FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150573
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT35302.3443778100987562No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT32922.186314943582183No Hit
TGTAAAANGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG16211.0765542295099386No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG15501.0294010214314653No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG14150.9397435131132408No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT13800.916498973919627No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG13410.8905979159610288No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG13080.8686816361499073No Hit
TGTAAAANGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA12620.8381316703525864No Hit
TGTAAAANGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC12520.8314903734401253No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG12360.8208642983801877No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT11800.7836730356704057No Hit
TGTAAAANGACGGCCAGTGAACAGCCCCGGAAGCAAGTAGGGAGGAGAGG11550.7670697933892531No Hit
TGTAAAANGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG11500.7637491449330226No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG11480.7624208855505303No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG11400.7571078480205614No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA11360.754451329255577No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG11340.7531230698730849No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC11230.7458176432693776No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG11060.7345274385181938No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAGGGAGGAGAGG10720.7119470290158262No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC10510.698000305499658No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGTCAT10420.692023138278443No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG10230.6794046741447669No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGTCAT10160.6747557663060443No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC9710.6448699301999695No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA9450.6276025582275707No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC9430.6262742988450785No Hit
TGTAAAANGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA9310.6183047425501252No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC9010.5983808518127419No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA8980.5963884627390037No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC8890.5904112955177887No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC8890.5904112955177887No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC8570.5691591453979133No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC8430.5598613297204678No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA8390.5572048109554834No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG7940.5273189748494086No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGAGGTGG7900.5246624560844242No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCTGCCAA7840.5206776779369475No Hit
TGTAAAANGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCTGCCAA7760.5153646404069787No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGAGGTGG7510.49876139812582604No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG7210.47883750738844283No Hit
TGTAAAANGACGGCCAGTTGGCCCACTGTGCTAGCCTACAGGAACCATGG7170.47618098862345837No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTACAGGAACCATGG6910.45891361665105956No Hit
TGTAAAANGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA6900.4582494869598135No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG6700.4449668931348914No Hit
TGTAAAANGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC6620.4396538556049225No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA6530.43367668838370754No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA6520.43301255869246147No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA6390.4243788727062621No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC6390.4243788727062621No Hit
TGTAAAACGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA6310.4190658351762932No Hit
TGTAAAANGACGGCCAGTAAAATCAGGCCGGGCGTGGGTGACAGAGTGTG6100.40511911166012493No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG6080.4037908522776328No Hit
TGTAAAANGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC5800.3851952209227418No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGGTGACAGAGTGTG5800.3851952209227418No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA5710.37921805370152684No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCAGGTGTA5650.37523327555405017No Hit
TGTAAAANGACGGCCAGTTCACCATGTTGGCCAGGCTGTGTAAAACGACG5510.3659354598766047No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG5360.3559735145079131No Hit
TGTAAAANGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT5290.35132460666919035No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC5190.3446833097567293No Hit
TGTAAAANGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC5140.3413626613004988No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTGTAAAACGACG5140.3413626613004988No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG5130.3406985316092527No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG5120.34003440191800655No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG5060.33604962377052994No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT5010.3327289753142994No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5010.3327289753142994No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCAGGTGTA4890.32475941901934613No Hit
TGTAAAANGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT4880.3240952893281No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC4860.3227670299456078No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT4720.31346921426816227No Hit
TGTAAAANGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC4660.30948443612068566No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT4660.30948443612068566No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC4450.29553771260451744No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCTGCTGTCCTCA4380.29088880476579465No Hit
TGTAAAANGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA4360.28956054538330245No Hit
TGTAAAANGACGGCCAGTACCTGAATGCTCGCAGCTCCCTGCTGTCCTCA4310.2862398969270719No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG4270.2835833781620875No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT4110.2729573031021498No Hit
TGTAAAANGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATGCCCCTGCC4080.2709649140284115No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT4060.26963665464591924No Hit
TGTAAAANGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC4040.26830839526342704No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGCCAG4040.26830839526342704No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT3810.25303341236476656No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC3810.25303341236476656No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG3740.24838450452604383No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGCCAG3730.24772037483479772No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC3640.24174320761358278No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTCTTGGCTCACT3630.24107907792233668No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC3600.23908668884859838No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC3560.23643017008361392No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTTGGCTCACT3550.23576604039236781No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT3530.2344377810098756No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATGCCCCTGCC3500.23244539193613728No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA3470.23045300286239898No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG3420.2271323544061684No Hit
TGTAAAANGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT3390.2251399653324301No Hit
TGTAAAANGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC3360.22314757625869178No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG3350.2224834465674457No Hit
TGTAAAANGACGGCCAGTCTCTCACCCACCTCCACCCACTGGAGGTCATA3300.21916279811121514No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC3260.21650627934623073No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT3260.21650627934623073No Hit
TGTAAAANGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG3250.2158421496549846No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA3220.21384976058124633No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG3190.21185737150750797No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3150.20920085274252356No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG3150.20920085274252356No Hit
TGTAAAANGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG3130.20787259336003133No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC3120.20720846366878523No Hit
TGTAAAACGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT3110.2065443339775391No Hit
TGTAAAANGACGGCCAGTCCTCACTCCCCACCCAGGATAGCTGTTTCCTG3100.20588020428629306No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCACTGGAGGTCATA3090.20521607459504693No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3090.20521607459504693No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT3060.2032236855213086No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC3010.19990303706507806No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT3000.19923890737383196No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC2950.19591825891760142No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG2930.19458999953510922No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA2880.19126935107887869No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2870.19060522138763258No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG2850.18927696200514035No Hit
TGTAAAANGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG2760.18329979478392538No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTTCCTGGGGCAGA2730.18130740571018708No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATAGCTGTTTCCTG2720.18064327601894098No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT2670.17732262756271044No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT2670.17732262756271044No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTTCCTGGGGCAGA2640.1753302384889721No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT2640.1753302384889721No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG2600.1726737197239877No Hit
TGTAAAANGACGGCCAGTGAACAGCCCCGGAAGCAAGTAGGGAGGTCATA2540.16868894157651107No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAGGGAGGTCATA2520.16736068219401884No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGAGGAACCATGG2450.1627117743552961No Hit
TGTAAAANGACGGCCAGTTGGCCCACTGTGCTAGCCTAGAGGAACCATGG2430.1613835149728039No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT2410.1600552555903117No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG2400.15939112589906557No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2400.15939112589906557No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT2360.15673460713408113No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGAGGCAC2160.14345201330915902No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCATAGCTGTTTCC2130.1414596242354207No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT1990.13216180855797519No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG1980.1314976788667291No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT1970.13083354917548298No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC1950.12950528979299078No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGAACCATGGCT1910.12684877102800635No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT1910.12684877102800635No Hit
TGTAAAANGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGAACCATGGCT1890.12552051164551414No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCATAGCTGTTTCC1820.12087160380679139No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGAGGCAC1810.12020747411554528No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGTGTA1800.11954334442429919No Hit
TGTAAAANGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC1790.11887921473305307No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC1780.11821508504180696No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC1760.11688682565931476No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT1710.1135661772030842No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGTGTA1700.11290204751183812No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC1660.11024552874685369No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT1630.10825313967311537No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG1560.10360423183439262No Hit
TGTAAAANGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC1520.1009477130694082No Hit
TGTAAAANGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACAGCCCCTGC1510.1002835833781621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)