FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113862
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT5670.4979712283290299No Hit
NNGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT4750.4171716639440727No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4700.4127803832709771No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT4550.3996065412516906No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCTGCCCAACACCC4430.38906746763626143No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT4030.35393722225149743No Hit
NNGGANNNNGNTATGNNNAGGTTGCTGGATTCAGTTTGCTGACCAACTTT3960.34778942930916373No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT3760.3302243066167817No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT3700.3249547698090671No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT3700.3249547698090671No Hit
NNGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT3580.3144156961936379No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3550.3117809277897806No Hit
NNGGANNNNGNTATGNNNTGAGTTAACGTGATTTAGGACCCTGGCTGTTT3480.3056331348474469No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA3460.3038766225782087No Hit
NNGGANNNNGNTATGNNNGATGGCAGAATGGAAACATGGCAGCTGCCTTG3420.3003635980397323No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT3380.29685057350125593No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCTGCCCAACACCC3340.2933375489627795No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC3320.2915810366935413No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT3080.2705028894626829No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT3020.2652333526549683No Hit
NNGGANNNNGNTATGNNNAAAACCAAGTCACCTGTTGATCTGTCAGGAAA2850.2503029983664436No Hit
NNGGANNNNGNTATGANNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC2850.2503029983664436No Hit
NNGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT2820.24766822996258628No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2770.2432769492894908No Hit
NNGGANNNNGNTATGNNNGCATCACTGAGGGAGGATCCTGTGGGGGAGCC2740.2406421808856335No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2630.2309813634048234No Hit
NNGGANNNNGNTATGNNNAAAACCAAGTCACCTGTTGATCTGTGCAGGAA2610.2292248511355852No Hit
NNGGANNNNGNTATGANNGATGGCAGAATGGAAACATGGCAGCTGCCTTG2560.22483357046248967No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGTCGTTTTACATG2550.22395531432787058No Hit
NAGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT2540.22307705819325147No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT2520.22132054592401326No Hit
NNGGANNNNGNTATGANNAGGTTGCTGGATTCAGTTTGCTGACCAACTTT2520.22132054592401326No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2490.21868577752015594No Hit
NNGGANNNNGNTATGANNTGAGTTAACGTGATTTAGGACCCTGGCTGTTT2470.2169292652509178No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCTGCCCAACACCC2460.21605100911629868No Hit
NNGGANNNNGNTATGNNNGCCCCTACTCCAAAAGGCGGCAGAGAGTGGAG2350.20639019163548855No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2330.2046336793662504No Hit
NNGGANNNNGNTATGNNNGGCCTTCTCTGCATTATCTCGCTAATCTCGAG2270.19936414255853577No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2240.19672937415467848No Hit
NNGGANNNNGNTATGANNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT2110.18531204440463017No Hit
NNGGANNNNGNTATGNNNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA2110.18531204440463017No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2110.18531204440463017No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2100.18443378827001106No Hit
NNGGANNNNGNTATGNNNAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT2060.18092076373153468No Hit
NNGGANNNNGNTATGANNGCATCACTGAGGGAGGATCCTGTGGGGGAGCC2040.17916425146229648No Hit
NNGGANNNNGNTATGANNAAAACCAAGTCACCTGTTGATCTGTGCAGGAA1990.17477297078920098No Hit
NNGGANNNNGNTATGANNGCCCCTACTCCAAAAGGCGGCAGAGAGTGGAG1970.17301645851996278No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1950.17125994625072455No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1920.16862517784686726No Hit
NNGGANNNNGNTATGANNCTTGGTGGAGCCATAGGCCGTCGTTTTACATG1920.16862517784686726No Hit
NNGGANNNNGNTATGNNNGCCCCTACTCCAAAAGGCGGTAGAGAGTGGAG1910.16774692171224817No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1910.16774692171224817No Hit
NNGGANNNNGNTATGNNNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT1870.16423389717377176No Hit
NNGGANNNNGNTATGANNAAAACCAAGTCACCTGTTGATCTGTCAGGAAA1870.16423389717377176No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1860.16335564103915265No Hit
NNGGANNNNGNTATGNNNTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC1850.16247738490453356No Hit
NAGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1800.15808610423143804No Hit
NNGGANNNNGNTATGNNNACAGCTTTCTGAGGGTTGTTCCAGGCGCGGTG1760.15457307969296166No Hit
NNGGANNNNGNTATGNNNGCATCCTGCTTAGTGCTGCCCACAGTGTCATT1760.15457307969296166No Hit
NNGGANNNNGNTATGANNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA1740.15281656742372346No Hit
NAGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1740.15281656742372346No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1730.15193831128910434No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1720.15106005515448526No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1710.15018179901986614No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1700.14930354288524705No Hit
NNGGANNNNGNTATGANNAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT1690.14842528675062797No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1690.14842528675062797No Hit
NNGGANNNNGNTATGANNTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC1650.14491226221215156No Hit
NAGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1630.14315574994291336No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1630.14315574994291336No Hit
NNGGANNNNGNTATGNNNACAGCTTTCTGAGGGTTGTTCCAGGTGCGGTG1630.14315574994291336No Hit
NAGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCTGCCCAACACCC1620.14227749380829424No Hit
NNGGANNNNGNTATGNNNACCGTTAAGCCACACCAAATGGTGAGCCCTGC1610.14139923767367515No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGGTACCCGTGTC1600.14052098153905607No Hit
NAGGANNNNGNTATGNNNGATGGCAGAATGGAAACATGGCAGCTGCCTTG1590.13964272540443695No Hit
NAGGANNNNGNTATGNNNTGAGTTAACGTGATTTAGGACCCTGGCTGTTT1570.13788621313519875No Hit
NAGGANNNNGNTATGNNNAGGTTGCTGGATTCAGTTTGCTGACCAACTTT1570.13788621313519875No Hit
NAGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1550.13612970086596055No Hit
NNGGANNNNGNTATGANNGCCCCTACTCCAAAAGGCGGTAGAGAGTGGAG1550.13612970086596055No Hit
NNGGANNNNGNTATGNNNGCTTCTATATGAGCGCATCTCACCTGAATTTG1550.13612970086596055No Hit
NNGGANNNNGNTATGNNNACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT1550.13612970086596055No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1550.13612970086596055No Hit
NNGGANNNNGNTATGNNNAACCAACACTGCTGCTAATTGTGTTTGTGTCC1530.13437318859672234No Hit
NAGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1510.13261667632748414No Hit
NNGGANNNNGNTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG1510.13261667632748414No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1500.13173842019286505No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1460.12822539565438865No Hit
NAGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT1440.12646888338515044No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGGTACCCGTGTC1420.12471237111591224No Hit
NNGGANNNNGNTATGANNGGCCTTCTCTGCATTATCTCGCTAATCTCGAG1420.12471237111591224No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1420.12471237111591224No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1410.12383411498129314No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1400.12295585884667404No Hit
NAGGANNNNGNTATGNNNAAAACCAAGTCACCTGTTGATCTGTCAGGAAA1390.12207760271205495No Hit
NNGGANNNNGNTATGNNNACCCCACCTGCTCAAAAGACACACTCCTAAAA1370.12032109044281675No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1330.11680806590434034No Hit
NNGGANNNNGNTATGNNNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1290.11329504136586395No Hit
NNGGANNNNGNTATGANNACCGTTAAGCCACACCAAATGGTGAGCCCTGC1270.11153852909662573No Hit
NAGGANNNNGNTATGNNNGCATCACTGAGGGAGGATCCTGTGGGGGAGCC1220.10714724842353024No Hit
NNGGANNNNGNTATGNNNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1210.10626899228891114No Hit
NAGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1200.10539073615429204No Hit
NNGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1190.10451248001967293No Hit
NAGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT1190.10451248001967293No Hit
NAGGANNNNGNTATGNNNAAAACCAAGTCACCTGTTGATCTGTGCAGGAA1190.10451248001967293No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1180.10363422388505383No Hit
NNGGANNNNGNTATGANNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT1180.10363422388505383No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1180.10363422388505383No Hit
NNGGANNNNGNTATGANNACAGCTTTCTGAGGGTTGTTCCAGGTGCGGTG1170.10275596775043473No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1170.10275596775043473No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1160.10187771161581564No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1160.10187771161581564No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGATTCTCAGATTCC1150.10099945548119654No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1150.10099945548119654No Hit
NNGGANNNNGNTATGANNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1150.10099945548119654No Hit
NNGGANNNNGNTATGANNACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT1140.10012119934657744No Hit
NAGGANNNNGNTATGANNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1140.10012119934657744No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)