FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142219
Sequences flagged as poor quality0
Sequence length300
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT56523.9741525393934705No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG41492.9173317207967995No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAGCCGAAGCTGT32162.2613012326060513No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC31782.2345818772456565No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT30722.160048938608765No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT29162.050358953445039No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT24621.7311329709813739No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG24041.6903507970102447No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG23501.6523811867612628No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC23121.6256618314008677No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC22371.5729262616106146No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGGTCAGTGTTCCT21911.5405817788059262No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT21661.5230032555425084No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGCAGCTGCCATGT18661.312060976381496No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG18531.302920144284519No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT18341.2895604666043214No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA16971.1932301591207926No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA16071.1299474753724887No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG15251.072289919068479No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG14371.0104135171812487No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG14261.0026789669453449No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG13490.9485371152940184No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG12570.8838481496846413No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG12080.8493942440883426No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG12080.8493942440883426No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCAGGGCAGAAGGG11810.8304094389638516No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG11760.826893734311168No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT11690.821971747797411No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC11630.8177529022141907No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT11530.8107214929088237No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG11440.8043932245339934No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGGCCCCGGGAGC11320.7959555333675529No Hit
TGTAAAACGACGGCCAGTTGCTGCATATTATTCCATTATGCAGACAGGAC11140.7832989966178922No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC11130.7825958556873553No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCACTTCCTGC10880.7650173324239378No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA9820.6904843937870467No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAAGCAAC9780.6876718300649No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCTGGGAGAGT9550.6714995886625557No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACGGCAGGAGA9230.648999078885381No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA9190.6461865151632342No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC9190.6461865151632342No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG9040.6356394012051836No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA8420.5920446635119078No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA8340.586419536067614No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC7810.5491530667491685No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGTGCCATGTTTCC7760.5456373620964851No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGTT7670.5393090937216546No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGCCAGTACCGGG7340.5161054430139432No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT7170.5041520471948192No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGGCCCTGCT6750.47462012811227755No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGATGGCACTGA6690.47040128252905733No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT6500.45704160484885986No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG5920.41625943087773076No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC5870.41274372622504724No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCACGGCACTGGT5590.39305578017001946No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCCACTGGAGTGCA5550.39024321644787263No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGCATCCTGGGTGAC5550.39024321644787263No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT5540.38954007551733594No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAGCCTCC5460.3839149480730423No Hit
TGTAAAACGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG5380.3782898206287486No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG5340.3754772569066018No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTGCTGCCTCT5280.37125841132338155No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCTGGGCAGAAGGG5070.3564924517821107No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT4870.34242963317137654No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC4860.34172649224083984No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCACAGACCCC4690.3297730964217158No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCTGCTGTCCTCA4680.32906995549117907No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT4570.3213354052552753No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCTGTGGGAGACCCAGC4450.3128977140888348No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGGTGACAGAGTGTG4370.30727258664454116No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGTCAGTGGGAGTCT4100.2882877815200501No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG3810.26789669453448556No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA3780.2657872717428754No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCAGAGCTG3680.25875586243750837No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCTCCCCCTGCTG3540.24891188940999445No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGGTGCCTCAA3450.2425836210351641No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACACTCCCC3390.23836477545194382No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCTGTGGGAGA3340.2348490707992603No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG3320.2334427889381869No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCCTAAGGGAG3200.22500509777174638No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCCAGCACGACG3200.22500509777174638No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGTACGGCACTGGT3190.22430195684120965No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCAGTGCCTTGCCAG3090.21727054753584263No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT2960.20812971543886538No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCCGGAGTGCAATG2940.206723433577792No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCGCTCCCACCCAG2820.1982857424113515No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC2740.19266061496705783No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACCGCCGAGTG2690.1891449103143743No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG2520.17719151449525028No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC2500.1757852326341769No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG2470.17367580984256675No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGCAGATCCCAGAG2410.1694569642593465No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTTTCCTGTCA2340.16453497774558953No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTACGGCCAGTACC2300.16172241402344273No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA2170.15258158192646554No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTAGCACAGGGTTG2170.15258158192646554No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC2130.14976901820431868No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCATCCTCCTCTTC2100.14765959541270857No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC2080.14625331355163515No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCTAGG2070.14555017262109846No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC2040.14344074982948832No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG2020.1420344679684149No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTGTTTCCTGACA2000.14062818610734149No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT1960.13781562238519468No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT1920.13500305866304782No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTTCACTCTGTGT1860.1307842130798276No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCACATGTACTCT1820.12797164935768077No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCTAGCACGACG1820.12797164935768077No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCCTGCTGTCCTC1800.12656536749660735No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGGGAGGGAGGAG1790.12586222656607063No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCCAGGGGGCGTCCCGG1790.12586222656607063No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTGTAAAACGACGG1780.1251590856355339No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG1740.1223465219133871No Hit
TGTAAAACGACGGCCAGTCGCAGAAGCAAGGGGCTGAATGGTTTGGTGGG1660.11672139446909345No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGGAGGGCTACAGTC1640.11531511260802003No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCTGGGCAGAAGG1620.1139088307469466No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACATCCTGGGCTGGGGC1610.11320568981640991No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGGCAGGAGAG1580.11109626702479977No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGACCCACGAGGGCT1530.10758056237211625No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGAATGG1520.10687742144157954No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCCACTCCGTTGCC1500.10547113958050613No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCTTG1470.103361716788896No Hit
TGTAAAACGACGGCCAGTGGCTCTATATCAGGGCCCGCCGGTCATAGCTG1460.10265857585835929No Hit
TGTAAAACGACGGCCAGTCGCAGAAGCAAGGGGCTGAAAAGATCTGAAGG1460.10265857585835929No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCATCTTGGGTGCCACAGG1450.1019554349278226No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACAGCCTGGGTGG1450.1019554349278226No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)