FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150573
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA24381.6191481872580078No Hit
CAGGAAANAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA13720.9111859363896582No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT12300.8168795202327112No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTCAGGAAACA9910.6581525240248915No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC9120.6056862784164492No Hit
CAGGAAANAGCTATGANCTGGAAACCCATGCCATGCACTCAACACTACAA9110.6050221487252031No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT8960.5950602033565114No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA8850.5877547767528043No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA8680.5764645720016205No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8470.5625178484854523No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8280.5498993843517762No Hit
CAGGAAANAGCTATGANNTCCCTGCTTGATAATGACCCCTCTCCTCCCTA7830.5200135482457015No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA7450.4947766199783494No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC7380.49012771213962664No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTCAGGAAACA6560.43566907745744593No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6460.42902778054498486No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCAGGAAACAGCT6240.41441692733757046No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5900.39183651783520285No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5740.38121044277526517No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC5740.38121044277526517No Hit
CAGGAAANAGCTATGANNAGCAAAGGTCTTGGCAGGGGTGGGGACTGGAA5650.37523327555405017No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC5400.3586300332728975No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA5350.355309384816667No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5290.35132460666919035No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5270.34999634728669815No Hit
CAGGAAANAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA5130.3406985316092527No Hit
CAGGAAANAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT4910.3260876784018383No Hit
CAGGAAANAGCTATGANNACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG4780.31745399241563893No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4660.30948443612068566No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG4660.30948443612068566No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG4590.30483552828196286No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4460.2962018422957635No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA4390.2915529344570408No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC4380.29088880476579465No Hit
CAGGAAANAGCTATGACCTCCCTGCTTGATAATGACCCCTCTCCTCCCTA4290.2849116375445797No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT4230.28092685939710305No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTCAGGAAACA4230.28092685939710305No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4200.27893447032336477No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4180.2776062109408725No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT4140.2749496921758881No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCACGCCCGGCCT4080.2709649140284115No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC4060.26963665464591924No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAGTGTTCC4030.26764426557218096No Hit
CAGGAAANAGCTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC4020.26698013588093483No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT3950.2623312280422121No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT3850.255689931129751No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT3760.24971276390853606No Hit
CAGGAAANAGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT3710.24639211545230555No Hit
CAGGAAANAGCTATGANNTCCCTGCTTGATAATGACCCCTCTCAGGAAAC3700.24572798576105942No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTCAGGAAACA3650.24240733730482888No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT3470.23045300286239898No Hit
CAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3450.22912474347990675No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3400.22580409502367624No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3400.22580409502367624No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG3380.224475835641184No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3360.22314757625869178No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3320.22049105749370737No Hit
CAGGAAANAGCTATGACCAGCAAAGGTCTTGGCAGGGGTGGGGACTGGAA3310.21982692780246124No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3270.21717040903747684No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG3250.2158421496549846No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3170.2105291121250158No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC3150.20920085274252356No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA3150.20920085274252356No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3100.20588020428629306No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG3050.20255955583006247No Hit
CAGGAAANAGCTATGANCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3020.2005671667563242No Hit
CAGGAAANAGCTATGANCCTGGAATTTCAACAGAATGGCATTTATATGAT3000.19923890737383196No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG3000.19923890737383196No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2980.19791064799133976No Hit
CAGGAAANAGCTATGACCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC2960.19658238860884752No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2930.19458999953510922No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGAGCTGCGAGCAT2930.19458999953510922No Hit
CAGGAAACAGCTATGACCTCCCTGCTTGATAATGACCCCTCTCCTCCCTA2910.193261740152617No Hit
CAGGAAANAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG2880.19126935107887869No Hit
CAGGAAANAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT2870.19060522138763258No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2840.18861283231389425No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA2810.18662044324015595No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2810.18662044324015595No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA2760.18329979478392538No Hit
