FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122427
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT51034.168198191575388No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT44943.6707589012227695No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC44223.6119483447278786No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT36682.9960711281008274No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT35792.923374745766865No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC31672.586847672490545No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA31252.5525415145351924No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT31062.5370220621268187No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG28762.3491550066570284No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC25922.11718003381607No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC24371.9905739746951245No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG24241.979955401994658No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA22871.8680519819974353No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT18571.5168222695973927No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG18461.5078373234662288No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG17891.461278966241107No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG16901.380414451060632No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT16501.3477419196745817No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT14201.1598748642047914No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA12941.0569563903387325No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG12701.037352871507102No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCCAGGAAACAGCT12291.0038635268364005No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG12240.9997794604131441No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA12180.9948785807052366No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA11770.9613892360345347No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT11320.9246326382252281No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT10870.8878760404159213No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC9740.7955761392503288No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG9480.774338993849396No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG8890.7261470100549715No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT8440.6893904122456648No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG8150.6657028269907782No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG7680.6273126026121688No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC7290.5954568845107697No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA7100.5799374321023957No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC6960.5685020461172781No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA6330.5170428091842485No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA6120.49988973020657207No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC6080.496622477067967No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGT6030.4925384106447107No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGACAGGAAACA5950.4860039043675006No Hit
CAGGAAACAGCTATGACCTGACTGCAAACATAGAAGTGAGCAGAGCAAGA5890.48110302465959304No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA5290.43209422758051735No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA5100.4165747751721434No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA4340.3544969655386475No Hit
CAGGAAACAGCTATGACCTCCCTGCTTGATAATGACCCCTCTGAATTCTT4130.33734388656097103No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTGTTC3670.299770475467013No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC3520.28751827619724407No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG3320.2711820105042188No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG3240.2646475042270087No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTTTCAGGGAAGC2920.2385094791181684No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGCAGAGGG2850.23279178612560955No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2620.21400508057863055No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTCTTC2530.2066537610167692No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGGTCCCGGGA2520.20583694773211791No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCTGTGA2440.1993024414549078No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCCCCTGCCTACC2030.16581309678420608No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAAATCATTACCTT1990.16254584364560104No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCTGAGATCGC1960.16009540379164727No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGTGGGGCCAGGTTTACTG1790.14620957795257583No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1780.14539276466792456No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG1780.14539276466792456No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGGAAACAGCTA1760.14375913809862204No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC1730.14130869824466824No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCCCTTCCTTACCT1650.13477419196745816No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTA1630.13314056539815564No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCTGAGATCAT1530.12497243255164302No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1510.12333880598234048No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCATCTCGCTGAGA1470.12007155284373544No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCACAGGGACG1470.12007155284373544No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTTTCACATCTTG1420.11598748642047915No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCTCCATACAGAGG1390.11353704656652537No Hit
CAGGAAACAGCTATGACCTGGAGCCAATTTCATGAGGGCAGACAGCACGT1270.10373528715071022No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGGGATGAGGT1240.10128484729675644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)