.3445222180437446No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2830.34330911164218214No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT2790.3384566860359322No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2740.3323911540281198No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2710.32875183482343234No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2650.32147319641405747No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2570.31176834520155766No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2510.30448970679218273No Hit
CAGGAAANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2390.289932429973433No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA2330.28265379156405807No Hit
CAGGAAANNGCTATGANNTGGCCCAGCCTATGCCTTTCTAATTGCTCTAG2190.26567030194218333No Hit
CAGGAAANNGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2140.2596047699343709No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2040.24747370591874612No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC2040.24747370591874612No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2010.24383438671405866No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1940.23534264190312135No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG1930.23412953550155882No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG1880.22806400349374645No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1850.22442468428905898No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1770.21471983307655915No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1750.21229362027343415No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1740.21108051387187168No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1730.20986740747030921No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1690.2050149818640593No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT1640.19894944985624688No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1620.19652323705312194No Hit
CAGGAAANAGCTATGANNCGGGACCCAAAGTTCTGCGTGATGGTCAGCAC1570.1904577050453095No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1540.18681838584062208No Hit
CAGGAAANAGCTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC1530.1856052794390596No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1520.1843921730374971No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCAG1480.17953974743124718No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1470.1783266410296847No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT1460.17711353462812224No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1450.17590042822655977No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1420.1722611090218723No Hit
CAGGAAANNGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC1410.1710480026203098No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1360.16498247061249743No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1360.16498247061249743No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1340.16255625780937247No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1310.158916938604685No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG1300.15770383220312253No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1290.15649072580156007No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG1270.1540645129984351No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1270.1540645129984351No Hit
CAGGAAANAGCTATGANNGGTCCCGCGGGGAAAGTAAGTCCTTCCCTTGG1220.1479989809906227No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1150.1395072361796853No Hit
CAGGAAANNGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1130.13708102337656036No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1120.1358679169749979No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1110.1346548105734354No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1100.13344170417187293No Hit
CAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1100.13344170417187293No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1100.13344170417187293No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1070.12980238496718546No Hit
CAGGAAANAGCTATGANNTGACTGCAAACATAGAAGTGAGCAGAGCAAGA1060.128589278565623No Hit
CAGGAAANNGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1030.12494995936093556No Hit
CAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1030.12494995936093556No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1020.12373685295937306No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG1000.1213106401562481No Hit
CAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT990.12009753375468563No Hit
NAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT950.1152451081484357No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT950.1152451081484357No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC950.1152451081484357No Hit
NAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT940.11403200174687322No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA930.11281889534531073No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA930.11281889534531073No Hit
CAGGAAANNGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT930.11281889534531073No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA910.11039268254218577No Hit
CAGGAAANNGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA900.1091795761406233No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTATTCCCAACCA900.1091795761406233No Hit
CAGGAAANNGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT900.1091795761406233No Hit
NAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG890.10796646973906081No Hit
CAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA870.10554025693593584No Hit
NAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG870.10554025693593584No Hit
CAGGAAANAGCTATGANNAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT870.10554025693593584No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT870.10554025693593584No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG870.10554025693593584No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA870.10554025693593584No Hit
CAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT860.10432715053437337No Hit
NAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA850.10311404413281089No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT850.10311404413281089No Hit
NAGGAAANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT840.10190093773124842No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA830.10068783132968592No Hit
CAGGANANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT830.10068783132968592No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)