CAGGAAANAGCTATGANCTCCCTGCTTGATAATGACCCCTCTCCTCCCTA2740.18197153540143318No Hit
CAGGAAANAGCTATGANNCATGTGCCCCACTGATGCTCTCAATGTGTACT2720.18064327601894098No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTGGCCCCAAT2710.17997914632769488No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2680.17798675725395655No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2640.1753302384889721No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2630.17466610879772604No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2630.17466610879772604No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2610.1733378494152338No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT2570.1706813306502494No Hit
CAGGAAANAGCTATGANNGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC2550.16935307126775717No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA2540.16868894157651107No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2510.16669655250277274No Hit
CAGGAAANAGCTATGANNAGCAAAGGTCTTGGCAGGGGCATGGGGACTGG2500.16603242281152664No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2490.16536829312028054No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACACAGGAAACAGCT2490.16536829312028054No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2440.16204764466405No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2430.1613835149728039No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT2420.16071938528155777No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2410.1600552555903117No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2400.15939112589906557No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2390.15872699620781947No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2330.15474221806034283No Hit
CAGGAAANAGCTATGACCTCCCTGCTTGATAATGACCCCTCTCAGGAAAC2290.1520856992953584No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2210.14677266176538953No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT2200.14610853207414345No Hit
CAGGAAANAGCTATGACCGTCAACAGCATACCTGTACACCTGAGTGTTCC2190.14544440238289733No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTGGGGACTGGAA2180.14478027269165122No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG2160.14345201330915902No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCACGCCCGGCCT2160.14345201330915902No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT2150.1427878836179129No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGTCGTCATCCACC2150.1427878836179129No Hit
CAGGAAANAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT2100.13946723516168238No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2100.13946723516168238No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2090.13880310547043626No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2080.13813897577919015No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2080.13813897577919015No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2070.13747484608794405No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2060.13681071639669795No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC2050.13614658670545182No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG2040.13548245701420575No Hit
CAGGAAANAGCTATGANCGCTGCTGACCCAACCCTACCCAGGAAACAGCT2020.13415419763171352No Hit
CAGGAAANAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG2020.13415419763171352No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2010.13349006794046742No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT2000.13282593824922131No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2000.13282593824922131No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1960.13016941948423688No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC1950.12950528979299078No Hit
CAGGAAACAGCTATGANCTGGAAACCCATGCCATGCACTCAACACTACAA1930.12817703041049855No Hit
CAGGAAANAGCTATGANNGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG1930.12817703041049855No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1920.12751290071925248No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1890.12552051164551414No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAGGAAACAGCTA1890.12552051164551414No Hit
CAGGAAANAGCTATGANNTGGGTTATGGCGTGGGATGCCAGGAAACAGCT1890.12552051164551414No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1870.12419225226302191No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1860.12352812257177583No Hit
CAGGAAANAGCTATGANCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1850.12286399288052971No Hit
CAGGAAANAGCTATGANNACCCAGTTAGAGCCATGGTTCCTCTAGGCTAG1810.12020747411554528No Hit
CAGGAAANAGCTATGANCAGCAAAGGTCTTGGCAGGGGTGGGGACTGGAA1800.11954334442429919No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1790.11887921473305307No Hit
CAGGAAANAGCTATGANNCCAATAAATAAATACAGAACCTTGGAACTGGC1770.11755095535056087No Hit
CAGGAAANAGCTATGANNGATGCTCCTTCCTTGGCCCCGGTACTGGCCGT1740.11555856627682252No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1720.11423030689433032No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCACGCCCGGCCT1720.11423030689433032No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT1720.11423030689433032No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGAGCTGCGAGCAT1710.1135661772030842No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1710.1135661772030842No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCCAGGAAAGGCAG1690.112237917820592No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT1660.11024552874685369No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1660.11024552874685369No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1650.10958139905560757No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1650.10958139905560757No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1640.10891726936436148No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1620.10758900998186925No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1620.10758900998186925No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1610.10692488029062316No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCACGCCCGGCCT1610.10692488029062316No Hit
CAGGAAANAGCTATGANCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC1610.10692488029062316No Hit
CAGGAAANAGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1590.10559662090813093No Hit
CAGGAAANAGCTATGACCTCCAGTGGTGGGGCTCAACACAGGAAACAGCT1560.10360423183439262No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1520.1009477130694082No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)