FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164408
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT49573.0150600943992996No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT48262.935380273466011No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA45682.7784536032309863No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA44792.7243199844289814No Hit
TGTAAAANGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG27551.6757092112305971No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGATGCCTGGGTGGG27501.6726679966911586No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGATGCCTGGGTGGG26641.620359106612817No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG25341.5412875285874166No Hit
TGTAAAANGACGGCCAGTGTTCTACCGTCAGTCCTGGCATAGCTGTTTCC18631.1331565373947738No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG18171.10517736363194No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATAGCTGTTTCC17411.0589509026324753No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG17411.0589509026324753No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC16230.9871782395017275No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC15710.9555496082915673No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG13920.84667412777967No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG13470.8193031969247238No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG13280.8077465816748577No Hit
TGTAAAANGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC13210.8034888813196438No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC12710.773076735925259No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC12490.7596953919517299No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGAGGTGG11910.7244173032942436No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC11910.7244173032942436No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG11830.7195513600311421No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC11760.7152936596759282No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC10950.666025984137025No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGAGGTGG10920.6642012554133618No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC10810.6575105834265973No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC10330.6283149238479879No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT10280.6252737093085494No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG10250.6234489805848864No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC10130.616150065690234No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT9360.5693153617828817No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC9290.5650576614276678No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG8970.5455938883752616No Hit
TGTAAAANGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC8900.5413361880200477No Hit
TGTAAAANGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG8730.5309960585859569No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC8630.5249136295070799No Hit
TGTAAAANGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA8600.5230889007834169No Hit
TGTAAAACGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG7830.4762541968760644No Hit
TGTAAAACGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA7600.46226460999464747No Hit
TGTAAAANGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA7360.44766678020534284No Hit
TGTAAAANGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC7200.4379348936791397No Hit
TGTAAAANGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT7180.43671840786336436No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT7180.43671840786336436No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC7050.42881125006082427No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG6850.41664639190307046No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC6730.4093474770084181No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT6700.407522748284755No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA6530.3971826188506642No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG6500.3953578901270011No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT6420.3904919468638996No Hit
TGTAAAANGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC6360.3868424894165734No Hit
TGTAAAANGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC6250.38015181742980875No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG6250.38015181742980875No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC6190.3765023599824826No Hit
TGTAAAANGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT6000.36494574473261643No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCATAGCTGTTTCC5960.3625127731010656No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC5790.3521726436669748No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC5600.34061602841710864No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC5530.3363583280618948No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG5460.3321006277066809No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCATAGCTGTTTCC5210.3168945550094886No Hit
TGTAAAACGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT5040.3065544255753978No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC5030.3059461826675101No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT4980.3029049681280716No Hit
TGTAAAANGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT4790.29134835287820543No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC4790.29134835287820543No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCACCGTG4690.28526592379932847No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC4660.2834411950756654No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCACCGTG4550.2767505230889008No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4380.26641039365481No Hit
TGTAAAANGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC4330.2633691791153715No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG4320.26276093620748386No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG4250.25850323585227No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG4170.2536372925891684No Hit
TGTAAAANGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA4150.252420806773393No Hit
TGTAAAACGACGGCCAGTGCCTGTGCCTGACCACTACACTGGTCATAGCT4020.24451364897085298No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA4000.2432971631550776No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC3890.23660649116831298No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC3840.23356527662887452No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC3800.23113230499732373No Hit
TGTAAAANGACGGCCAGTGCCTGTGCCTGACCACTACACTGGTCATAGCT3710.22565811882633446No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA3700.2250498759184468No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG3670.2232251471947837No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG3660.222616904286896No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG3450.20984380322125445No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC3390.20619434577392828No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC3380.20558610286604057No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA3040.18490584399785898No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2920.17760692910320666No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC2910.17699868619531897No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCAGGTGCTGG2810.170916257116442No Hit
TGTAAAANGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC2760.16787504257700356No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA2760.16787504257700356No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2740.16665855676122815No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA2740.16665855676122815No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA2740.16665855676122815No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCAGGTGCTGG2680.163009099313902No Hit
TGTAAAANGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG2610.15875139895868814No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG2600.15814315605080045No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT2580.15692667023502505No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA2570.15631842732713735No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC2560.15571018441924966No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA2520.1532772127876989No Hit
TGTAAAANGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC2510.15266896987981118No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGATAGGGCCAGGC2480.1508442411561481No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC2470.15023599824826042No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGGGGACCTGCTGGAG2440.14841126952459735No Hit
TGTAAAANGACGGCCAGTGTTCTACCGTCAGTCCTGGCAGGCTGTGCTTA2440.14841126952459735No Hit
TGTAAAANGACGGCCAGTCACCTTCCCTCTGCTGCTGGATAGGGCCAGGC2390.14537005498515887No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG2360.1435453262614958No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTGTCCCAGG2300.13989586881416963No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCAGGCTGTGCTTA2290.13928762590628194No Hit
TGTAAAACGACGGCCAGTGCCTGTGCCTGACCACTACACACTGGTCATAG2270.13807114009050653No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT2270.13807114009050653No Hit
TGTAAAANGACGGCCAGTGCCTGTGCCTGACCACTACACACTGGTCATAG2210.13442168264318038No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC2180.1325969539195173No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT2150.1307722251958542No Hit
TGTAAAANGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT2130.12955573938007883No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2130.12955573938007883No Hit
TGTAAAANGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG2130.12955573938007883No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA2120.12894749647219114No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC2110.12833925356430345No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2080.12651452484064035No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGGGGACCTGCTGGAG2060.12529803902486497No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2040.12408155320908958No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2030.12347331030120189No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGGGAGGTGGA1970.11982385285387571No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG1970.11982385285387571No Hit
TGTAAAANGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA1950.11860736703810032No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT1950.11860736703810032No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT1890.11495790959077418No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTGTCCCAGG1850.1125249379592234No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1840.11191669505133571No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT1790.10887548051189723No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG1780.10826723760400954No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG1780.10826723760400954No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA1750.10644250888034645No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGTTGGGATTGGGAGAGGGA1750.10644250888034645No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA1730.10522602306457106No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC1700.10340129434090797No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1670.10157656561724492No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGGGAGGTGGA1670.10157656561724492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)