FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164408
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC28791.751131331808671No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT26871.634348693494234No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC19591.1915478565519926No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGGCATCCTCCTCG18111.101527906184614No Hit
CAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT16481.0023843121989198No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT14970.9105396331078779No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCCAGGAAAC12910.7852415940830131No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC12860.7822003795435746No Hit
CAGGAAANAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC11650.7086029876891635No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA11490.6988711011629605No Hit
CAGGAAANAGCTATGACCGGTAGCAACGTCCCACCCAGGCATCCTCCTCG11060.6727166561237896No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT10910.6635930125054742No Hit
CAGGAAANAGCTATGANCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT10200.6204077660454479No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC9870.6003357500851539No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC9640.5863461632037371No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA9330.5674906330592185No Hit
CAGGAAANAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT9200.5595834752566785No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA8950.5443774025594862No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA8630.5249136295070799No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8620.5243053865991922No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC8110.49328499829691985No Hit
CAGGAAANAGCTATGACCAGAGACCAGATGCATAAGCACAGCCAGGAAAC7570.4604398812709844No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG7330.4458420514816797No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG7180.43671840786336436No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCATCCTCCTCG7170.4361101649554766No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT7120.43306895041603816No Hit
CAGGAAANAGCTATGANNAACACCCTTGGCCTCCCTCACTGGCCGTCGTT6860.4172546348109581No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA6800.4136051773636319No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT6570.399615590482215No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC6430.3911001897717873No Hit
CAGGAAANAGCTATGANCGGTAGCAACGTCCCACCCAGGCATCCTCCTCG6310.38380127487713495No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC6220.3783270887061457No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC5940.3612962872852903No Hit
CAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA5750.34973967203542405No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5520.3357500851540071No Hit
CAGGAAANAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA5430.33027589898301785No Hit
CAGGAAANAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA5390.3278429273514671No Hit
CAGGAAANAGCTATGACCAACACCCTTGGCCTCCCTCACTGGCCGTCGTT5270.32054401245681474No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5140.3126368546542747No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT5100.31020388302272395No Hit
CAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5060.3077709113911732No Hit
CAGGAAANAGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC5050.3071626684832855No Hit
CAGGAAANAGCTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC4920.29925551068074546No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4910.29864726777285777No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4830.2937813245097562No Hit
CAGGAAANAGCTATGANCAGAGACCAGATGCATAAGCACAGCCAGGAAAC4810.2925648386939808No Hit
CAGGAAACAGCTATGACCAGAGACCAGATGCATAAGCACAGCCAGGAAAC4780.29074010997031774No Hit
CAGGAAANAGCTATGANNTGACTTTTGCAAGTCACAGAGAACTGTTGTAT4490.2731010656415746No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA4480.2724928227336869No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT4470.2718845798257992No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4360.2651939078390346No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4350.2645856649311469No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT4320.26276093620748386No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG4100.24937959223395456No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4000.2432971631550776No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA3980.24208067733930222No Hit
CAGGAAANAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT3950.24025594861563915No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3920.23843121989197605No Hit
CAGGAAANAGCTATGANNTGACTGCAAACATAGAAGTGAGCAGAGCAAGA3900.23721473407620064No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA3900.23721473407620064No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC3860.2347817624446499No Hit
CAGGAAANAGCTATGANNAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT3830.2329570337209868No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA3820.23234879081309914No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3750.22809109045788525No Hit
CAGGAAANAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC3680.22383339010267142No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3540.21531798939224367No Hit
CAGGAAANAGCTATGANCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA3540.21531798939224367No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3500.2128850177606929No Hit
CAGGAAANAGCTATGANCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3430.20862731740547905No Hit
CAGGAAANAGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT3420.20801907449759133No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3420.20801907449759133No Hit
CAGGAAACAGCTATGACCAACACCCTTGGCCTCCCTCACTGGCCGTCGTT3190.1940294876161744No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3160.19220475889251132No Hit
CAGGAAANAGCTATGANCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3150.19159651598462363No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3090.18794705853729746No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA3040.18490584399785898No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGGAAACA3030.18429760108997129No Hit
CAGGAAANAGCTATGACCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC3020.1836893581820836No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGTGAGCTCCTCAG2980.1812563865505328No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2970.18064814364264514No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2950.17943165782686976No Hit
CAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2930.17821517201109435No Hit
CAGGAAANAGCTATGANCAACACCCTTGGCCTCCCTCACTGGCCGTCGTT2920.17760692910320666No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2910.17699868619531897No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2910.17699868619531897No Hit
CAGGAAANAGCTATGANNAGTGTGAAGTCTGGATCAAATATTGCCCAGGA2900.17639044328743128No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2790.16969977130066663No Hit
CAGGAAANAGCTATGACCACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC2780.16909152839277894No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT2760.16787504257700356No Hit
CAGGAAACAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2740.16665855676122815No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2710.1648338280375651No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT2630.15996788477446353No Hit
CAGGAAANAGCTATGANCCAGGGCTCCAAGAATGTTCCACACATCTTTCT2560.15571018441924966No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG2560.15571018441924966No Hit
CAGGAAANAGCTATGACCTGACTTTTGCAAGTCACAGAGAACTGTTGTAT2550.15510194151136197No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2550.15510194151136197No Hit
CAGGAAANAGCTATGANNGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG2540.15449369860347428No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2440.14841126952459735No Hit
CAGGAAANAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT2430.14780302661670966No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2370.1441535691693835No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2370.1441535691693835No Hit
CAGGAAANAGCTATGACCTGACTGCAAACATAGAAGTGAGCAGAGCAAGA2330.1417205975378327No Hit
CAGGAAANAGCTATGANCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2280.13867938299839425No Hit
CAGGAAANAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC2280.13867938299839425No Hit
CAGGAAANAGCTATGANCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2270.13807114009050653No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2240.13624641136684346No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2240.13624641136684346No Hit
CAGGAAANAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT2180.1325969539195173No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2180.1325969539195173No Hit
CAGGAAANAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2160.13138046810374193No Hit
CAGGAAACAGCTATGANCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2120.12894749647219114No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG2070.12590628193275266No Hit
CAGGAAANAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA2030.12347331030120189No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA1980.1204320957617634No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGAAACAGCTA1930.11739088122232495No Hit
CAGGAAANAGCTATGANCACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC1920.11678263831443726No Hit
CAGGAAANAGNTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1900.11556615249866187No Hit
CAGGAAANAGCTATGACCCTTGAGCTCCTCGGACACGGTGAGCTCCTCAG1900.11556615249866187No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1890.11495790959077418No Hit
CAGGAAANAGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1880.11434966668288649No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1860.11313318086711109No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1850.1125249379592234No Hit
CAGGAAACAGCTATGACCACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC1850.1125249379592234No Hit
CAGGAAANAGCTATGANCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1850.1125249379592234No Hit
CAGGAAANAGCTATGANNAGTGTGAAGTCTGGATCAAATATTGCCAGGAA1840.11191669505133571No Hit
CAGGAAANAGCTATGANCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC1830.111308452143448No Hit
NAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1830.111308452143448No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1820.11070020923556032No Hit
CAGGAAACAGCTATGACCTGACTTTTGCAAGTCACAGAGAACTGTTGTAT1810.11009196632767262No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1810.11009196632767262No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCCAGGAAACAGCT1810.11009196632767262No Hit
CAGGAAANAGCTATGACCAGTGTGAAGTCTGGATCAAATATTGCCCAGGA1800.10948372341978492No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCACCTGGCTCCT1790.10887548051189723No Hit
NAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1770.10765899469612183No Hit
CAGGAAANAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGGAAACA1760.10705075178823414No Hit
CAGGAAANAGCTATGACCTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT1750.10644250888034645No Hit
NAGGAAANAGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1730.10522602306457106No Hit
NAGGAAANAGNTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1730.10522602306457106No Hit
CAGGAAACAGCTATGACCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC1720.10461778015668337No Hit
CAGGAAANAGCTATGACCGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG1680.1021848085251326No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1670.10157656561724492No Hit
CAGGAAACAGCTATGACCTGACTGCAAACATAGAAGTGAGCAGAGCAAGA1660.10096832270935721No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)