FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172180
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT29701.724939017307469No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT27191.5791613427808109No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG26481.5379254268788478No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT25841.5007550238122895No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC25771.4966895109768845No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT23891.3875014519688698No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG23761.3799512138459753No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT23611.3712394006272506No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT23411.359623649668951No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC22091.2829596933441747No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA21961.27540945522128No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT21761.2637937042629805No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA20411.1853873852944592No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT20091.1668021837611802No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT19831.1517017075153908No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT18911.0982692531072134No Hit
CAGGAAACAGCTATGACCTCTACGCCCCTCAGCTGTGTCCTGTCTGCATA16570.9623649668951098No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC16550.9612033917992799No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA16300.9466837031014055No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC16250.9437797653618306No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG16190.9402950400743407No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT15930.9251945638285516No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC15750.914740387966082No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC14790.8589847833662446No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA14380.8351724939017308No Hit
CAGGAAACAGCTATGACCTGCACACACCTCCTATGTTAACAGGCACAAGT14240.8270414682309211No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT13790.8009060285747475No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT13580.788709490068533No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG13260.7701242885352538No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT13060.7585085375769544No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG13030.7567661749332094No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG12770.7416656986874202No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC12220.7097223835520966No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT12160.7062376582646068No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA11840.6876524567313277No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG11630.6754559182251133No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA11430.6638401672668137No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA11360.659774654431409No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG10960.6365431525148101No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA9830.5709141596004181No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAAGGAAAATGGAGA9800.5691717969566732No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG9390.5453595074921593No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG9010.5232895806713904No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG8840.5134161923568359No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG8700.5052851666860263No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT8480.49250784063189684No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG8430.48960390289232203No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA8250.4791497270298524No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA8230.4779881519340225No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA8140.47276106400278783No Hit
CAGGAAACAGCTATGACCAGCAACATACCGTTTGTAAACAACTGGCCGTC8100.47043791381112793No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA8070.468695551167383No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT8000.46463003833197813No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC7930.46056452549657334No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG7800.4530142873736787No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG7720.4483679869903589No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA7670.44546404925078403No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT7610.4419793239632942No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT7580.4402369613195493No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT7240.4204901846904402No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC7070.4106167963758857No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC7050.40945522128005574No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA6950.403647345800906No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACAGGGGCGATAGTG6930.4024857707050761No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA6890.40016262051341617No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT6810.3955163201300964No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC6680.3879660820072018No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG6380.37054245556975257No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC6300.3658961551864328No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG6210.36066906725519804No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC6100.35428040422813334No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT6060.3519572540364735No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCGTGTGCGCT5910.3432454408177488No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG5810.33743756533859914No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG5740.33337205250319435No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC5720.33221047740736437No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT5650.3281449645719596No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA5630.32698338947612965No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGACGTTGCCCTGC5620.3264026019282147No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG5520.32059472644906495No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA5480.31827157625740504No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC5430.3153676385178302No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT5180.30084794981995583No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT5150.29910558717621094No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG5090.2956208618887211No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC4940.2869090486699965No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG4870.2828435358345917No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT4780.27761644790335693No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCAGGGCTGCAGAAC4720.2741317226158671No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC4720.2741317226158671No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT4710.27355093506795214No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC4660.2706469973283773No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT4520.2625159716575677No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCTGA4480.2601928214659078No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC4390.25496573353467306No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4370.2538041584388431No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT4240.24625392031594842No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCACAGGAAGCAGAA4110.2387036821930538No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC4080.23696131954930888No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGCCCACACGGGC4060.23579974445347893No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA4060.23579974445347893No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG4030.234057381809734No Hit
CAGGAAACAGCTATGACCTCTACGCCCCTCAGCTGTGTGGAGTCATTCTC3920.22766871878266928No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCCACAGAGAGCAC3910.22708793123475432No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG3900.22650714368683936No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG3870.22476478104309444No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG3690.21431060518062495No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGAGGAGCCGGT3680.21372981763270993No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC3620.2102450923452201No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCGGGCAGAGG3490.20269485422232547No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACACCTCCCCTGG3480.2021140666744105No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA3450.2003717040306656No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACAGAGGAGGCGAGC3430.19921012893483564No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTATGTGACT3340.1939830410036009No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG3290.19107910326402602No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTTGGAGTCCCAC3150.1829480775932164No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT3110.1806249274015565No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG3100.18004413985364154No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC3060.17772098966198163No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCGCAGTGGGGCCA3020.17539783947032175No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTTCGTGGGGAA3010.1748170519224068No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA3000.17423626437449183No Hit
CAGGAAACAGCTATGACCAGAGCTACTTCTGTGCCCACCATATTCCTCTA2990.17365547682657684No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC2990.17365547682657684No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACACACACTTCTAA2950.17133232663491693No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCTCCTTCAG2880.16726681379951214No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCACACCCACCC2700.15681263793704264No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG2700.15681263793704264No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCAAGACACTGGCAGGT2660.15448948774538274No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC2640.15332791264955278No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCCGTGTGCGCTG2570.149262399814148No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT2560.148681612266233No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG2540.14752003717040307No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTCTGGGAAGTGAG2500.1451968869787432No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC2490.1446160994308282No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGGAGGGGTGGAGC2480.14403531188291321No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC2480.14403531188291321No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA2450.1422929492391683No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2440.14171216169125334No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGTCACTGCAGCCTGGG2430.14113137414333837No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG2410.13996979904750842No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTGTGGCCGA2360.13706586130793355No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG2300.13358113602044372No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG2300.13358113602044372No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2280.13241956092461377No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC2260.13125798582878384No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2260.13125798582878384No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTGCCGTGTGCGC2250.13067719828086885No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCCACTGGTCCGT2240.1300964107329539No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCGGGCAG2190.12719247299337902No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGTCAAGA2140.12428853525380415No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG2070.12022302241839936No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTAACAAACTCCC2070.12022302241839936No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC2040.11848065977465444No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1970.11441514693924962No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGGCTTGTAAC1880.10918805900801487No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTTCCTGCAACAC1870.10860727146009991No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG1860.10802648391218493No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCCACAGTGTCATT1820.10570333372052503No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGGTACCCGTGTC1810.10512254617261005No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCTCAGGTGATCT1760.1022186084330352No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1750.10163782088512023No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1740.10105703333720525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)