FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177935
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT47142.6492820411948186No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT32211.8102115941214487No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC29861.678140894146739No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT24161.3577991963357405No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC22741.277994773372299No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC21341.1993143563660886No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT20481.150982100205131No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT20151.1324360019108102No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT17831.0020513108719475No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG15830.8896507151487903No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA14970.8413184589878325No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT14950.840194453030601No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT13130.7379099109225279No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA12320.6923876696546492No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC12090.6794616011464861No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA11950.6715935594458651No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG11750.6603534998735493No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC10970.6165172675415179No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCCAGGAAACAGCT10480.5889791215893444No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC10380.5833590918031866No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT10310.579425070952876No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG9570.5378368505353078No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT8840.4968106330963554No Hit
CAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC7930.4456683620423188No Hit
CAGGAAANNGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC7310.41082417736814003No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT7030.39508809396689804No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA7010.3939640880096664No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA6620.37204597184365074No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT6380.3585579003568719No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGCTATGACCC6270.35237586759209827No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT5900.33158175738331414No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5880.3304577514260826No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC5860.32933374546885097No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC5810.32652373057577205No Hit
CAGGAAANNGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA5710.32090370078961417No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG5460.30685362632421953No Hit
CAGGAAANNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT5420.30460561440975636No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG5150.2894315339871301No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG4980.27987748335066176No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4900.2753814595217355No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4890.2748194565431197No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4800.2697614297355776No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4780.268637423778346No Hit
CAGGAAANAGCTATGANNTCCAGCTGCTCCATCTTGTCTACCTCCCGGCC4700.26414139994941976No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4660.2618933880349566No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC4530.25458734931295135No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA4450.2500913254840251No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4370.2455953016550988No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC4370.2455953016550988No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4120.23154522718970408No Hit
CAGGAAANNGCTATGANNCAGAAGGCTGAGCAGGAGGCCAGGAAACAGCT4050.22761120633939358No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3890.21861915868154103No Hit
CAGGAAANNGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3840.21580914378846208No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3830.2152471408098463No Hit
CAGGAAANNGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC3820.2146851378312305No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3750.21075111698092No Hit
CAGGAAANNGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC3730.20962711102368842No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG3630.20400708123753056No Hit
CAGGAAANAGCTATGANNCTGGTCCTCATCCTTGGGGCTTGAGCTGAGAG3540.19894905442998848No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTACGTA3530.19838705145137267No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC3530.19838705145137267No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA3500.19670104251552534No Hit
CAGGAAANAGCTATGANNAACACCCTTGGCCTCCCTCACTGGCCGTCGTT3460.1944530306010622No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG3410.19164301570798326No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT3290.1848989799645938No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3230.1815269620928991No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3220.1809649591142833No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3200.17984095315705173No Hit
CAGGAAANAGCTATGANNAGGTCCAGATGGCTGGTGACTTTAGGCCTTAG3130.17590693230674123No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3120.17534492932812543No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3100.17422092337089387No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2990.1680388906061202No Hit
NAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2880.16185685784134657No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2850.16017084890549918No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2800.15736083401242026No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2740.15398881614072554No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2650.1489307893331835No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCCTGGGCAACAG2630.14780678337595188No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2620.1472447803973361No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGCTATGACCC2580.14499676848287296No Hit
CAGGAAACAGCNATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2570.14443476550425718No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2530.14218675358979402No Hit
CAGGAANNNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2530.14218675358979402No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2530.14218675358979402No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2490.13993874167533088No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2440.13712872678225194No Hit
NAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2410.1354427178464046No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG2390.134318711889173No Hit
NAGGANANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2350.13207069997470988No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACGCAGCTAGTAAA2300.12926068508163094No Hit
NAGGANANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2290.12869868210301513No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2270.12757467614578358No Hit
CAGGANANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2270.12757467614578358No Hit
NAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2260.12701267316716777No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2220.12476466125270465No Hit
NAGGAAANNGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2210.12420265827408886No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCCCTTCCTTACCT2150.12083064040239413No Hit
CAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2150.12083064040239413No Hit
NAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2140.12026863742377834No Hit
NAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2100.11802062550931519No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGTGGGGCCAGGTTTACTG2100.11802062550931519No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2060.11577261359485204No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2040.11464860763762048No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCAGGAAACAGCTA1970.11071458678730997No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1960.11015258380869418No Hit
CAGGANANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1920.10790457189423104No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT1900.10678056593699946No Hit
NAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1890.1062185629583837No Hit
CAGGANANNGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1890.1062185629583837No Hit
CAGGAAANAGCTATGANNAGACAGGGTCTTGCTATGTTGCCCAGGTAGTG1880.10565655997976789No Hit
CAGGAAANNGCTATGANNTCCAGCTGCTCCATCTTGTCTACCTCCCGGCC1870.1050945570011521No Hit
NAGGANANNGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1850.10397055104392054No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1850.10397055104392054No Hit
NAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1840.10340854806530475No Hit
CAGGAAANNGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC1830.10284654508668895No Hit
NAGGANANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1830.10284654508668895No Hit
CAGGAAACNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1790.10059853317222582No Hit
CAGGANANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1790.10059853317222582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)